Utjecaj brzine doze pulsnoga zračenja na nastanak mikronukleusa u limfocitima periferne ljudske krvi

The micronucleus assay in human peripheral blood lymphocytes is a sensitive indicator of radiation damage and could serve as a biological dosimeter in evaluating suspected overexposure to ionising radiation. Micronucleus (MN) frequency as a measure of chromosomal damage has also extensively been employed to quantify the effects of radiation dose rate on biological systems. Here we studied the effects of 8 MeV pulsed electron beam emitted by Microtron electron accelerator on MN induction at dose rates between 35 Gy min-1 and 352.5 Gy min-1. These dose rates were achieved by varying the pulse repetition rate (PRR). Fricke dosimeter was employed to measure the absorbed dose at different PRR and to ensure uniform dose distribution of the electron beam. To study the dose rate effect, blood samples were irradiated to an absorbed dose of (4.7±0.2) Gy at different rates and cytogenetic damage was quantifi ed using the micronucleus assay. The obtained MN frequency showed no dose rate dependence within the studied dose rate range. Our earlier dose effect study using 8 MeV electrons revealed that the response of MN was linear-quadratic. Therefore, in the event of an accident, dose estimation can be made using linear-quadratic dose response parameters, without adding dose rate as a correction factor.Mikronukleus-test pokazao se osjetljivim pokazateljem oštećenja u limfocitima periferne ljudske krvi te se primjenjuje kao biološki dozimetar posumnja li se na prekomjerno izlaganje ionizirajućem zračenju. Mikronukleusi kao mjera oštećenja kromosoma često se rabe za procjenu učinaka zračenja u biološkim sustavima. Ovdje je istraženo djelovanje pulsnoga elektronskoga snopa od 8 MeV, dobivenog s pomoću elektronskoga akceleratora marke Microtron, na nastanak mikronukleusa u rasponu brzina doza od 35 Gy min-1 do 352.5 Gy min-1. Brzine doza mijenjale su se mijenjajući brzinu ponavljanja pulsa (tzv. pulse repetition rate, krat. PRR). Za mjerenje apsorbirane doze pri različitim PRR-ovima rabio se Frickeov dozimetar. Dozimetrijska su mjerenja također poslužila za ujednačavanje doze elektronskoga snopa. Za istraživanje utjecaja brzine doze, uzorci krvi ozračeni tako da apsorbiraju dozu od (4.7±0.2) Gy pri različitim brzinama doze, a zatim se s pomoću mikronukleus-testa utvrdilo citogenetsko oštećenje. Pokus s pulsnim snopovima energije 8 MeV upućuje na neovisnost broja mikronukleusa o brzinama doze u rasponu ispitanome u ovom istraživanju. Naše ranije istraživanje utjecaja doze pulsnoga elektronskoga zračenja energije 8 MeV upozorilo je na linearni do kvadratni odgovor izmjerenih parametara. Stoga se akcidentalna doza može procijeniti s pomoću linearnih do kvadratnih parametara odgovora na dozu, bez potrebe za korekcijom s pomoću brzine doze

Computer Applications in Metallurgical Research

This paper outlines the current efforts in computer applications in metallurgical research at the Defence Metallurgical Research Laboratory, Hyderabad. Work being done on armour penetration studies, optimization of armour profiles for fighting vehicles, computer control of multifunction 2000 tonne forge press, drawing of processing mechanism maps, process modelling of titanium sponge production and methods of curve fitting to experimental data, is described and briefly discussed

A comparative assessment of caries risk using cariogram among smokers and smokeless tobacco users in india – a cross-sectional study

Background: A dearth of literature exists concerning utilization of the unique cariogram model for caries risk assessment in tobacco users.Objective: To assess & compare caries risk among smokers & smokeless tobacco users using Cariogram model. Methods: A descriptive cross sectional study was conducted among smokers and smokeless tobacco users of Udaipur for 3 months. Caries risk assessment was done by employing a survey proforma based on the Cariogram model. Statistical analysis included descriptive statistics, Chi-square test followed by Marascuilo procedure and Stepwise multiple linear regression with 95% confidence interval and 5% significance level. Results: Majority of the smokers (56%) portrayed high caries risk (less chance to avoid new caries) followed by smokeless Tobacco users (34%). Only 40% smokeless tobacco users had relatively high chances (>60%) of avoiding future new caries. The susceptibility sector of the cariogram model contributed primarily to caries risk in the study population. Conclusion: The study findings from the different cariogram elements converged to indicate that smokers were at maximum caries risk, followed by smokeless tobacco users and therefore Cariogram model could be a useful tool to represent caries risk among smokers and smokeless tobacco users. Keywords: Smokers, cariogram, smokeless tobacco, dental caries, sugar

