In-vitro Flowering in Vitex Trifolia L.

Direct organogenesis and in vitro flowering was obtained in Vitex trifolia L an endangered medicinal plant. High frequency and maximum number of multiple shoots were obtained from shoot tip explants on MS medium supplemented with BAP (3.96-15.85 µM) and IAA (5.70-22.83 µM). Regenerates, when transferred to rooting medium with IBA at concentration of 2.46-14.76 µM and IAA at concentration of 2.85-17.13 µM initiated flowering along with rooting. In vitro flowers set viable seeds. Rooted plantlets were hardened and transferred to green house with 100% survivability. This finding has significant role in Pharmaceutical industries and in vitro flowering facilities in vitro pollination and fertilization, further it also facilities in advancing the generation at much faster speed under limited progeny size in the segregating generation of Vitex trifolia L

Parallel Smith-Waterman Algorithm for Gene Sequencing

Smith-Waterman Algorithm represents a highly robust and efficient parallel computing system development for biological gene sequence. The research work here gives a deep understanding and knowledge transfer about exiting approach for gene sequencing and alignment using Smith-waterman their strength and weaknesses. Smith-Waterman algorithm calculates the local alignment of two given sequences used to identify similar RNA, DNA and protein segments. To identify the enhanced local alignments of biological gene pairs Smith-Waterman algorithm uses dynamic programming approach. It is proficient in finding the optimal local alignment considering the given scoring system. DOI: 10.17762/ijritcc2321-8169.150515

Maternal and fetal outcome of febrile morbidity in pregnancy at tertiary care level

Background: Fever in pregnancy is a common clinical problem worldwide. Effects of hyperthermia depends on the extent and duration of temperature elevation, timing of exposure in pregnancy and possibly on maternal nutritional status, comorbidities, medications, socio-economic background and several other factors. The exposure of maternal temperature has been reported to lead to cell disruptions, vascular disturbance and placental infarction which can lead to the risk of structural and functional defects in progeny. The underlying maternal cytokine polymorphism is strongly associated with both intrapartum fever and neonatal outcome. Hence there is a need to detect the various life-threatening medical complications of febrile morbidity leading to severe maternal morbidity and its impact on fetal outcome. Aim of study were to study the etiology of fever in pregnancy during the study period and to know the effect of fever on both the mother and the fetus. Methods: A prospective observational study. Results: Out of the 60 cases of fever in pregnancy, most common cause of fever was urinary tract infection (30%), followed by dengue fever (25%) and upper respiratory tract infection (23.3%). Fever was associated with complications as such PROM, PPROM, preterm labour, PPH, thrombocytopenia, anemia and abortions. Fetal outcome were preterm birth (25%), low birth weight (36%), neonatal sepsis (20%) and perinatal death (13.8%). Conclusions: Febrile morbidity in pregnancy leads to numerous maternal and fetal complications can occur due to fever in pregnancy from various causes. The most common cause of fever was UTI followed by dengue fever. The maternal and fetal complications can be avoidable if the cause for the fever is diagnosed and treated early

Use of Schiller’s test versus Pap smear to increase detection rate of cervical dysplasias

Background: Application of Lugol's iodine to cervix (Schiller's test) is named as Visual Inspection of cervix after applying Lugol's Iodine (VILI). VILI improves the detection rate of suspicious area over the cervix. The objectives of the study was to screening for early carcinoma or Dysplasia cervix by Schiller’s test & Pap smear and to compare the results of Schiller’s test with Pap smear results.Methods: The present prospective randomized control trials study was undertaken among 500 women aged between 25-65 years outpatient’s Department of gynecology in Vijayanagar Institute of Medical Sciences (VIMS) Bellary. The study was undertaken during January 2007 to June 2008. Permission for the study was obtained from the College authorities prior to commencement.Results: Out of total 500 patients screened for Pap smear and Schiller’s iodine, majority were in the age group of 31-40 years. Biopsy proven dysplasia was more common in patients with white discharge (10.8%), post-menopausal bleeding (16.6%) and in posts coital bleeding (15.3%). 180 cases were Schiller’s iodine test positive, out of which biopsy proven cases were more in non-specific chronic cervicitis (68.88%) and rest seen in cervical epithelial abnormalities ( 7.7%), Mild dysplasia (10%), Moderate and Severe dysplasia 13%. Cytological abnormalities were found in 48 (9.6%), 180 cases were Schiller’s iodine test positive (36%). Among these 180 Schiller’s positive cases 24 cases (13.33%) were CIN2 and CIN 3 lesions. 42 patients were Schiller’s test guided biopsy proven for cervical dysplasia, of which only 16 patients (8.88%) were Pap smear positive. The sensitivity of Schiller’s test and Pap smear were 97.67% and 38% respectively.Conclusions: This study reveals that Schiller’s test can be use full in low resource setting and in busy outpatient departments

