12 research outputs found
Engineering Liver-detargeted AAV9 Vectors for Cardiac and Musculoskeletal Gene Transfer
We report the generation of a new class of adeno-associated virus serotype 9 (AAV9)-derived vectors displaying selective loss of liver tropism and demonstrating potential for cardiac and musculoskeletal gene transfer applications. Random mutagenesis of residues within a surface-exposed region of the major AAV9 capsid protein yielded a capsid library with mutations clustered at the icosahedral threefold symmetry axis. Using a combination of sequence analysis, structural models, and in vivo screening, we identified several functionally diverse AAV9 variants. The latter were classified into three functional subgroups, with respect to parental AAV9 displaying: (i) decreased transduction efficiency across multiple tissues; (ii) a selective decrease in liver transduction, or (iii) a similar transduction profile. Notably, variants 9.45 and 9.61 (subgroup II) displayed 10- to 25-fold lower gene transfer efficiency in liver, while transducing cardiac and skeletal muscle as efficiently as AAV9. These results were further corroborated by quantitation of vector genome copies and histological analysis of reporter (tdTomato) gene expression. The study highlights the feasibility of generating AAV vectors with selectively ablated tissue tropism, which when combined with other targeting strategies could allow sharply segregated gene expression. Liver-detargeted AAV9 variants described herein are excellent candidates for preclinical evaluation in animal models of cardiac and musculoskeletal disease
Engraftment of a Galactose Receptor Footprint onto Adeno-associated Viral Capsids Improves Transduction Efficiency
New viral strains can be evolved to recognize different host glycans through mutagenesis and experimental adaptation. However, such mutants generally harbor amino acid changes that affect viral binding to a single class of carbohydrate receptors. We describe the rational design and synthesis of novel, chimeric adeno-associated virus (AAV) strains that exploit an orthogonal glycan receptor for transduction. A dual glycan-binding AAV strain was first engineered as proof of concept by grafting a galactose (Gal)-binding footprint from AAV serotype 9 onto the heparan sulfate-binding AAV serotype 2. The resulting chimera, AAV2G9, continues to bind heparin affinity columns but interchangeably exploits Gal and heparan sulfate receptors for infection, as evidenced by competitive inhibition assays with lectins, glycans, and parental AAV strains. Although remaining hepatotropic like AAV2, the AAV2G9 chimera mediates rapid onset and higher transgene expression in mice. Similarly, engraftment of the Gal footprint onto the laboratory-derived strain AAV2i8 yielded an enhanced AAV2i8G9 chimera. This new strain remains liver-detargeted like AAV2i8 while selectively transducing muscle tissues at high efficiency, comparable with AAV9. The AAV2i8G9 chimera is a promising vector candidate for targeted gene therapy of cardiac and musculoskeletal diseases. In addition to demonstrating the modularity of glycan receptor footprints on viral capsids, our approach provides design strategies to expand the AAV vector toolkit
Intra- and Inter-Subunit Disulfide Bond Formation Is Nonessential in Adeno-Associated Viral Capsids
The capsid proteins of adeno-associated viruses (AAV) have five conserved cysteine residues. Structural analysis of AAV serotype 2 reveals that Cys289 and Cys361 are located adjacent to each other within each monomer, while Cys230 and Cys394 are located on opposite edges of each subunit and juxtaposed at the pentamer interface. The Cys482 residue is located at the base of a surface loop within the trimer region. Although plausible based on molecular dynamics simulations, intra- or inter-subunit disulfides have not been observed in structural studies. In the current study, we generated a panel of Cys-to-Ser mutants to interrogate the potential for disulfide bond formation in AAV capsids. The C289S, C361S and C482S mutants were similar to wild type AAV with regard to titer and transduction efficiency. However, AAV capsid protein subunits with C230S or C394S mutations were prone to proteasomal degradation within the host cells. Proteasomal inhibition partially blocked degradation of mutant capsid proteins, but failed to rescue infectious virions. While these results suggest that the Cys230/394 pair is critical, a C394V mutant was found viable, but not the corresponding C230V mutant. Although the exact nature of the structural contribution(s) of Cys230 and Cys394 residues to AAV capsid formation remains to be determined, these results support the notion that disulfide bond formation within the Cys289/361 or Cys230/394 pair appears to be nonessential. These studies represent an important step towards understanding the role of inter-subunit interactions that drive AAV capsid assembly
Characterization of Yeast 18S rRNA Dimethyl Transferase, Dim1p
