In vitro evaluation of antiviral activity of essential oil from <em>Zataria multiflora</em> Boiss. against Newcastle disease virus

Introduction: The study aimed to investigate the antiviral activity of Zataria multiflora (ZM) essential oil against Newcastle disease virus (NDV) on Vero cells. Methods: The cytotoxicity of ZM essential oil was evaluated by MTT assay. Cells were infected with 100 TCID50 of a field isolate of virulent NDV (JF820294.1). ZM essential oil at concentrations of 1/5000, 1/25 000, 1/125 000 or 1/625 000 was added at different times of infection: 60 minutes pre infection, simultaneously and 60 minutes post infection. Cells were evaluated morphologically. The TCID50, neutralizing index (NI) and HA titer were determined. Results: Cells treated with ZM essential oil in all concentrations 1 hour before or after infection, showed CPE similar to control virus cells. In simultaneous use, cells treated with 1/5000 concentration of the essential oil, remained morphologically normal. TCID50 values of all treatments were very close to that of control virus except for simultaneous administration at concentration of 1/5000 which was about 1000 folds lower. Virus titer in different treatments was exactly the same as control virus titer in Haemagglutination (HA) test. Conclusion: ZM essential oil has some antiviral activity on NDV in vitro, which is possibly by destruction of virus infectivity or inhibition of early phases of viral proliferation cycle.</p

A comparative study on anti-inflammatory drug combinations in domestic pigeons with experimentally induced acute arthritis

The study compares the effect of one-time administration of nonsteroidal and/or steroidal anti-inflammatory combinations by topical or intramuscular (IM) routes to pigeons with monosodium urate (MSU)-induced arthritis. Forty-five adult domestic pigeons were assigned into nine equal groups: NC, negative control; PC, positive control with arthritis; sham, sham control; T1, meloxicam + hydrocortisone; T2, dexamethasone + piroxicam; T3, meloxicam + dexamethasone; T4, hydrocortisone + piroxicam; T5, dexamethasone + hydrocortisone; T6, meloxicam + piroxicam. Arthritis was also induced in T1 to T6 birds. Meloxicam and dexamethasone were administered by IM injection and the other drugs topically right after the induction of arthritis. Different drug combinations significantly decreased one-leg standing time. Induction of arthritis significantly increased TNF-α and IL-6 levels in synovial fluid and serum corticosterone and epinephrine in the PC group. Administration of drugs to birds of Groups T1 and T5 did not significantly change corticosterone concentration, while all different drug combinations decreased epinephrine level. Drug combinations that demonstrated better analgesic effect more strongly reduced serum epinephrine concentration. Meloxicam + hydrocortisone was the most effective combination in reducing inflammatory cytokines. In conclusion, one-time combination therapy with anti-inflammatory agents was effective in the acute management of inflammatory pain due to MSU-induced arthritis in pigeons, even by the topical route

In Ovo evaluation of antiviral effects of aqueous garlic (Allium sativum) extract against a velogenic strain of Newcastle disease virus

Introduction: The antiviral property of aqueous garlic extract against a virulent velogenic field isolate of Newcastle disease virus (NDV) was investigated in a time of addition assay in embryonated chicken eggs (ECEs). Methods: The ECEs were inoculated with the lowest concentration of the virus with HA (haemagglutination) positive activity. After determination of extract toxicity in ECEs, administration of different concentrations of the extract (0.5, 1, 2, 4 mg/mL) or ribavirin (comparative control) was performed 8 hours before (pretreatment), simultaneously (cotreatment) or 8 hours after (post treatment) virus injection. Allantoic fluids were harvested for infectivity determination, transmission electron microscopy (TEM) and viral load (HA titer) assay. In vitro HA blocking activity test was also performed. Results: Allicin content of the extract was 16.6% by HPLC method. The best viability results were related to the extract in pretreatment and ribavirin in co-treatment trials (p<0.05 as compared to infected ECEs with no treatment). Pre and co-treatment assays showed better results on HA titer of garlic treated groups. In infectivity assay, the 50% embryo lethal dose (ELD50) values of NDV were roughly 500 and 50 folds of ELD50 of the untreated virus in pre and co-treatment, respectively. No change was observed in viral shapes in TEM analysis nor HA blocking activity in vitro. Conclusion: Aqueous extract of garlic shows antiviral effects against a velogenic strain of NDV in ovo accompanied by a reduction in virus infectivity and titer. These effects are most pronounced in pretreatment trial

