Impact of Teaching Critical Thinking Skills on Reading Comprehension in Higher Business Education

The paper aims to analyze the development of critical thinking skills in ESL classrooms as an essential factor for effective students’ learning and providing competence in the professional environment. Critical thinking ability is mental processes, techniques, and comprehension skills that can be applied to question, analyze, and make critical decisions while appropriately learning new concepts. It also involves effective communication, managing emotions, and judging resources. While estimating the effect of developing critical thinking skills on students’ reading comprehension performance, the authors have applied the following methods: performed data collection, data analysis, non-probability sampling method and comparison method. The non-probability sampling method is based on age range, education level, professional training and language proficiency. The results of the experiment indicate the relationship between critical thinking skills and reading competence. The analysis of the research results testifies the effectiveness of critical thinking skills development to improve reading comprehension while taking the Business English Certificate reading test

Centrosome positioning in interphase cells

The position of the centrosome is actively maintained at the cell center, but the mechanisms of the centering force remain largely unknown. It is known that centrosome positioning requires a radial array of cytoplasmic microtubules (MTs) that can exert pushing or pulling forces involving MT dynamics and the activity of cortical MT motors. It has also been suggested that actomyosin can play a direct or indirect role in this process. To examine the centering mechanisms, we introduced an imbalance of forces acting on the centrosome by local application of an inhibitor of MT assembly (nocodazole), and studied the resulting centrosome displacement. Using this approach in combination with microinjection of function-blocking probes, we found that a MT-dependent dynein pulling force plays a key role in the positioning of the centrosome at the cell center, and that other forces applied to the centrosomal MTs, including actomyosin contractility, can contribute to this process

Communicative texts' modelling of engineering profiles as a methodological problem

The article presents methodological materials for teaching professional foreign language (Russian), which stimulate foreign students’ communicative competence in the sphere of educational and professional communication. The structure of texts in terms of their communicative purpose is analyzed and the subject of speech in the “fabric” of the text based on the example of texts in “General Electrical Engineering” is discussed. The proposed approach introduces international students to the particularities and traditions of the presentation of scientific and technical information adopted by Russian engineering schools as one factor in the development of language culture

Теория и практика парадигмы образования в течение всей жизни: создание программы повышения квалификации

This article points out the relevance of educational conception of the paradigm of Lifelong Learning in Russian system of education. The authors pay close attention to the conception of federal program in developing education for 2016-2020, which was fundamental in creating additional vocational training program «English for special purposes in the sphere of business and innovation», realized on the base of the International Department of Management by the National Research Tomsk State University and with the support from the European Union project 544283-TEMPUS-1-2013-1-ES-TEMPUS-JPHES «Lifelong Language Learning University Centre Network for New Career Opportunities and Personal Development”. The article describes the pilot additional vocational training program as an attempt to raise the investment in people and knowledge; promote the acquisition of basic skills, develop people’s employability and adaptability and broaden opportunities in the sphere of business and innovationВ статье рассматривается актуальность концепции обучения в течение всей жизни в российском образовании. Особое внимание авторы статьи уделяют концепции ФЦП развития образования на 2016-2020 годы, которая стала основополагающей при создании программы дополнительного профессионального образования «Английский язык для профессиональных целей в сфере бизнеса и инноваций», реализуемой на базе международного факультета управления Национального исследовательского томского государственного университета при поддержки проекта Европейского союза 544283-TEMPUS-1-2013-1-ES-TEMPUS-JPHES «Создание сети университетских языковых центров для профессионального и личностного развития человека в рамках парадигмы образования в течение всей жизни. Авторы статьи детально описывают процесс создания программы, способствующей приобретению новых знаний, формированию иноязычной языковой компетенции, и создание возможности для дальнейшего развития в сфере бизнеса и инноваций, а также результаты обучения пилотных груп

Phosphorylation controls autoinhibition of cytoplasmic linker protein-170

Author Posting. © American Society for Cell Biology, 2010. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 21 (2010): 2661-2673, doi:10.1091/mbc.E09-12-1036.Cytoplasmic linker protein (CLIP)-170 is a microtubule (MT) plus-end-tracking protein that regulates MT dynamics and links MT plus ends to different intracellular structures. We have shown previously that intramolecular association between the N and C termini results in autoinhibition of CLIP-170, thus altering its binding to MTs and the dynactin subunit p150Glued (J. Cell Biol. 2004: 166, 1003–1014). In this study, we demonstrate that conformational changes in CLIP-170 are regulated by phosphorylation that enhances the affinity between the N- and C-terminal domains. By using site-directed mutagenesis and phosphoproteomic analysis, we mapped the phosphorylation sites in the third serine-rich region of CLIP-170. A phosphorylation-deficient mutant of CLIP-170 displays an "open" conformation and a higher binding affinity for growing MT ends and p150Glued as compared with nonmutated protein, whereas a phosphomimetic mutant confined to the "folded back" conformation shows decreased MT association and does not interact with p150Glued. We conclude that phosphorylation regulates CLIP-170 conformational changes resulting in its autoinhibition.This work was supported by National Institutes of Health grant GM-25062 (to G.G.B.); Netherlands Organization for Scientific Research grants (to A. A. and N. G.); a Cancer Genomics Centre grant (to J.v.H.); and Presidential Program of Russian Academy of Sciences and RFBP grant 05-04-4915 (to E.S.N.)

YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules

<p>Abstract</p> <p>Background</p> <p>YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs.</p> <p>Results</p> <p>We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly <it>in vitro</it>. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes.</p> <p>Conclusion</p> <p>These results suggest that YB-1 may regulate microtubule assembly <it>in vivo </it>and that its interaction with tubulin may contribute to the control of mRNA translation.</p

Phosphorylation controls autoinhibition of cytoplasmic linker protein-170

Author Posting. © American Society for Cell Biology, 2010. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 21 (2010): 2661-2673, doi:10.1091/mbc.E09-12-1036.Cytoplasmic linker protein (CLIP)-170 is a microtubule (MT) plus-end-tracking protein that regulates MT dynamics and links MT plus ends to different intracellular structures. We have shown previously that intramolecular association between the N and C termini results in autoinhibition of CLIP-170, thus altering its binding to MTs and the dynactin subunit p150Glued (J. Cell Biol. 2004: 166, 1003–1014). In this study, we demonstrate that conformational changes in CLIP-170 are regulated by phosphorylation that enhances the affinity between the N- and C-terminal domains. By using site-directed mutagenesis and phosphoproteomic analysis, we mapped the phosphorylation sites in the third serine-rich region of CLIP-170. A phosphorylation-deficient mutant of CLIP-170 displays an "open" conformation and a higher binding affinity for growing MT ends and p150Glued as compared with nonmutated protein, whereas a phosphomimetic mutant confined to the "folded back" conformation shows decreased MT association and does not interact with p150Glued. We conclude that phosphorylation regulates CLIP-170 conformational changes resulting in its autoinhibition.This work was supported by National Institutes of Health grant GM-25062 (to G.G.B.); Netherlands Organization for Scientific Research grants (to A. A. and N. G.); a Cancer Genomics Centre grant (to J.v.H.); and Presidential Program of Russian Academy of Sciences and RFBP grant 05-04-4915 (to E.S.N.)

Centering and Shifting of Centrosomes in Cells

Centrosomes have a nonrandom localization in the cells: either they occupy the centroid of the zone free of the actomyosin cortex or they are shifted to the edge of the cell, where their presence is justified from a functional point of view, for example, to organize additional microtubules or primary cilia. This review discusses centrosome placement options in cultured and in situ cells. It has been proven that the central arrangement of centrosomes is due mainly to the pulling microtubules forces developed by dynein located on the cell cortex and intracellular vesicles. The pushing forces from dynamic microtubules and actomyosin also contribute, although the molecular mechanisms of their action have not yet been elucidated. Centrosomal displacement is caused by external cues, depending on signaling, and is drawn through the redistribution of dynein, the asymmetrization of microtubules through the capture of their plus ends, and the redistribution of actomyosin, which, in turn, is associated with basal-apical cell polarization

Практический подход к концепции «Lifelong learning» в рамках языкового профессионального образования (на примере специальности «Государственное и муниципальное управление»)

The article discusses a topical issue of developing a system of ongoing education, which views a foreign language acquisition as an impartial element of enhancement of communicative and intercultural competences level in the context of specialization and professional career, including the sphere of state and municipal governance. The authors of the article present a detailed description of the syllabus for a continuing professional education program “English for Civil Servants in State and Municipal Governance”, which is pursued at National Research Tomsk State University on the premises of International Management Department with the support from the European Union project 544283-TEMPUS-1-2013-1-ES-TEMPUS-JPHES “Lifelong Language Learning University Centre Network for New Career Opportunities and Personal Development”. The key feature of the program offered to adult students who are already experts in their field is the use of role plays and communicative tasks directly connected with their field of expertise, allowing them to practice using their newly acquired knowledge and skills in a foreign language in situations of their daily working life thus preparing them for professional interaction in a foreign language and making them compatible in the international job market and giving them an opportunity to develop professionally through communication with their colleagues and exchange experienceСтатья посвящена актуальной теме развития системы непрерывного образования, в рамках которого знание иностранных языков, в частности английского языка, является неотъемлемым атрибутом повышения уровней коммуникативной и межкультурной компетенции в контексте специальности и профессиональной деятельности, в том числе и в области государственного и муниципального управления. Особое внимание авторы статьи уделяют описанию программы дополнительного профессионального образования «Английский язык для государственных и муниципальных служащих», реализуемой на базе международного факультета управления Национально исследовательского томского государственного университета при поддержки проекта Европейского союза 544283-TEMPUS-1-2013-1-ES-TEMPUS-JPHES «Создание сети университетских языковых центров для профессионального и личностного развития человека в рамках парадигмы «образование в течение всей жизни». Представленная программа позволяет слушателям, которые уже являются экспертами в области государственного и муниципального управления, использовать полученные знания и навыки на иностранном языке в ситуациях повседневного общени