Proteasomal inhibition studies with wtAAV2, C230S and C394S mutant vectors.

Abstract

<p>(a) Western blot analysis of capsid protein subunits in cell lysates using the B1 antibody at 18 hrs post-transfection with (+) or without (−) proteasome inhibitor, MG132. (b) Comparison of transduction efficiency (luciferase expression) of corresponding cell lysates shows that treatment with MG132 partially restores capsid protein expression, but not infectivity (light grey bars) when compared to control (dark grey bars). Error bars represent standard deviation (n = 3).</p

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