ULTRASTRUCTURAL CHANGES IN Schistosoma mansoni MALE WORMS AFTER in vitro INCUBATION WITH THE ESSENTIAL OIL OF Mentha x villosa Huds

Introduction: The essential oil Mentha x villosa (MVEO) has a wide range of actions, including antibacterial, antifungal, antiprotozoal and schistosomicidal actions. The present study aimed to investigate the ultrastructural changes of MVEO on the tegument of adult Schistosoma mansoni. Materials and Methods: Different concentrations of MVEO were tested on S. mansoni adult worms in vitro. Ultrastructural changes on the tegument of these adult worms were evaluated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results: The MVEO caused the death of all worms at 500 μg mL-1 after 24 h. After 24h of 500 μg mL-1 MVEO treatment, bubble lesions were observed over the entire body of worms and they presented loss of tubercles in some regions of the ventral portion. In the evaluation by TEM, S. mansoni adult worms treated with MVEO, 500 μg mL-1, presented changes in the tegument and vacuoles in the syncytial matrix region. Glycogen granules close to the muscle fibers were visible. Conclusion: The ability of MVEO to cause extensive ultrastructural damage to S. mansoni adult worms correlates with its schistosomicidal effects and confirms earlier findings with S. mansoni

Risk factors associated with american cutaneous leishmaniasis in an endemic area of Brazil

Submitted by Adagilson Silva ([email protected]) on 2017-04-26T14:56:39Z No. of bitstreams: 1 0036-4665-rimtsp-58-e86.pdf: 117446 bytes, checksum: 70532d600684083cf9be317715aafecf (MD5)Approved for entry into archive by Adagilson Silva ([email protected]) on 2017-04-26T15:23:24Z (GMT) No. of bitstreams: 1 0036-4665-rimtsp-58-e86.pdf: 117446 bytes, checksum: 70532d600684083cf9be317715aafecf (MD5)Made available in DSpace on 2017-04-26T15:23:24Z (GMT). No. of bitstreams: 1 0036-4665-rimtsp-58-e86.pdf: 117446 bytes, checksum: 70532d600684083cf9be317715aafecf (MD5) Previous issue date: 2016-12-08Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Hospital da Clínicas. Health Sciences Center. Graduate Program in Tropical Medicine. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Hospital da Clínicas. Health Sciences Center. Graduate Program in Tropical Medicine. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de Pernambuco. Hospital da Clínicas. Health Sciences Center. Graduate Program in Tropical Medicine. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de Pernambuco. Hospital da Clínicas. Health Sciences Center. Graduate Program in Tropical Medicine. Recife, PE, Brazil.Brazil is among the top five countries worldwide regarding the number of cases of leishmaniasis, which are present in all of the regions of the country. The northeastern region continues to have higher numbers of cases every year and in the state of Pernambuco, 34% of the municipalities are endemic for this disease. The diversity of vectors, reservoirs and etiological agents, in association with socioeconomic and environmental conditions, gives rise to factors that can modify the behavior of American cutaneous leishmaniasis. Consequently, the aim of the present study was to determine the risk factors associated with American cutaneous leishmaniasis in the municipality of Timbaúba, Brazil. A case-control study was conducted. A validated questionnaire was used for data collection. The study included 58 cases and 174 controls, and they were serologically diagnosed at the Oswaldo Cruz Foundation (FIOCRUZ). Our results showed that some factors were associated with American cutaneous leishmaniasis: biological (gender), economic (work activity, hours spent away from home and water supply) and peridomestic (presence of animals). In our study, the associations of these variables with leishmaniasis were linked to precarious housing conditions and poverty, which are parameters that can be managed in order to prevent the disease in this region