Research of Physical-Chemical and Ecological Characteristics of Ukkadam Lake Water Coimbatore District, Tamil Nadu, India

Degradation of lake water quality has been seen for many years, particularly in lakes close to urban areas with human activity. The goal of the current inquiry was to identify the various physical, chemical, and biological aspects of the surface water quality of several lakes in Coimbatore, India. The significance of the sampling points was considered when choosing them. Water samples were mostly taken from open wells in and around the Coimbatore district from the following sampling locations: Ukkadam Lake. The physical-chemical characteristics, such as total dissolved solids, pH, electrical conductivity, biochemical oxygen requirement, faeces coliforms, dissolved oxygen, and turbidity, Alkalinity, Sulphate, Nitrate, Phosphate, Chlorides. The findings indicated that lake water samples taken at several locations in and around Coimbatore city were above WHO criteria

Synthesis and Characterization of Rutile TiO2Nanopowders Doped with Iron Ions

Titanium dioxide nanopowders doped with different amounts of Fe ions were prepared by coprecipitation method. Obtained materials were characterized by structural (XRD), morphological (TEM and SEM), optical (UV/vis reflection and photoluminescence, and Raman), and analytical techniques (XPS and ICP-OES). XRD analysis revealed rutile crystalline phase for doped and undoped titanium dioxide obtained in the same manner. Diameter of the particles was 5–7 nm. The presence of iron ions was confirmed by XPS and ICP-OES. Doping process moved absorption threshold of TiO2into visible spectrum range. Photocatalytic activity was also checked. Doped nanopowders showed normal and up-converted photoluminescence

Kamba Ramayanam, Which Describes Relationships and Friendships

Man has a tendency to live in groups. A closer look at the history of mankind shows that at all times, human beings have coexisted together as a group or as a great society. This quality, which is inherent in the very nature of man, forms the basis for the formation of relationships and the development and expansion of those relationships. A relationship can simply be defined as a relationship with a fellow human being. When viewed closely, the relationship consists of a vast body, namely, the blood relation and the social relation. The relationship that each of the members of the community has with others is responsible for the social movement, the formation of social institutions, and the extension of families. At this stage, this relationship and its superiority have been consistently emphasized in the literature as valuable. While ancient Tamil literature helps us to understand family relations and social relations, this article examines in detail the relationships and friendships of the Kamba Ramayana, a literary work of the Chola period, which occupies a special place in the Tamil epic tradition

In vitro shoot multiplication of a seasonal and vulnerable medicinal plant-Aerva lanata L.

The present contribution focused on standardization of in vitro propagation protocols of Aerva lanata, an important medicinal plant used in diverse traditional system of medicine. MS and L2 media supplemented with auxins and cytokinins were used to test the in vitro growth response of explants. L2 media showed positive growth response and complete plant regeneration upon subsequent subculture. Using leaf segments and shoot tips as explants, callusing, shoot multiplication and rhizogenesis were obtained. L2 media fortified with 2, 4-dichlorophenoxyacetic acid (2.5 mg L-1) and benzylaminopurine (1.5 mg L-1) was standardized as the suitable medium for highest number (17 shoots per shoot tip) of shoot production and half strength L2 media with naphthaleneacetic acid (2 mg L-1) for rooting (3.2 cm in 36 days). Hardening of rooted plants was successful on 1:1:1 ratio of Soil rite:Coco peat:Vermiculite, later plants were transferred to pots containing sterile soil and maintained in Green house then planted in the field. The survival rate was between 65 and 70. The method established could be adopted for rapid large scale micro propagation and conservation of this important vulnerable medicinal herb. © 2011 Asian Network for Scientific Information