602 research outputs found
Sampling Distributions of Random Electromagnetic Fields in Mesoscopic or Dynamical Systems
We derive the sampling probability density function (pdf) of an ideal
localized random electromagnetic field, its amplitude and intensity in an
electromagnetic environment that is quasi-statically time-varying statistically
homogeneous or static statistically inhomogeneous. The results allow for the
estimation of field statistics and confidence intervals when a single spatial
or temporal stochastic process produces randomization of the field. Results for
both coherent and incoherent detection techniques are derived, for Cartesian,
planar and full-vectorial fields. We show that the functional form of the
sampling pdf depends on whether the random variable is dimensioned (e.g., the
sampled electric field proper) or is expressed in dimensionless standardized or
normalized form (e.g., the sampled electric field divided by its sampled
standard deviation). For dimensioned quantities, the electric field, its
amplitude and intensity exhibit different types of
Bessel sampling pdfs, which differ significantly from the asymptotic
Gauss normal and ensemble pdfs when is relatively
small. By contrast, for the corresponding standardized quantities, Student ,
Fisher-Snedecor and root- sampling pdfs are obtained that exhibit
heavier tails than comparable Bessel pdfs. Statistical uncertainties
obtained from classical small-sample theory for dimensionless quantities are
shown to be overestimated compared to dimensioned quantities. Differences in
the sampling pdfs arising from de-normalization versus de-standardization are
obtained.Comment: 12 pages, 15 figures, accepted for publication in Phys. Rev. E, minor
typos correcte
The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions
Asymmetry Function of Interstellar Scintillations of Pulsars
A new method for separating intensity variations of a source's radio emission
having various physical natures is proposed. The method is based on a joint
analysis of the structure function of the intensity variations and the
asymmetry function, which is a generalization of the asymmetry coefficient and
characterizes the asymmetry of the distribution function of the intensity
fluctuations on various scales for the inhomogeneities in the diffractive
scintillation pattern. Relationships for the asymmetry function in the cases of
a logarithmic normal distribution of the intensity fluctuations and a normal
distribution of the field fluctuations are derived. Theoretical relationships
and observational data on interstellar scintillations of pulsars (refractive,
diffractive, and weak scintillations) are compared. Pulsar scintillations match
the behavior expected for a normal distribution of the field fluctuations
(diffractive scintillation) or logarithmic normal distribution of the intensity
fluctuations (refractive and weak scintillation). Analysis of the asymmetry
function is a good test for distinguishing scintillations against the
background of variations that have different origins
FLOWERING REPRESSOR AAA(+) ATPase 1 is a novel regulator of perennial flowering in Arabis alpina
Arabis alpina is a polycarpic perennial, in which PERPETUAL FLOWERING1 (PEP1) regulates flowering and perennial traits in a vernalization-dependent manner. Mutagenesis screens of the pep1 mutant established the role of other flowering time regulators in PEP1-parallel pathways. Here we characterized three allelic enhancers of pep1 (eop002, 085 and 091) which flower early. We mapped the causal mutations and complemented mutants with the identified gene. Using quantitative reverse transcriptase PCR and reporter lines, we determined the protein spatiotemporal expression patterns and localization within the cell. We also characterized its role in Arabidopsis thaliana using CRISPR and in A. alpina by introgressing mutant alleles into a wild-type background. These mutants carried lesions in an AAA(+) ATPase of unknown function, FLOWERING REPRESSOR AAA(+) ATPase 1 (AaFRAT1). AaFRAT1 was detected in the vasculature of young leaf primordia and the rib zone of flowering shoot apical meristems. At the subcellular level, AaFRAT1 was localized at the interphase between the endoplasmic reticulum and peroxisomes. Introgression lines carrying Aafrat1 alleles required less vernalization to flower and reduced number of vegetative axillary branches. By contrast, A. thaliana CRISPR lines showed weak flowering phenotypes. AaFRAT1 contributes to flowering time regulation and the perennial growth habit of A. alpina
The Arabidopsis CERK1-associated kinase PBL27 connects chitin perception to MAPK activation
Immunolocalization of dually phosphorylated MAPKs in dividing root meristem cells of Vicia faba, Pisum sativum, Lupinus luteus and Lycopersicon esculentum
Key message In plants, phosphorylated MAPKs display
constitutive nuclear localization; however, not all
studied plant species show co-localization of activated
MAPKs to mitotic microtubules.
Abstract The mitogen-activated protein kinase (MAPK)
signaling pathway is involved not only in the cellular
response to biotic and abiotic stress but also in the regulation
of cell cycle and plant development. The role of
MAPKs in the formation of a mitotic spindle has been
widely studied and the MAPK signaling pathway was
found to be indispensable for the unperturbed course of cell
division. Here we show cellular localization of activated
MAPKs (dually phosphorylated at their TXY motifs) in
both interphase and mitotic root meristem cells of Lupinus
luteus, Pisum sativum, Vicia faba (Fabaceae) and Lycopersicon esculentum (Solanaceae). Nuclear localization
of activated MAPKs has been found in all species. Colocalization
of these kinases to mitotic microtubules was
most evident in L. esculentum, while only about 50 % of
mitotic cells in the root meristems of P. sativum and V.
faba displayed activated MAPKs localized to microtubules
during mitosis. Unexpectedly, no evident immunofluorescence
signals at spindle microtubules and phragmoplast
were noted in L. luteus. Considering immunocytochemical
analyses and studies on the impact of FR180204 (an
inhibitor of animal ERK1/2) on mitotic cells, we hypothesize
that MAPKs may not play prominent role in the
regulation of microtubule dynamics in all plant species
PsRBR1 encodes a pea retinoblastoma-related protein that is phosphorylated in axillary buds during dormancy-to-growth transition
In intact plants, cells in axillary buds are arrested at the G1 phase of the cell cycle during dormancy. In mammalian cells, the cell cycle is suppressed at the G1 phase by the activities of retinoblastoma tumor suppressor gene (RB) family proteins, depending on their phosphorylation state. Here, we report the isolation of a pea cDNA clone encoding an RB-related protein (PsRBR1, Accession No. AB012024) with a high degree of amino acid conservation in comparison with RB family proteins. PsRBR1 protein was detected as two polypeptides using an anti-PsRBR1 antibody in dormant axillary buds, whereas it was detected as three polypeptides, which were the same two polypeptides and another larger polypeptide 2 h after terminal decapitation. Both in vitro-synthesized PsPRB1 protein and lambda protein phosphatase-treated PsRBR1 protein corresponded to the smallest polypeptide detected by anti-PsRBR1 antibody, suggesting that the three polypeptides correspond to non-phosphorylated form of PsRBR1 protein, and lower- and higher-molecular mass forms of phosphorylated PsRBR1 protein. Furthermore, in vivo labeling with [32P]-inorganic phosphate indicated that PsRBR1 protein was more phosphorylated before mRNA accumulation of cell cycle regulatory genes such as PCNA. Together these findings suggest that dormancy-to-growth transition in pea axillary buds is regulated by molecular mechanisms of cell cycle control similar to those in mammals, and that the PsRBR1 protein has an important role in suppressing the cell cycle during dormancy in axillary buds
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