Clinical Application of Transrectal Ultrasound Guided Prostate Biopsy

Objective: To analyze the clinical application of transrectal ultrasound guided prostate biopsy. Method: 100 suspected prostate cancer patients admitted to our hospital from January 2019 to January 2021 were selected and underwent transrectal ultrasound guided prostate biopsy. Based on the pathological results of the surgery, the disease detection rate (such as benign prostatic hyperplasia, prostatic intraepithelial tumor, prostate cancer, atypical adenomatous hyperplasia, etc.), visual analogue scale (VAS) scores, and Complication rate (hematuria, urinary retention, fever, vasovagal reflex, etc.), diagnostic efficacy (sensitivity, specificity, diagnostic coincidence rate, misdiagnosis rate, missed diagnosis rate, negative predictive value, positive predictive value). Result: According to the surgical and pathological results, there were 33 cases of benign prostatic hyperplasia, 11 cases of prostatic intraepithelial tumors, 7 cases of prostate cancer, and 17 cases of atypical adenomatous hyperplasia. The patients undergoing transrectal ultrasound guided prostate biopsy included 33 cases of benign prostatic hyperplasia, 11 cases of prostatic intraepithelial tumors, 7 cases of prostate cancer, and 7 cases of atypical adenomatous hyperplasia, with a disease detection rate of 98% (98/100); The patient's VAS score is (2.13 ± 0.45) points, and the incidence of complications is 3% (3/100); The sensitivity of transrectal ultrasound guided prostate biopsy was 92.50% (30/33), specificity was 90.47% (57/63), diagnostic accuracy was 94% (94/100), misdiagnosis rate was 3.17% (2/63), missed diagnosis rate was 9.09% (3/33), negative predictive value was 95% (67/70), and positive predictive value was 92.85% (26/28). Conclusion Transrectal ultrasound-guided prostate biopsy for prostate cancer has high diagnostic accuracy, low incidence of complications such as hematuria and urinary retention, light pain and high application value

Pelvic Floor Ultrasound Evaluation of the Impact of Delivery Times and Delivery Methods on the Anterior Pelvic Cavity

Objective: To evaluate the impact of delivery frequency and delivery method on the anterior pelvic cavity using pelvic floor ultrasound. Method: 200 women who gave birth in our hospital from January 2019 to January 2022 were selected as the research subjects, including 100 first-time vaginal delivery women, 50 second-time vaginal delivery women, and 50 cesarean section women each. The control group consisted of 100 women who underwent a 15 day follow-up examination after the first artificial abortion surgery in our hospital during the same period. The patients were divided into a control group, a first-time delivery group, a second-time delivery group, and a cesarean section group. Observing the changes in the anterior pelvic structure during resting state and maximum Valsalva movement through perineal ultrasound examination. Result: The distance from the bladder neck to the reference line in the resting state was significantly different between the control group, cesarean section group, first vaginal delivery group, and second vaginal delivery group (P<0.05); During the maximum Valsalva maneuver, the detection rate of bladder neck mobility, urethral rotation angle, posterior angle of bladder, and funneling of internal urethral orifice, the second vaginal delivery group, the first vaginal delivery group>the cesarean section group>the control group, and the difference between each group was statistically significant (P<0.05). During the maximum Valsalva maneuver in the first and second vaginal delivery groups, the lowest point of the bladder was located below the reference line, and there was no statistically significant difference between the two groups (P>0.05); During the maximum Valsalva maneuver in the cesarean section group and control group, the lowest point of the bladder was located on the reference line, and there was no statistically significant difference between the two groups (P>0.05); The difference between the vaginal delivery group and the cesarean section and control group was statistically significant (P<0.05). Conclusion: Different birth times and delivery methods have varying degrees of impact on the anterior pelvic cavity of women. Pelvic floor ultrasound can early evaluate these structural changes and provide reliable basis for the screening and diagnosis of pelvic floor dysfunction disease (PFD)

Predictive Effect of Pelvic Floor Ultrasound Parameters on Stress Urinary Incontinence After Cesarean Section

Objective: To explore the predictive effect of pelvic floor ultrasound parameters on stress urinary incontinence after cesarean section. Methods: The pregnant women who underwent cesarean section in our hospital from April 2021 to April 2022 were selected as the study subjects. Fifty pregnant women with SUI within 6 months after delivery were selected as the study subjects in the experimental group. However, 50 patients who underwent cesarean section for 6 months after delivery and did not initially choose SUI were selected as the control group study subjects. The experimental content was to observe the probability of PUA, BNS, LHA, BND, URA in two groups of pregnant women under resting state. To analyze the predictive effect of ultrasound parameters on SUI after cesarean section. Results: There was no significant difference in PUA and LHA between the two groups of pregnant women at rest (P>0.05); The BNS in the resting state and the maximum Valsalva state in the experimental group were significantly lower than those in the control group. Through comprehensive collection of six data, it is possible to summarize the predictive role of pelvic floor ultrasound parameters for stress urinary incontinence after cesarean section. The data obtained by the combined diagnosis method is significantly higher than the single prediction data. Conclusion: Basin ultrasound parameters have a high predictive value for postpartum SUI after cesarean section, and combining ultrasound parameters can improve the diagnostic efficacy of postpartum SUI after cesarean section

Observation of Pelvic Floor Structure in Women Undergoing Second Natural Delivery Using Intelligent Pelvic Floor Ultrasound