Management of complicated crown-root fracture in central incisors using intentional replantation with 180° rotation: A case report

Introduction: Complicated crown-root fractures are rare and their treatment is complex. Numerous methods such as crown lengthening and orthodontic or surgical extrusion have been described for the treatment of crown-root fracture. The aim of this study was to report managing complicated crown-root fracture using intentional replantation with 180° rotation. Case report: This case report demonstrates successful management of complicated crown-root fracture in central incisor of a 10-year-old boy using intentional replantation with 180° rotation. Discussion: At 18-month follow-up, the replanted tooth revealed normal function with no obvious resorption

Effect of different herbal preparations on experimental viral respiratory complex of broilers: clinical, pathological and ciliary activity aspects

Introduction: Despite the extensive use of herbal preparations for treatment of viral respiratory diseases in poultry, few studies have analyzed the effectiveness of these products. This study aimed to evaluate the effects of three different herbal respiratory symptom relieving agents in broiler chickens experimentally infected with H9N2 avian influenza (AI) and infectious bronchitis (IB) viruses. Methods: A total of 175 broiler chickens were randomly assigned into 5 equal groups. Negative control (NC) group remained intact while others received H9N2-AI and IB viruses. Treatment groups (G1-G3) but not positive control (PC) birds were treated with three different herbal agents containing menthol. Clinical and pathological aspects were evaluated during the experiment. Results: Administration of these agents to challenged chickens not only did not notably decrease clinical severity, gross and histopathological lesions, but also markedly increased mortality rate in treated groups. In dead cases, cast/plug formation was a prominent feature in the trachea. Treatment with herbal agents induced an increase of more than twofold in the number of goblet cells compared to PC group. Significant ciliostasis was observed in all challenged groups regardless of treatment, while ciliary activity was not changed statistically in comparison with the mean values of PC. Conclusion: In this study administration of herbal preparations adversely affected the tracheal epithelium via enhancement of goblet cell hyperplasia. It appears that hyper-secretion of mucosa along with ciliary incompetence causes mucus stagnation followed by tracheal or bronchial obstruction and death. These findings necessitate cautious use of these products

In vitro evaluation of antiviral activity of essential oil from Zataria multiflora Boiss. against Newcastle disease virus

Introduction: The study aimed to investigate the antiviral activity of Zataria multiflora (ZM) essential oil against Newcastle disease virus (NDV) on Vero cells. Methods: The cytotoxicity of ZM essential oil was evaluated by MTT assay. Cells were infected with 100 TCID50 of a field isolate of virulent NDV (JF820294.1). ZM essential oil at concentrations of 1/5000, 1/25 000, 1/125 000 or 1/625 000 was added at different times of infection: 60 minutes pre infection, simultaneously and 60 minutes post infection. Cells were evaluated morphologically. The TCID50, neutralizing index (NI) and HA titer were determined. Results: Cells treated with ZM essential oil in all concentrations 1 hour before or after infection, showed CPE similar to control virus cells. In simultaneous use, cells treated with 1/5000 concentration of the essential oil, remained morphologically normal. TCID50 values of all treatments were very close to that of control virus except for simultaneous administration at concentration of 1/5000 which was about 1000 folds lower. Virus titer in different treatments was exactly the same as control virus titer in Haemagglutination (HA) test. Conclusion: ZM essential oil has some antiviral activity on NDV in vitro, which is possibly by destruction of virus infectivity or inhibition of early phases of viral proliferation cycle