23 research outputs found
Predicted effect of the mutations affecting hBD-3 on protein structure stability.
<p><sup>a</sup>predicted protein thermal stability change (∆∆G in Kcal/mol) of mutation from CUPSAT program.</p><p><sup>b</sup>relative solvent accessibility of the wild type residue computed from PoP MuSiC program.</p><p><sup>c</sup>pridected protein stability change (∆∆G in Kcal/mol) of mutation from PoP MuSiC program.</p><p>Predicted effect of the mutations affecting hBD-3 on protein structure stability.</p
Clinical data of patients diagnosed with colon cancer via colonoscopy.
<p>Clinical data of patients diagnosed with colon cancer via colonoscopy.</p
Differents structures of hBDs: hBD-1 (panel A), hBD2 (Panel B), and hBD3mutant and wild type (panel C and D).
<p>Differents structures of hBDs: hBD-1 (panel A), hBD2 (Panel B), and hBD3mutant and wild type (panel C and D).</p
Human beta defensin (hBD) protein expression in colon cancer tissues.
<p>Tissues were immunostained using specific hBD antibodies (Panel 2A). hBD- positive cells in the tissues were estimated as follows: 0 points, no positive color; 1 point, <20% positive staining; 2 points, 21‑50% positive staining; 3 points, 51–75% positive staining; and 4 points, >75% positive staining. This is presented in Panel B.</p
Human beta defensin (hBD) mRNA and protein expression in colon cancer tissues.
<p>Total cellular RNA freshly extracted from normal and colon cancer tissues was reverse transcribed into cDNA and then used to measure the hBD mRNA expression (Panel1A to 1D).</p
Summary of hBDs mutations and their nature/location found in colon cancer tissues.
<p>N = Normal colon tissue</p><p>T = Tumor</p><p>Summary of hBDs mutations and their nature/location found in colon cancer tissues.</p
Amino acid sequence alignment of four human beta defensins (panel A for hBD1, panel B for hBD2,Panel C for hBD3 and panel D for hBD4) with the amino acid sequences for their corresponding observed mutants.
<p>Mutations are highlighted in red for each hBDs gene.</p
Expression and Polymorphism of Toll-Like Receptor 4 and Effect on NF-κB Mediated Inflammation in Colon Cancer Patients
<div><p>Our aim was to evaluate the association between the expression and the polymorphism of TLR4/NF-κB pathways and colon cancer. TLR4 (<i>rs4986790</i>, <i>rs10759932</i>, <i>rs10759931</i> and <i>rs2770150</i>) were genotyped in blood samples from Colorectal patients and healthy controls. TLR4 and cytokines inflammatory expression were evaluated by real time PCR on 40 matching normal and colon tissues and the protein level by Immunohistochemistry. The high level of TLR4 expression in colon cancer tissues is mainly due to infections by bacteria in the human colon and leads to induction of an acute secretion of inflammatory cytokines mediated by NF-κB. Also, we report here a clear evidence for an association between TLR4 <i>rs10759931</i> polymorphism (OR = 0.086, CI: 0.04–0.18, P = <0.00001). This polymorphism affects the entire population without being specific to either gender or to any age group. In contrast, the <i>rs2770150</i> is associated with colon cancer in women aged over 50 years and is closely linked with the decreased levels of female sex hormones during the post-menopausal period (OR = 0.188, CI: 0.074–0.48, P = <0.00084). <i>rs10759932</i> and <i>rs4986790</i> appear to have any association with colon cancer. Our data suggest that TLR4 SNPs could possibly serve as biomarkers for decision making in colon cancer treatment.</p></div
Inflammatory cytokines expression in colon cancer tissues compared to normal matching tissues:
<p>Cytokine production was assessed by qRT-PCR on colonic cancer tissues and matching normal tissues (mean ± SD, n = 40 patients, data normalized to <i>GAPDH</i>, control (open bars), cancer (solid bars), *P<0.00 t-test)</p
Immunohistochemical staining for TRF1, TRF2, ATR, ATM, Chk1 and Chk2 in paraffin-embedded colorectal carcinoma and adjacent mucosa.
<p>Abundant expression of hTERT, TRF2, ATR, ATM and Chk2 were observed in CRC tissue compared to adjacent mucosa validating the q-PCR results. No difference was detected in TRF1 protein level between the cancerous tissue and adjacent mucosa.</p