Determination of phenolic compounds in saussurea salicifolia (l.) DC by HPLC

Byly analyzovány aglykony flavonoidů obsažené v extraktu Saussurea salicifolia (L.) DC, získaného pomocí octanu etylnatého. Analýza byla provedena metodou HPLC přístrojem "Agilent" 1100 na sloupci stacionární fáze Alltech C18 ?m, s použitím detektoru UV254, rychlost toku mobilní fáze: 1 ml/min, mobilní fáze: acetonitril : voda : kyselina octová (36:65:1). Těmito analýzami bylo nalezeno jedenáct aglykonů flavonoidů. Kvantifikace separovaných složek byla provedena dle standardů. Bylo zjištěno, že Saussurea salicifolia z významných flavonoidních aglykonů obsahuje quercetin (28.62%) a kaempferol (3.01%).The flavonoid aglycone content of ethyl acetate fraction of Saussurea salicifolia (L.) DC was analysed by the HPLC ?Agilent? 1100 with Alltech C18 ?m column, UV254 detector, flow rate: 1 ml/min, mobile phase: acetonitrile: water:acetic acid (36:65:1). Eleven flavonoid aglycones in the ethyl acetate fraction of Saussurea salicifolia were detected by HPLC analysis. The quantification of each compound was done according to the peak area measurements which were reported in calibration curves of the corresponding standards. The result revealed that Saussurea salicifolia consists of flavonoid aglycones of quercetin (28.62%) and kaempferol (3.01%)

Anticancer activity of lignan from the aerial parts of saussurea salicifolia (l.) DC.

Nadzemní části Saussurea salicifolia (L.) DC byly analyzovány na obsah lignanů a flavonoidů po rozpuštění etanolového extraktu v chloroformu a n-butanolu. Vlastní identifikace fenolických sloučenin v chloroformové a n-butanolové frakce byly provedeny na zařízení Dionex HPLC-DAD s gradientovou elucí soustavou vodoa-metanol při 4 různý vlnových délkách (235 nm, 254 nm, 280 nm a 340 nm), s online použitím UV a LC-MS detekce. V chloroformové frakci převládal lignan s antirakovinnou aktivitou 9-OH-pinoresinol, kdežto v n-butanoloné frakci byly patrny hlavně hlavně flavonoidní glykosidy s protizánětlivými účinky jako quercetin-3-o-galactosid a apigenin-7-o-rhamnosid. Dodatečně, byl v n-butanoloné frakci nalezen také 9-OH-pinoresinol. Protirakovinné testy byly prováděny v lymfatických buňkách myší s leukemií L5178Y v koncentraci testované látky 10 ?g/ml. Surový etanolový extrakt S. salicifolia redukoval růst leukemických myší lymfatických buněk L5178Y až na 23.8%.Aerial parts of Saussurea salicifolia (L.) DC were studied for their lignan and flavonoids in solvent chloroform and n-butanol of ethanolic extract. Isolation and identification of phenolic compounds of the chloroform and n-butanol fractions were performed with Dionex HPLC-DAD system with water-methanol gradients in 4 different wave lengths (235 nm, 254 nm, 280 nm and 340 nm), using online UV and LC-MS as described previously. 9-OH-pinoresinol which is a lignan with anticancer activity was dominated in the chloroform fraction, whereas mainly flavonoid glycosides like quercetin-3-o-galactoside, apigenin-7-o-rhamnoside with anti-inflammatory effect were detected in the n-butanol fraction. Additionally, 9-OH-pinoresinol was also found in the n-butanol fraction. Anticancer tests were conducted in leukemia mouse lymphoma cells L5178Y at a concentration of 10 ?g/ml of test compound. Crude ethanol extract of S. salicifolia reduced the growth of leukemia mouse lymphoma cells L5178Y to 23.8%

Biologically active natural products from Mongolian medicinal plants Scorzonera divaricata and Scorzonera pseudodivaricata

Chromatographic separation of a crude extract obtained from the aerial parts of the Mongolian medicinal plant Scorzonera divaricata yielded the two new quinic acid derivatives feruloylpodospermic acids A and B. Both compounds feature a feruloyl group and two dihydrocaffeoyl substituents. For feruloylpodospermic acid A, the dihydrocaffeic acid substituents were found esterified at positions 1 and 5 of the quinic acid moiety, while the feruloyl group was attached at position 3. For feruloylpodospermic acid B, the substituents were linked at positions 1, 3, and 4. The aerial parts of S. pseudodivaricata that are likewise used in Mongolian traditional medicine yielded two further new natural products, for which the names scorzoneric acid and scorzonerin are proposed. Scorzoneric acid is an unusual phenolic compound featuring a central tetrasubstituted phenyl ring to which a glucose unit is bound, which in turn is substituted by an esterified acyl side chain. Further substituents of the central phenyl ring system include a butan-2-one group, which is linked to a second para-substituted phenyl ring system. Scorzonerin is a matricarin-based sesquiterpene lactone that carries an esterified dihydrocoumaric acid moiety, which in turn is glycosidically bound to glucose. The structures of all new compounds were unambiguously established from NMR (1H, 13C, COSY, HMBC) spectroscopic and mass spectrometric data. The new quinic acid derivatives feruloylpodospermic acids A and B exhibited strong antioxidative activity when analyzed in the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay