Thin films of perfectly polar crystallites with uniaxial orientational ordering

The family of metal-organic complexes,(4-dialkylaminopyridyl)bis(acetylacetonato)zinc(II) which show perfectly polar assembly in the crystalline state are found to grow as orientationally ordered crystallites in vapor deposited thin films on glass substrates. A unique crystallographic axis of the crystallites, the longest unit cell axis in most cases, is oriented perpendicular to the substrate plane. The fact that the current observations cover a family of compounds is novel and of general significance for oriented thin film growth on amorphous substrates. Crystal structure, thin film morphology and second harmonic generation of a representative system are presented; the crystallites in the thin films do not show azimuthal orientational ordering

State-Compute Replication: Parallelizing High-Speed Stateful Packet Processing

With the slowdown of Moore's law, CPU-oriented packet processing in software will be significantly outpaced by emerging line speeds of network interface cards (NICs). Single-core packet-processing throughput has saturated. We consider the problem of high-speed packet processing with multiple CPU cores. The key challenge is state--memory that multiple packets must read and update. The prevailing method to scale throughput with multiple cores involves state sharding, processing all packets that update the same state, i.e., flow, at the same core. However, given the heavy-tailed nature of realistic flow size distributions, this method will be untenable in the near future, since total throughput is severely limited by single core performance. This paper introduces state-compute replication, a principle to scale the throughput of a single stateful flow across multiple cores using replication. Our design leverages a packet history sequencer running on a NIC or top-of-the-rack switch to enable multiple cores to update state without explicit synchronization. Our experiments with realistic data center and wide-area Internet traces shows that state-compute replication can scale total packet-processing throughput linearly with cores, deterministically and independent of flow size distributions, across a range of realistic packet-processing programs

Functional characterization of the phospholipase C activity of Rv3487c and its localization on the cell wall of Mycobacterium tuberculosis

Mycobacterium tuberculosis survives and persists for prolonged periods within its host in an asymptomatic, latent state and can reactivate years later if the host's immune system weakens. The dormant bacilli synthesize and accumulate triacylglycerol, reputed to be an energy source during latency. Among the phospholipases, phospholipase C plays an important role in the pathogenesis. Mutations in a known phospholipase C, plcC, of M. tuberculosis attenuate its growth during the late phase of infection in mice. Hydrolysis of phospholipids by phospholipase C generates diacylglycerol, a well-known signalling molecule that participates in the activation of extracellular signal-regulated kinases (ERK) through protein kinase C leading to macrophage activation. In the present study, we show that M. tuberculosis possesses an additional cell wall-associated protein, Rv3487c, with phospholipase C activity. The recombinant Rv3487c hydrolyses the substrate phosphatidylcholine and generates diacylglycerol by removing the phosphocholine. Furthermore, Rv3487c is expressed during infection as it exhibits significant humoral immunoreactivity with sera from children with tuberculosis, but not with that from adult patients

Hardware-Software Co-Design for Network Performance Measurement

Diagnosing performance problems in networks is important, for example to determine where packets experience high latency or loss. However, existing performance diagnoses are constrained by limited switch mechanisms for measurement. Alternatively, operators use endpoint information indirectly to infer root causes for problematic latency or drops. Instead of designing piecemeal solutions to work around such switch restrictions, we believe that the right approach is to co-design language abstractions and switch hardware primitives for network performance measurement. This approach provides confidence that the switch primitives are sufficiently general, i.e., they can support a variety of existing and unanticipated use cases. We present a declarative query language that allows operators to ask a diverse set of network performance questions. We show that these queries can be implemented efficiently in switch hardware using a novel programmable key-value store primitive. Our preliminary evaluations show that our hardware design is feasible at modest chip area overhead relative to existing switching chips

Evaluation of Eclipse 3D plans using an independent treatment planning system

Purpose: The goal of the current investigation was to compare complex 3D conformal plans generated on Eclipse™ treatment planning system (TPS) with independent dose calculations from radiation oncology planning system (ROPS™) TPS used as a secondary quality assurance check.Methods: Fifteen cancer patients that were treated with complex conformal treatment plans with cobalt and linac beams, using Eclipse TPS, were selected for this study. The structure sets, treatment beam data and prescription information were exported from the Eclipse TPS using DICOM-RT export. Using custom software, these data were imported into ROPS TPS. Independent dose calculation on the ROPS planning system using Clarkson summation algorithm was done. The dose volume histograms (DVH) from both planning systems were extracted and analyzed using custom software. Dose assessment was accomplished by defining criteria based on gross tumor volume (GTV) dose coverage, dose homogeneity and mean dose. For organs at risk (OAR) other than GTV, the main dose parameters were, mean dose and percentage of volume receiving 95% of prescription dose.Results: For the GTV, all 15 cases met the criteria set for the mean dose and dose homogeneity index. However, breast cases were found to have deviation in the percentage volume receiving the 95% of prescription dose.Conclusion: Using the criteria set for plan acceptance, all the 15 clinical cases were evaluated. Except for breast tangent plans, all plans passed all the criteria set. The large deviation for breast tangent plans was attributed to differences in dose calculation algorithms

