On mycotrophy of plants at the Urals coal exploitation dumps

Приведены результаты по исследованию микотрофности в формирующихся растительных сообществах на разновозрастных участках отвалов открытых буроугольных разработок, расположенных в лесной, лесостепной и степной зонах. Во флоре исследованных отвалов преобладают микотрофные виды (66,2%). Наибольшая интенсивность микоризной инфекции обнаружена в растительных сообществах лесостепи. К югу и северу от лесостепной зоны этот показатель уменьшается. Сделана попытка объяснения этого явления экологическими условиями, сложившимися на изученных отвалах. Возраст формирующихся растительных сообществ не оказал существенного влияния на степень микотрофности отдельных видов, однако относительное количество микотрофных видов по мере усложнения фитоценозов растет, но все же не достигает величины, характерной для естественных зональных фитоценозов. Прослежена микотрофность формирующихся растительных сообществ на террикониках угольных шахт в зависимости от положения на склоне и экспозиции.The article contains results of mycotrophy investigations of plants associations forming in the differently aged areas of open brown coal elaborations dumps in the forest, forest-steppe and steppe Zones. The mycotrophy species are dominating (66,2%) in the dumps flora. The most mycorrhizal infection was found in the forest-steppe associations, it’s intensity diminishing both to the South and to the north. Ecological conditions of the dumps are proposed to be a reason for the phenomenon. The mycotrophy degree of a species is not significantly influenced by the plants .associations age. The relative number of species with mycotrophy increases while mycotrophy increases while phytocenosis become more complicated, though not reaching the figures characteristic for the zonal natural phytocenosis. Mycotrophy of plants associations forming is also influenced by the wast heap exposition and slope

Novel molecular, cytotoxical, and immunological study on promising and selective anticancer activity of Mung bean sprouts

<p>Abstract</p> <p>Background</p> <p>The anticancer and immunomodulatory activity of mung bean sprouts (MBS) and the underlying mechanisms against human cervical and hepatocarcinoma cancer cells were explored.</p> <p>Methods</p> <p>MBS cytotoxicity and MBS-induced anticancer cytokines, TNF-α and IFN-β from cancer cells, and immunological cytokines, IL-4, IFN-γ, and IL-10 from peripheral mononuclear cells (PMNC) were assessed by MTS and ELISA assays. Apoptotic cells were investigated by flow cytometry. The expression level of apoptotic genes (Bax, BCL-2, Capsases 7–9) and cell cycle regulatory genes (cyclin D, E, and A) and tumor suppressor proteins (p27, p21, and p53) was assessed by real-time qPCR in the cancer cells treated with extract IC50.</p> <p>Results</p> <p>The cytotoxicity on normal human cells was significantly different from HeLa and HepG2 cells, 163.97 ± 5.73, 13.3 ± 0.89, and 14.04 ± 1.5 mg/ml, respectively. The selectivity index (SI) was 12.44 ± 0.83 for HeLa and 11.94 ± 1.2 for HepG2 cells. Increased levels of TNF-α and IFN-β were observed in the treated HeLa and HepG2 culture supernatants when compared with untreated cells. MBS extract was shown to be an immunopolarizing agent by inducing IFNγ and inhibiting IL-4 production by PBMC; this leads to triggering of CMI and cellular cytotoxicity. The extract induced apoptosis, in a dose and time dependent manner, in treated HeLa and HepG2, but not in untreated, cells (P < 0.05). The treatment significantly induced cell cycle arrest in G0/G1 in HeLa cells. The percentage of cells in G0/G1 phase of the treated HeLa cells increased from 62.87 ± 2.1%, in untreated cells, to 80.48 ± 2.97%. Interestingly, MBS IC50 induced the expression of apoptosis and tumor suppressor related genes in both HeLa and HepG2 cells. MBS extract succeeded in inducing cdk-inhibitors, p21, p53, and p27 in HeLa cells while it induced only p53 in HepG2 cells (P < 0.05). This is a clue for the cell type- specific interaction of the studied extract. These proteins inhibit the cyclin-cdk complexes apart from the presence of some other components that might stimulate some cyclins such as cyclin E, A, and D.</p> <p>Conclusion</p> <p>MBS extract was shown to be a potent anticancer agent granting new prospects of anticancer therapy using natural products.</p