Prevalence of blaSHV genes in clinical isolates of Klebsiella pneumoniae at Saint Camille medical Center in Ouagadougou. Isolation of blaSHV11-like gene

Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli) representative of pathogenic species and resistant to β-lactam antibiotics are investigated to isolate the genes responsible of β--lactamase activity. The use of engineering techniques enables us to show the widespread of blaSHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted an atypical blaSHV-11 gene. Key words: β--Lactamases, Klebsiella pneuminiae, blaSHV gene, Saint Camille medical centre, Ouagadougou. African Journal of Biotechnology Vol.3(9) 2004: 477-48

Identification of metallo-b-lactamase from a clinical isolate at Saint Camillle Hospital Center of Ouagadougou/ Burkina Faso

A metallo-b-lactamase was identified from a clinical sample. The host bacteria was identified to be Chryseobacterium Indologenes. This preliminary characterization of the enzyme is reported here.African Journal of Biotechnology Vol. 4 (3), pp. 286-288, 200

Short Communication - Identification of metallo-b-lactamase from a clinical isolate at Saint Camillle medical Center of Ouagadougou, Burkina Faso

A metallo-β-lactamase was identified from a clinical sample. The host bacteria was identified to be Chryseobacterium Indologenes. This preliminary characterization of the enzyme is reported here

Full Length Research Paper - Prevalence of blaSHV genes in clinical isolates of Klebsiella pneumoniae at Saint Camille medical Center in Ouagadougou. Isolation of blaSHV11-like gene

Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli ) representative of pathogenic species and resistant to β-lactam antibiotics are investigated to isolate the genes responsible of β-lactamase activity. The use of engineering techniques enables us to show the widespread of blaSHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted an atypical blaSHV-11 gene

Évaluation du Forum national de la recherche scientifique et des innovations technologiques (FRSIT) du 10 au 30 septembre 2005

Le Forum national de la Recherche Scientifique et des Innovations Technologiques (FRSIT) est une institution du Burkina Faso créée pour permettre de prendre en considération les résultats de recherche pour un développement endogène du pays. Il marque aussi l’engagement des autorités burkinabé pour une meilleure valorisation des innovations et des inventions. Le forum qui se réunit tous les 2 ans a été marqué par d’importantes évolutions tant sur le plan institutionnel qu’organisationnel, dont la fusion avec le Salon National de l’Invention et de l’Innovation (SNII). Toutes ses éditions ont été marquées par une forte participation des inventeurs, chercheurs, opérateurs privés et décideurs, des participants de pays d’Afrique de l’Ouest et du Centre, mais également de centres internationaux de recherche. Cependant, malgré les succès enregistrés, un certain nombre d’insuffisances ont été relevées et des préoccupations ont été exprimées, faisant l’objet de recommandations à l’issue des éditions..

Polyphenol content and antioxidant activity of fourteen wild edible fruits from Burkina Faso. Molecules 2008

Abstract: A total of fourteen (14) species of wild edible fruits from Burkina Faso were analyzed for their phenolic and flavonoid contents, and their antioxidant activities using the DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic and total flavonoid levels were significantly higher in the acetone than in the methanol extracts. Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content, followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana and Lannea microcarpa. Significant amounts of total phenolics were also detected in the other fruit species in the following order of decreasing levels: Tamarindus indica> Sclerocarya birrea> Dialium guineense> Gardenia erubescens> Diospyros mespiliformis> Parkia biglobosa> Ficus sycomorus> Vitellaria paradoxa. Detarium microcarpum fruit also showed the highest antioxidant activity using the three antioxidant assays. Fruits with high antioxidant activities were also found to possess high phenolic and flavonoid contents. There was a strong correlation between total phenolic and flavonoid levels and antioxidan

IND-6, a Highly Divergent IND-Type Metallo-β-Lactamase from Chryseobacterium indologenes Strain 597 Isolated in Burkina Faso ▿

The genus Chryseobacterium and other genera belonging to the family Flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. Several species of Flavobacteriaceae, including Chryseobacterium indologenes, are naturally resistant to β-lactam antibiotics (including carbapenems), due to the production of a resident metallo-β-lactamase. Although C. indologenes presently constitutes a limited clinical threat, the incidence of infections caused by this organism is increasing in some settings, where isolates that exhibit multidrug resistance phenotypes (including resistance to aminoglycosides and quinolones) have been detected. Here, we report the identification and characterization of a new IND-type variant from a C. indologenes isolate from Burkina Faso that is resistant to β-lactams and aminoglycosides. The levels of sequence identity of the new variant to other IND-type metallo-β-lactamases range between 72 and 90% (for IND-4 and IND-5, respectively). The purified enzyme exhibited N-terminal heterogeneity and a posttranslational modification consisting of the presence of a pyroglutamate residue at the N terminus. IND-6 shows a broad substrate profile, with overall higher turnover rates than IND-5 and higher activities than IND-2 and IND-5 against ceftazidime and cefepime