AFMB-Net: DeepFake Detection Network Using Heart Rate Analysis

With advances in deepfake generating technology, it is getting increasingly difficult to detect deepfakes. Deepfakes can be used for many malpractices such as blackmail, politics, social media, etc. These can lead to widespread misinformation and can be harmful to an individual or an institution’s reputation. It has become important to be able to identify deepfakes effectively, while there exist many machine learning techniques to identify them, these methods are not able to cope up with the rapidly improving GAN technology which is used to generate deepfakes. Our project aims to identify deepfakes successfully using machine learning along with Heart Rate Analysis. The heart rate identified by our model is unique to each individual and cannot be spoofed or imitated by a GAN and is thus susceptible to improving GAN technology. To solve the deepfake detection problem we employ various machine learning models along with heart rate analysis to detect deepfakes

Assessment of Knowledge, Attitude, Behaviour and Interpersonal Factors Related to the Use of Tobacco among Youth of Udaipur City, Rajasthan, India: a Cross-Sectional Study

Background: Tobacco is the most important preventable cause of disease burden and death all over the world. Apart from being the single most important determinant of cancer and cardiovascular diseases, smoking is also a threat to oral health. The Global Youth Tobacco Survey (GYTS) as a part of Global Tobacco Surveillance System (GTSS) was developed to monitor tobacco use, elicit attitudes about tobacco, and obtain information on exposure to tobacco smoke among youth. This study aimed to assess the prevalence, knowledge, attitude, behaviour and interpersonal factors related to the use of tobacco among youth of Udaipur city, Rajasthan, India. Methods: This study was conducted among 1031, 15 to 25 year old youths studying in the different colleges of Udaipur city, Rajasthan, India. The Global Youth Tobacco Survey (GYTS) core questionnaire was used. Simple descriptive statistics were used for the data. Findings: Out of the total 1031 participants (mean age: 19.55 ± 1.35), 632 (61.2%) were male (mean age: 19.66 ± 1.36) and 399 (38.7%) were female (mean age: 19.35 ± 1.35). 493 (47.8%) were current tobacco users, the majority of which were male 411 (39.8%). 122 (11.8%) had a previous history of tobacco use, while 416 (40.3%) reported that they had never used tobacco in any form. The majority of the male participants, 305 (29.5%), were consuming tobacco daily. Majority of current, 152 (30.8%), and ever tobacco users, 122 (41.8%), smoke and chew gutkha at places of entertainment followed by smoking or chewing at school/college premises. The majority of them bought gutkha themselves, 292 (47.4%). Moreover, the majority of current tobacco users, 298 (72.5%) male and 82 (100%) female, wanted to stop smoking /gutkha chewing.Conclusion: The present study indicates that there is a high prevalence of use of tobacco among youth of Udaipur city, Rajasthan, India.Keywords: Knowledge, Attitude, Smoking

A comparative assessment of caries risk using cariogram among smokers and smokeless tobacco users in india \u2013 a cross-sectional study

Background: A dearth of literature exists concerning utilization of the unique cariogram model for caries risk assessment in tobacco users. Objective: To assess & compare caries risk among smokers & smokeless tobacco users using Cariogram model. Methods: A descriptive cross sectional study was conducted among smokers and smokeless tobacco users of Udaipur for 3 months. Caries risk assessment was done by employing a survey proforma based on the Cariogram model. Statistical analysis included descriptive statistics, Chi-square test followed by Marascuilo procedure and Stepwise multiple linear regression with 95% confidence interval and 5% significance level. Results: Majority of the smokers (56%) portrayed high caries risk (less chance to avoid new caries) followed by smokeless Tobacco users (34%). Only 40% smokeless tobacco users had relatively high chances (>60%) of avoiding future new caries. The susceptibility sector of the cariogram model contributed primarily to caries risk in the study population. Conclusion: The study findings from the different cariogram elements converged to indicate that smokers were at maximum caries risk, followed by smokeless tobacco users and therefore Cariogram model could be a useful tool to represent caries risk among smokers and smokeless tobacco users

Attitudes, Practices and Perceived Barriers in Smoking Cessation among Dentists of Udaipur city, Rajasthan, India

