77 research outputs found
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The H-abc domain of syntaxin 3 is a ubiquitin binding domain
Syntaxins are a family of membrane-anchored SNARE proteins that are essential components required for membrane fusion in eukaryotic intracellular membrane trafficking pathways. Syntaxins contain an N-terminal regulatory domain, termed the H-abc domain that is not highly conserved at the primary sequence level but folds into a three-helix bundle that is structurally conserved among family members. The syntaxin H-abc domain has previously been found to be structurally very similar to the GAT domain present in GGA family members and related proteins that are otherwise completely unrelated to syntaxins. Because the GAT domain has been found to be a ubiquitin binding domain we hypothesized that the H-abc domain of syntaxins may also bind to ubiquitin. Here, we report that the H-abc domain of syntaxin 3 (Stx3) indeed binds to monomeric ubiquitin with low affinity. This domain binds efficiently to K63-linked poly-ubiquitin chains within a narrow range of chain lengths but not to K48-linked poly-ubiquitin chains. Other syntaxin family members also bind to K63-linked poly-ubiquitin chains but with different chain length specificities. Molecular modeling suggests that residues of the GGA3-GAT domain known to be important for ionic and hydrophobic interactions with ubiquitin may have equivalent, conserved residues within the H-abc domain of Stx3. We conclude that the syntaxin H-abc domain and the GAT domain are both structurally and functionally related, and likely share a common ancestry despite sequence divergence. Binding of Ubiquitin to the H-abc domain may regulate the function of syntaxins in membrane fusion or may suggest additional functions of this protein family
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Endometriosis and lower urinary tract symptoms: association or causation?
MP37-12 COVID-19 VACCINATION IS ASSOCIATED WITH A DECREASED RISK OF ORCHITIS AND/OR EPIDIDYMITIS IN MEN
Vaccine hesitancy is a major public health obstacle to fighting the ongoing COVID‐19 epidemic. Due to studies that show COVID‐19 infection can affect sperm parameters and lead to orchitis, the public are concerned about the effect of the COVID vaccines on male reproduction. In this study, we investigated the association between COVID‐19 vaccination and risk of developing orchitis and/or epididymitis outcomes in a cohort of men using a large, US‐based, electronic health record database. After balancing for confounding variables, we found that receiving at least 1 COVID‐19 vaccine is associated with a decreased risk of developing orchitis and/or epididymitis
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Comment on: Prior COVID-19 infection associated with increased risk of newly diagnosed erectile dysfunction
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MP38-19 PREVALENCE OF TESTOSTERONE DEFICIENCY AND SECONDARY POLYCYTHEMIA IN MEN WITH OBSTRUCTIVE SLEEP APNEA
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SECONDARY POLYCYTHEMIA IN MEN RECEIVING TESTOSTERONE THERAPY INCREASES RISK OF MAJOR ADVERSE CARDIOVASCULAR EVENTS AND VENOUS THROMBOEMBOLISM: INSIGHTS FROM A GLOBAL HEALTH RESEARCH NETWORK
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MP76-05 REVISITING RISK OF TESTOSTERONE THERAPY: ASSESSING THE IMPACT OF SUPRATHERAPEUTIC LEVELS ON MACE AND MORTALITY
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PENILE PROSTHESIS IS PROTECTIVE AGAINST MAJOR DEPRESSIVE DISORDER IN MEN WITH ERECTILE DYSFUNCTION: A MULTICENTER RESEARCH NETWORK STUDY
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PD30-04 ERECTILE DYSFUNCTION IS ASSOCIATED WITH MAJOR DEPRESSIVE DISORDER: INSIGHTS FROM A MULTINATIONAL REGISTRY 8.6.1
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