279 research outputs found

    A Hybrid of Adaptation and Dynamic Routing based on SDN for Improving QoE in HTTP Adaptive VBR Video Streaming

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    Recently, HTTP Adaptive Streaming HAS has received significant attention from both industry and academia based on its ability to enhancing media streaming services over the Internet. Recent research solutions that have tried to improve HAS by adaptation at the client side only may not be completely effective without interacting with routing decisions in the upper layers. In this paper, we address the aforementioned issue by proposing a dynamic bandwidth allocation and management architecture for streaming video flows to improve users satisfaction. We also introduce an initial cross layer hybrid method that combines quality adaptation of variable bitrate video streaming over the HTTP protocol at the client side and SDN based dynamical routing. This scheme is enabled by the Software Defined Networking architecture that is now being considered as an emerging paradigm that disassociates the forwarding process from the routing process. SDN brings flexibility and the ability to flexibly change routing solutions, in turn resulting in dynamically improving the services provided in the application layer. Our experimental results show that the proposed solution offers significantly higher overall bitrates as well as smoother viewing experience than existing methods.Comment: 14 pages, 17 figures, IJCSNS International Journal of Computer Science and Network Security, http://paper.ijcsns.org/07_book/201907/20190708.pd

    Surface display of Salmonella epitopes in Escherichia coli and Staphylococcus carnosus

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    <p>Abstract</p> <p>Background</p> <p><it>Salmonella enterica </it>serotype Enteritidis (SE) is considered to be one of the most potent pathogenic <it>Salmonella </it>serotypes causing food-borne disease in humans. Since a live bacterial vaccine based on surface display of antigens has many advantages over traditional vaccines, we have studied the surface display of the SE antigenic proteins, H:gm and SefA in <it>Escherichia coli </it>by the β-autotransporter system, AIDA. This procedure was compared to protein translocation in <it>Staphylococcus carnosus</it>, using a staphylococci hybrid vector earlier developed for surface display of other vaccine epitopes.</p> <p>Results</p> <p>Both SefA and H:gm were translocated to the outer membrane in <it>Escherichia coli</it>. SefA was expressed to full length but H:gm was shorter than expected, probably due to a proteolytic cleavage of the N-terminal during passage either through the periplasm or over the membrane. FACS analysis confirmed that SefA was facing the extracellular environment, but this could not be conclusively established for H:gm since the N-terminal detection tag (His<sub>6</sub>) was cleaved off. Polyclonal salmonella antibodies confirmed the sustained antibody-antigen binding towards both proteins. The surface expression data from <it>Staphylococcus carnosus </it>suggested that the H:gm and SefA proteins were transported to the cell wall since the detection marker was displayed by FACS analysis.</p> <p>Conclusion</p> <p>Apart from the accumulated knowledge and the existence of a wealth of equipment and techniques, the results indicate the selection of <it>E. coli </it>for further studies for surface expression of salmonella antigens. Surface expression of the full length protein facing the cell environment was positively proven by standard analysis, and the FACS signal comparison to expression in <it>Staphylococcus carnosus </it>shows that the distribution of the surface protein on each cell was comparatively very narrow in <it>E. coli</it>, the <it>E. coli </it>outer membrane molecules can serve as an adjuvant for the surface antigenic proteins and multimeric forms of the SefA protein were detected which would probably be positive for the realisation of a strong antigenic property. The detection of specific and similar proteolytic cleavage patterns for both the proteins provides a further starting point for the investigation and development of the <it>Escherichia coli </it>AIDA autotransporter efficiency.</p

    In silico mining for alkaline enzymes from metagenomic dna data of gut microbes of the lower termite Coptotermes gestroi in Vietnam

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    The high alkaline proteases, lipases, cellulases and hemicellulases are important enzymes in research and industries. In this study, using the Alcapred software, the metagenomic DNA sequences of  the gut flora of Coptotermes gestroi were analyzed to identify the enzymes that were specifically adapted to alkaline condition. The results show that 737 of 943 ORFs (accounting for 72%) encoded proteases, 154 of 214 ORFs (holding 78%) encoded lipases and 338 of 575 ORFs (accounting for 59%) encoded cellulase and hemicelluase. All those enzymes were predicted to be alkaline enzymes. This study provide an overview picture of the alkaline enzyme groups of the gut flora of C. gestroi, and provide a good database for mining, isolation of the genes to produce recombinant enzymes

    A problem of self-isolation in Japan: The relationship between self-isolation and COVID-19 community case

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    Background: The Japanese government advised mild or asymptomatic coronavirus disease-2019 (COVID-19) cases to self-isolate at home, while more severe individuals were treated at health posts. Poor compliance with self-isolation could be a potential reason for the new outbreak. Our study aimed to find out the correlation between the rising new cases of COVID-19 and home-based patients in Japan.Methods: A secondary data analysis study was conducted with the data from COVID-19-involved databases collected from Johns Hopkins University, Japanese Ministry of Health, Labour and Welfare, and Community Mobility Reports of Google. New community cases, stringency index, number of tests, and active cases were analyzed. Using a linear regression model, an independent variable was utilized for a given date to predict the future number of community cases.Results: Research results show that outpatient cases, the stringency, and Google Mobility Trend were all significantly associated with the number of COVID-19 community cases from the sixth day to the ninth day. The model predicting community cases on the eighth day (R2=0.8906) was the most appropriate showing outpatients, residential index, grocery and pharmacy index, retail and recreation index, and workplaces index were positively related (β1=24.2, 95% CI: 20.3–26.3, P<0.0001; β2=277.7, 95% CI: 171.8–408.2, P<0.0001; β3=112.4, 95% CI: 79.8–158.3, P<0.0001; β4=73.1, 95% CI: 53- 04.4, P<0.0001; β5=57.2, 95% CI: 25.2–96.8, P=0.001, respectively). In contrast, inpatients, park index, and adjusted stringency index were negatively related to the number of community cases (β6=-2.8, 95% CI: -3.9 – -1.6, P<0.0001; β7=-33, 95% CI: -43.6 – -27, P<0.0001; β8=-14.4, 95% CI: -20.1– -12, P<0.0001, respectively).Conclusion: Outpatient cases and indexes of Community Mobility Reports were associated with COVID-19 community cases