Eukaryotic ribosome biogenesis, a dynamic and coordinated multistep process which requires more than 150 trans-acting factors, has been intensely studied in the yeast Saccharomyces cerevisiae. This evolutionarily conserved process involves numerous cleavages of pre-rRNA, modification of nucleotides, and concomitant assembly of the ribosomal proteins onto the rRNA. Considerable information is available about the importance of conserved pre-rRNA cleavage events in ribosome biogenesis; however, very little is known about the exact role of modified nucleotides, which cluster within the functionally important regions of the ribosome. One conserved group of modifications is the dimethylation of two adjacent adenosines at the 3ยด end of the small subunit rRNA which is ubiquitously carried out by the Dim1/KsgA methyltransferase family. Although dimethylation and KsgA are dispensable for survival in bacteria, the eukaryotic enzyme Dim1 is essential because of its requirement in the early pre-rRNA processing events. Similarly, few other members of the family have also evolved to carryout a second unrelated function in the cell. Almost all of the information about Dim1 was obtained from in vivo experiments in yeast, and has been determined that it is an indispensable part of a RNA-protein complex carrying out the pre-rRNA processing. Sequence analysis clearly shows that eukaryotic and archaeal enzymes have an extra insert in their C-terminal domain which is absent in bacterial enzymes and a better understanding of Dim1\u27s function is only possible by its structural characterization which is the aim of this study. After several attempts, the yeast Dim1p was expressed under mild conditions in E. coli and purified in soluble form. Dim1p was able to methylate bacterial 30S subunits both in vivo and in vitro, indicating its ability to recognize bacterial substrate. Supporting our hypothesis, neither the bacterial nor archaeal orthologs were able to complement the processing function of Dim1p in yeast, tested using the plasmid shuffling technique. Our results suggest that the C-terminal insert of Dim1p, along with some structural features of the N-terminal domain, is important for its function in pre-rRNA processing. Further studies are required to understand the complex interactions between proteins and RNA involved in the ribosome biogenesis
Recognition of a complex substrate by the KsgA/Dim1 family of enzymes has been conserved throughout evolution
Ribosome biogenesis is a complicated process, involving numerous cleavage, base modification and assembly steps. All ribosomes share the same general architecture, with small and large subunits made up of roughly similar rRNA species and a variety of ribosomal proteins. However, the fundamental assembly process differs significantly between eukaryotes and eubacteria, not only in distribution and mechanism of modifications but also in organization of assembly steps. Despite these differences, members of the KsgA/Dim1 methyltransferase family and their resultant modification of small-subunit rRNA are found throughout evolution and therefore were present in the last common ancestor. In this paper we report that KsgA orthologs from archaeabacteria and eukaryotes are able to complement for KsgA function in bacteria, both in vivo and in vitro. This indicates that all of these enzymes can recognize a common ribosomal substrate, and that the recognition elements must be largely unchanged since the evolutionary split between the three domains of life
Characterization of Cys-to-Ser mutants.
<p>Western dot blot of different fractions of wtAAV2 and Cys-to-Ser mutants resolved on a cesium chloride gradient. Aliquots 7* and 11* represent peak fractions with genome-containing particles and empty virion shells, respectively. Note the absence of A20 antibody staining for C230S and C394S mutants. Vector genome titers determined by Q-PCR are indicated adjacent to the dot blot.</p
Proteasomal inhibition studies with wtAAV2, C230S and C394S mutant vectors.
<p>(a) Western blot analysis of capsid protein subunits in cell lysates using the B1 antibody at 18 hrs post-transfection with (+) or without (โ) proteasome inhibitor, MG132. (b) Comparison of transduction efficiency (luciferase expression) of corresponding cell lysates shows that treatment with MG132 partially restores capsid protein expression, but not infectivity (light grey bars) when compared to control (dark grey bars). Error bars represent standard deviation (nโ=โ3).</p
Further mutagenesis of Cys230 and Cys394 residues.
<p>Transduction efficiency (luciferase expression) of transfected cell lysates containing a panel of (a) C230 mutants and (b) C394 mutants was evaluated at 24 hr post-transduction in HEK293 cells. Only the C394V mutant displays transduction efficiency similar to wtAAV2 vectors, while the corresponding C230V mutant is not viable. Error bars represent standard deviation (nโ=โ3).</p
Structural analysis of cysteine residues in the AAV2 capsid VP3 subunit.
<p>(a) Locations of cysteine residues on the major capsid subunit VP3 are shown in a capsid subunit pentamer. The VP3 subunits are colored pale yellow, light blue, pale green, wheat, and light pink and the side chains of each cysteine residue are highlighted using red spheres. (b) Close up view of the positions of unpaired Cys289 and Cys361 residues within a single subunit and (d) unpaired Cys230 and Cys394 residues at the interface between two VP3 subunits at the five-fold axis of symmetry. Thiol side chains are colored red and the two VP3 subunits in pale green and light blue. Low energy snapshots from DMD simulations demonstrating the feasibility of disulfide formation between Cys289/361 pair (c) and the Cys230/394 pair (e). All images were generated using Pymolยฎ.</p