Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes

Background: Several MYB genes belonging to R2R3 MYB transcription factors have been used in several plant species to enhance anthocyanin production, and have shown various expression or regulation patterns. This study focused on the effect of ectopic expression of an RsMYB1 isolated from radish ( Raphanus sativa ) on chrysanthemum cv. \u2018Shinma\u2019. Results: The RT-PCR results confirmed that RsMYB1 regulated the expression of three key biosynthetic genes (CmF3H, CmDFR, and CmANS) that are responsible for anthocyanin production in transgenic chrysanthemum, but were not detected in the non-transgenic line. In all transgenic plants, higher expression levels of key biosynthetic genes were observed in flowers than in leaves. However, the presence of RsMYB1 in chrysanthemum did not affect any morphological characteristics, such as plant height, leaf shape or size, and number of flowers. Furthermore, no anthocyanin accumulation was visually observed in the leaves and floral tissue of any of the transgenic lines, which was further confirmed by anthocyanin content estimation. Conclusion: To our knowledge, this is the first time the role of an MYB transcription factor in anthocyanin production has been investigated in chrysanthemum

Overexpression of RsMYB1 Enhances Anthocyanin Accumulation and Heavy Metal Stress Tolerance in Transgenic Petunia

The RsMYB1 transcription factor (TF) controls the regulation of anthocyanin in radishes (Raphanus sativus), and its overexpression in tobacco and petunias strongly enhances anthocyanin production. However, there are no data on the involvement of RsMYB1 in the mechanisms underlying abiotic stress tolerance, despite strong sequence similarity with other MYBs that confer such tolerance. In this study, we used the anthocyanin-enriched transgenic petunia lines PM6 and PM2, which overexpress RsMYB1. The tolerance of these lines to heavy metal stress was investigated by examining several physiological and biochemical factors, and the transcript levels of genes related to metal detoxification and antioxidant activity were quantified. Under normal conditions (control conditions), transgenic petunia plants (T2-PM6 and T2-PM2) expressing RsMYB1, as well as wild-type (WT) plants, were able to thrive by producing well-developed broad leaves and regular roots. In contrast, a reduction in plant growth was observed when these plants were exposed to heavy metals (CuSO4, ZnSO4, MnSO4, or K2Cr2O7). However, T2-PM6 and T2-PM2 were found to be more stress tolerant than the WT plants, as indicated by superior results in all analyzed parameters. In addition, RsMYB1 overexpression enhanced the expression of genes related to metal detoxification [glutathione S-transferase (GST) and phytochelatin synthase (PCS)] and antioxidant activity [superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX)]. These results suggest that enhanced expression levels of the above genes can improve metal detoxification activities and antioxidant activity, which are the main components of defense mechanism included in abiotic stress tolerance of petunia. Our findings demonstrate that RsMYB1 has potential as a dual-function gene that can have an impact on the improvement of anthocyanin production and heavy metal stress tolerance in horticultural crops

Plant Regeneration Through Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid

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Analysis and Simulation of Sinusoidal PWM Technique for Sine Wave Inverter

A sinusoidal pulse width modulation technique of generating sine wave with minimized harmonics is implemented in this paper. The technique is essentially focused on sinusoidalpulse-width modulation (SPWM) control of H-bridge DC-AC inverter stage connected in series between high voltage DC links (Vs) and output (VO). It also provides the producing of SPWM signals to control the H-bridge inverter driving stage such as single phase or three phases. This technique is mathematically modelled and simulated in Matlab. Finally, the results are verified that the output sine wave is voltage regulated and ripple less. The proposed technique “generation of sinusoidal PWM by comparing a sinusoid with a triangular wave” aims to implement of a voltage regulated sine wave inverter with ripple free and glitch free output sine wave that can be operated electronic devices efficiently

Traffic models and performance evaluation of Dynamic channel allocation of Servers for GSM/GPRS Network

This paper aims to provide a dynamic resource allocation scheme for solving the problem of blocking probability and dropping probability in the case of GSM. For this purpose, a detailed traffic model for GSM is given. This research presents a performance model of a cell in a wireless network where the effect of handoff arrival and the use of guard channels are included. And this research proposes two analytical models of multidimensional rearrangement model and overflow Equivalent Random Theory (ERT) method to improve system performance of overflow cellular mobile network

Sucrose Enhances Anthocyanin Accumulation in Torenia by Promoting Expression of Anthocyanin Biosynthesis Genes

We examined the effects of different sucrose concentrations (3%, 5%, and 7%) on anthocyanin accumulation and plant growth in wild type (WT) and transgenic (T2) torenia cultivar “Kauai Rose” overexpressing the anthocyanin regulatory transcription factors B-Peru + mPAP1 or RsMYB1. Sucrose increased anthocyanin production in both WT and transgenic plants, with higher anthocyanin production in transgenic plants compared to WT plants. Higher sucrose concentrations increased production of anthocyanin in transgenic and WT plants, with increased anthocyanin production associated with increased expression of anthocyanin biosynthesis genes. Higher sucrose concentrations reduced growth of WT and transgenic plants. Our results indicate that sucrose enhances anthocyanin production in torenia by regulating anthocyanin biosynthesis genes