    The effect of polyamine 70000 (BT70) on the zinc plating process in the non-cyanide alkaline plating bath

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    The effect of polyamine having molecular weight 70,000u (BT70) in the non-cyanide alkaline plating bath on the morphological zinc electrodeposited coating was investigated. The obtained results from the reflectance of electrodeposited zinc coating by a suitable range of electro-current density and SEM images showed that BT70 had effect on the zinc plating in comparison with the zinc deposits from plating bath without BT70. The electrodeposited coating surface was rough and poor adhesion. However, adding BT70 into the plating bath led to the surface roughness of electrodeposited coating and grand size being reduced. Zinc electrodeposited coating from a plating bath containing BT70 at 0.5 g/L, respectively, experienced the highest reflectance, equal 46 % of the electrodeposited coating from the bath containing commercial additives. Hull method showed that the zinc deposited coating surface became smoother with the presence of polyamine in non-cyanide alkaline zinc plating solution. The zinc deposited coating had a semi-gloss scope at 10 A/dm2. If the BT70 content increased, the semi-gloss scop and the gloss of samples’ surfaces also grew up. Keywords. Polyamine, additive, non-cyanide alkaline zinc plating, zinc coating

    Lung Volume Reduction Surgery in Patients with Heterogenous Emphysema: Selecting Perspective

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    BACKGROUND: Lung volume reduction surgery (LVRS) was introduced to alleviate clinical conditions in selected patients with heterogenous emphysema. Clarifying the most suitable patients for LVRS remained unclear. AIM: This study was undertaken to specifically analyze the preoperative factor affecting to LVRS. METHODS: The prospective study was conducted at 103 Military Hospital between July 2014 and April 2016. Severe heterogenous emphysema patients were selected to participate in the study. The information, spirometry, and body plethysmographic pulmonary function tests in 31 patients who underwent LVRS were compared with postoperative outcomes (changing in FEV1 and CAT scale). RESULTS: Of the 31 patients, there was statistically significant difference in the outcome of functional capacity, lung function between two groups (FEV1 ≤ 50% and &gt; 50%) (∆FEV1: 22.46 vs 18.32%; p = 0.042. ∆CAT: 6.85 vs 5.07; p = 0.048). Changes of the FEV1 and CAT scale were no statistically significant differences in three groups residual volume. Patients with total lung capacity &lt; 140% had more improved than others (∆FEV1: 23.81 vs 15.1%; p = 0.031). CONCLUSION: Preoperative spirometry and body plethysmographic pulmonary function tests were useful measures to selected severe heterogenous emphysema patients for LVRS. Patients with FEV1 ≤ 50%, TLC in the range of 100-140% should be selected

    Results of acute cerebral infarction treatment with hyperbaric oxygen therapy, 2020–2022

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    Background: Cerebral stroke is the third leading cause of death after cardiovascular disease, cancerand the leading cause of disability for patients. Hyperbaric oxygen is a non-drug treatment that hasthe potential to improve brain function for patients with ischaemic stroke. The objective of this study wasto evaluate the results of treatment of acute cerebral infarction with hyperbaric oxygen therapy (HBOT).Materials and methods: This was a case-control study. One hundred ninety-five patients diagnosed withcerebral infarction, with signs of onset within 24 hours, were treated at the Centre for Underwater Medicineand Hyperbaric Oxygen of Vietnam National Institute of Maritime Medicine during the period from January2020 to December 2022. Study group included 100 patients with acute cerebral infarction treated witha combination of HBOT and medication and reference group included 95 patients treated by medicationonly (antiplatelets drugs, statins, control of associated risks factors)Results: After 7 days of treatment with hyperbaric oxygen (HBO), symptoms such as headache, dizziness,nausea, sensory disturbances, and Glasgow score of the study group improved better than that of the referencegroup (p &lt; 0.01). Movement recovery in the study group was better than the reference group:the percentage of patients with mild and moderate paralysis in the study group increased higher than thatof the reference group (86.0% and 68.4%), the degree of complete paralysis of the study group decreasedmore than that of the reference group (14.0% and 31.6%). The degree of independence in daily activitiesin the study group was better than the reference group. In the study group, the percentage of patients withcomplete independence in daily life increased from 27.0% to 84.0%. In the reference group, the rate of patientswho were independent in their daily activities increased from 37.9% to 51.6%. The average numberof treatment days of the study group was 10.32 ± 2.41 days and it the reference group 14.51 ± 3.24 days.Conclusions: Hyperbaric oxygen therapy is a non-drug treatment with many good effects in the treatmentof cerebral infarction, especially acute cerebral infarction. HBOT reduces and improves functional symptoms,improves mobility, and reduces treatment time for patients
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