Background: Tobacco is one of the most important causes of morbidity and mortality. Tobacco toll in India has one-fifth of all worldwide death attributed to tobacco. There are 700000 deaths per year due to smoking and 800000-900000 per year to all forms of tobacco use of exposure in India. The role of dentist in supporting their patients to quit smoking has been recognized. The present study was conducted to know the attitudes, practices and barriers in tobacco cessation among dentists of Udaipur city (Rajasthan, India). Methods: A pretested, close-ended, self-administered, coded questionnaire was distributed among all the 262 dental health practitioners and the teaching staff. Out of 262 questionnaires distributed among the dentist, 151 dentists filled out and returned the questionnaire. Findings: The majority of the dentists (98.7%) agreed that it was their responsibility to provide smoking cessation counseling. 54.3% of dentists agreed that such discussions were too time consuming. 37.1% thought they lacked knowledge regarding this subject. 35.8% feared to an extent about patient leaving their clinic if counseled much. Conclusion: In general, the dentists were in favorable attitude in tobacco cessation counseling for the patients; however, the lacks of time and knowledge and to an extent, a fear that the patients would leave their clinic, were the main identified barriers

Effect of Anti-Smoking Legislation in Public Places

Background: The aim of this study was to evaluate the effect of anti-smoking legislation in public places and public support for smoking ban. Methods: A cross-sectional survey was conducted in public places such as market, bus/railway station, workplaces and hospitals in Udaipur, India. Informed consent was obtained. The questionnaire comprised of details about their agreement with the current anti-smoking legislation and their views on the support for smoking ban in public places. Findings: The study was conducted among 314 individuals of whom 255 (81.2%) supported the general ban on smoking in public places. Non-smokers (54.4%) agreed more than smokers that the introduction of legislation would create healthier environment (P < 0.001), second hand smoke is serious threat to health (P < 0.001), more implementation needed for current antismoking legislation (P < 0.001). Smokers (45.5%) agreed more than non-smokers that everyone has the right to smoke in public places (P < 0.001). Conclusion: Local support for a ban on smoking in public places in Udaipur is high. But there is a lack of enforcing mechanism in support of the legislation and hence complete smoking ban along with strong enforcement may provide better results

Evaluation of Salivary Calcium and Phosphorous Concentration Before and After Chewing CPP-ACP Containing Chewing Gum

Svrha: Željelo se procijeniti kolika je koncentraciju kalcija i fosfata u slini prije žvakanja žvakaće gume bez šećera s dodatkom kazein-fosfopeptida i amorfnog kalcijeva fosfata (CPP–ACP-a) pod imenom RecaldentTM te procijeniti količinu i koliko dugo ostaju određene koncentracije kalcija i fosfata u slini nakon žvakanja gume s CPP–ACP-om. Materijali i metode: Provedeno je kliničko nerandomizirano ispitivanje u skupini od 30 nasumce odabranih studenata u dobi između 18 i 25 godina. Od svih je skupljena nestimulirana slina cijeđenjem prije žvakanja gume s CCP–ACP-om i u točno određenim intervalima nakon 20-minutnog žvakanja. U uzorcima se mjerila koncentracija kalcija i fosfata. Podaci su analizirani SPSS ver.17, softverom s postavkama ponovljenog mjerenja ANOVA-om, studentskim parnim t-testom i postotnom razlikom. Rezultati: Srednja vrijednost kalcija u slini znatno se povećala neposredno nakon (Ai) žvakanja žvakaće s CCP–ACP-om, sa srednjom razlikom od 22 posto u usporedbi s osnovnom vrijednošću (B). Povećanje koncentracije kalcija u odnosu na osnovnu vrijednost mjerilo se dva sata nakon žvakanja, kada je opaženo sniženje koncentracije fosfata u slini i do jedan sat nakon žvakanja žvakaće u usporedbi s osnovnom vrijednošću (B). Zaključak: CPP kao ACP-nositelj znatno povećava koncentraciju kalcija u slini i tako olakšava remineralizaciju.Objective: To estimate the salivary concentration of calcium and phosphorous before and after chewing sugar free chewing gum containing Casein phosphopeptide-Amorphous calcium phosphate (CPP-ACP) namely RecaldentTM and to determine the extent and duration to which the salivary levels of calcium and phosphorus remain retained in saliva after chewing the CPP-ACP containing gum. Materials and Method: A non-randomized clinical trial was conducted among 30 randomly selected students aged 18 – 25 years. Unstimulated saliva was collected by draining method before and at different intervals after chewing CPP-ACP chewing gum for 20 minutes. These samples were tested for calcium and phosphorous concentration. Data were analyzed using SPSS version 17 software using Repeated measure Anova and Students paired t-test and percentage difference. Results: The mean calcium concentration of saliva increased significantly immediately after (Ai) chewing CPP-ACP containing chewing gum with the mean difference of 22% when compared to the baseline (B). A significant increase in the calcium concentration of saliva as compared to the baseline (B) was observed for up to 2 hours after chewing the gum, whereas a decrease in the phosphorus concentration in saliva for up to 1 hour after chewing the gum was observed when compared to baseline (B). Conclusion - CPP as an ACP carrier significantly increases the calcium concentration of the saliva, thereby facilitating remineralization