Clinical presentation and predictors of outcome in patients with severe acute exacerbation of chronic obstructive pulmonary disease requiring admission to intensive care unit

BACKGROUND: Severe acute exacerbation of chronic obstructive pulmonary disease (AE-COPD) is a common reason for emergency room (ER) visit about which little has been documented from India. METHODS: Prospective study of the clinical presentation and predictors of outcome in 116 patients presenting with severe AE-COPD requiring admission to the medical intensive care unit between January 2000 and December 2004. RESULTS: Their mean age was 62.1 ± 9.8 years. There were 102 males. Mean duration of COPD was 7.2 ± 5.8 years. All males were smokers (22.3 ± 11.2 pack years); 35.2% smoked cigarettes and 64.8% smoked bidis. All women were exposed to domestic fuel. Associated co-morbid illnesses were present in 81 patients (69.8%); 53(45.7%) had one co-morbid illness and the remaining 28 (54.3%) had two or more co-morbid illnesses. Evidence of past pulmonary tuberculosis (PTB) was present in 28.4% patients; 5 patients who also had type II diabetes mellitus had active PTB. Arterial blood gas analysis revealed respiratory failure in 40 (33.8%) patients (type I 17.5% and type II 82.5%). Invasive mechanical ventilation was required in 18 patients. Sixteen (13.7%) patients died. Stepwise multivariate logistic regression analysis revealed need for invasive ventilation (OR 45.809, 95%CI 607.46 to 3.009;p < 0.001); presence of co-morbid illness (OR 0.126, 95%CI 0.428 to 0.037;p < 0.01) and hypercapnia (OR 0.114, 95%CI 1.324 to 0.010;p < 0.05) were predictors of death. CONCLUSION: Co-morbid conditions and metabolic abnormalities render the diagnosis of AE-COPD difficult and also contribute to mortality. High prevalence of past PTB and active PTB in patients with AE-COPD suggests an intriguing relationship between smoking, PTB and COPD which merits further study

Custom Integrated Circuits

Contains table of contents for Part III, table of contents for Section 1 and reports on eleven research projects.IBM CorporationMIT School of EngineeringNational Science Foundation Grant MIP 94-23221Defense Advanced Research Projects Agency/U.S. Army Intelligence Center Contract DABT63-94-C-0053Mitsubishi CorporationNational Science Foundation Young Investigator Award Fellowship MIP 92-58376Joint Industry Program on Offshore Structure AnalysisAnalog DevicesDefense Advanced Research Projects AgencyCadence Design SystemsMAFET ConsortiumConsortium for Superconducting ElectronicsNational Defense Science and Engineering Graduate FellowshipDigital Equipment CorporationMIT Lincoln LaboratorySemiconductor Research CorporationMultiuniversity Research IntiativeNational Science Foundatio

Interaction of Pyrrolobenzodiazepine (PBD) Ligands with Parallel Intermolecular G-Quadruplex Complex Using Spectroscopy and ESI-MS

Studies on ligand interaction with quadruplex DNA, and their role in stabilizing the complex at concentration prevailing under physiological condition, has attained high interest. Electrospray ionization mass spectrometry (ESI-MS) and spectroscopic studies in solution were used to evaluate the interaction of PBD and TMPyP4 ligands, stoichiometry and selectivity to G-quadruplex DNA. Two synthetic ligands from PBD family, namely pyrene-linked pyrrolo[2,1-c][1,4]benzodiazepine hybrid (PBD1), mixed imine-amide pyrrolobenzodiazepine dimer (PBD2) and 5,10,15,20-tetrakis(N-methyl-4-pyridyl)porphyrin (TMPyP4) were studied. G-rich single-stranded oligonucleotide d(5′GGGGTTGGGG3′) designated as d(T2G8), from the telomeric region of Tetrahymena Glaucoma, was considered for the interaction with ligands. ESI-MS and spectroscopic methods viz., circular dichroism (CD), UV-Visible, and fluorescence were employed to investigate the G-quadruplex structures formed by d(T2G8) sequence and its interaction with PBD and TMPyP4 ligands. From ESI-MS spectra, it is evident that the majority of quadruplexes exist as d(T2G8)2 and d(T2G8)4 forms possessing two to ten cations in the centre, thereby stabilizing the complex. CD band of PBD1 and PBD2 showed hypo and hyperchromicity, on interaction with quadruplex DNA, indicating unfolding and stabilization of quadruplex DNA complex, respectively. UV-Visible and fluorescence experiments suggest that PBD1 bind externally where as PBD2 intercalate moderately and bind externally to G-quadruplex DNA. Further, melting experiments using SYBR Green indicate that PBD1 unfolds and PBD2 stabilizes the G-quadruplex complex. ITC experiments using d(T2G8) quadruplex with PBD ligands reveal that PBD1 and PBD2 prefer external/loop binding and external/intercalative binding to quadruplex DNA, respectively. From experimental results it is clear that the interaction of PBD2 and TMPyP4 impart higher stability to the quadruplex complex