Objective: To observe and study the pelvic floor structure of women undergoing second natural childbirth using intelligent pelvic floor ultrasound. Methods: Fifty pregnant women who completed second natural delivery in our hospital from April 2019 to April 2020 were selected as the experimental group, while 50 pregnant women who underwent first natural delivery during the same postpartum review were selected as the control group. Analyzing the test data of the two groups of patients, use two-dimensional ultrasound to observe whether there is pelvic floor organ prolapse and related parameter abnormalities in the two groups of pregnant women, and measure the movement of the bladder neck, urethral rotation angle, and posterior bladder angle of the pregnant women under Valsalva status; Real-time three-dimensional ultrasound was used to measure the area of the levator ani muscle hiatus under resting and Valsalva conditions, and to measure the pelvic floor muscle strength of the two groups of pregnant women. Results: There was no significant difference between the two groups in bladder neck movement, urethral rotation angle, and posterior bladder angle under Valsalva status (P>0.05). There was no statistically significant difference in the area of levator ani muscle hiatus between the two groups of parturients at rest [(14.00 ± 3.76) cm2 vs (14.51 ± 3.60) cm2] and Valsalva [(24.98 ± 326) cm2 vs (25.53 ± 3.40) cm2] (P>0.05); There was no significant difference in the incidence of pelvic floor organ prolapse and related abnormalities between the two groups (P>0.05); There was no statistically significant difference between the two groups in the unqualified rate of Class I (64.07% vs 69.00%) and Class II pelvic floor muscle strength (74.58% vs 78.00%) (P>0.05). Conclusion: The incidence of abnormal pelvic floor structure in women after second natural delivery is not significantly higher than that after first natural delivery, which provides imaging evidence for clinical guidance in selecting delivery methods and postpartum pelvic floor rehabilitation training for women after second natural delivery

Investigation of diverse bacteria encoding histidine decarboxylase gene in Sichuan-style sausages by culture-dependent techniques, polymerase chain reaction-denaturing gradient gel electrophoresis, and high-throughput sequencing

The diverse bacteria encoding histidine decarboxylase gene during the fermentation of Sichuan-style sausages were investigated by culture-dependent techniques, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), and high-throughput sequencing. All microbial indicators exhibited the advantages of mixed starter culture and the stability of microecosystem was more in the inoculation group than in the control group. DGGE and selected band sequencing were used to investigate the bacterial diversity of these sausages. Weissella were the main lactic acid bacteria (LAB) in the initial fermentation stage, whereas Weissella and Lactobacillus were the dominant bacteria in the later fermentation stage. After sequence alignment analysis, Enterobacter aerogenes and Citrobacter freundi were the two main bacteria encoding histidine decarboxylase gene and could produce histamine. These findings facilitate the better understanding of bacteria producing histidine decarboxylase during sausage fermentation and provide a theoretical basis for the control of histamine-producing bacteria in the process of fermented sausage processing.Peer reviewe

Green synthesis and evaluation of an iron-based metal-organic framework MIL-88B for efficient decontamination of arsenate from water

International audienceIron-containing metal-organic frameworks (MOFs) have gradually emerged as environmentally benign alternatives for reducing the levels of environmental contamination because of their advantages, such as readily obtained raw materials with low cost, nontoxic metal source with good biocompatibility, and distinguished physicochemical features e.g., high porosity, framework flexibility, and semiconductor properties. In this study, we reported an innovative strategy for synthesizing an iron-based MOF, MIL-88B, at room temperature. The novelty of this strategy was the use of ethanol as solvent and the pretreatment of dry milling with neither the bulk use of a toxic organic solvent nor the addition of extremely dangerous hydrofluoric acid or strong alkali. The synthesized MIL-88B(Fe) was evaluated as a sorbent for removing arsenate in water and it exhibited high adsorption capacity (156.7 mg g(-1)) at a low dosage. The removal capacity of trace arsenate on MIL-88B(Fe) was 32.3 mg g(-1) at a low equilibrium concentration (6.4 mu g L-1), which satisfied the arsenic threshold for drinking water. The results of Fourier transform infrared and X-ray photoelectron spectroscopy indicated that the As(V) molecules bonded with the oxygen molecules, which were coordinated with FeO clusters in the framework. This work presented the potential use of the up-scaled MIL-88B as an excellent sorbent for purifying arsenate-contaminated water

The Mycobacterium tuberculosis CRISPR-Associated Cas1 Involves Persistence and Tolerance to Anti-Tubercular Drugs

Tuberculosis remains one of the leading causes of death worldwide. Even if new antitubercular drugs are currently being developed, the rapid emergence and spread of drug-resistant strain remain a severe challenge. The CRISPR associated proteins 1 (Cas1), a most conserved endonuclease which is responsible for spacer integration into CRISPR arrays, was found deleted in many specific drug-resistant strains. The function of Cas1 is still unknown in Mycobacterium type III-A CRISPR family. In this study, the Cas1 (Rv2817c) defect was found in 57.14% of clinical isolates. To investigate the function of Cas1 in new spacer acquisition, we challenged Bacillus Calmette–Guérin (BCG) with a mycobacteriophage D29. Newly acquired spacer sequence matches D29 genome was not found by spacer deep-sequencing. We further expressed Cas1 in recombinant Mycobacterium smegmatis. We found that Cas1 increased the sensitivity to multiple anti-tuberculosis drugs by reducing the persistence during drug treatment. We also showed that Cas1 impaired the repair of DNA damage and changed the stress response of Mycobacterium smegmatis. This study provides a further understanding of Cas1 in Mycobacterium tuberculosis complex (MTBC) drug-resistance evolution and a new sight for the tuberculosis treatment