Insights on mauritiana-like Elements Diversity in Mayetiola destructor and M. hordei (Diptera: Cecidomyiidae)

Mariner-like elements (MLEs) are class II transposons belonging to the Tc1-mariner family, that have successfully invaded many insect genomes. In the current study, the availability of the Hessian fly Mayetiola destructor genome has enabled us to perform in silico analysis of MLEs using as query the previously described mariner element (Desmar1) belonging to mauritiana subfamily. Eighteen mauritiana-like elements were detected and were clustered into three main groups named Desmar1-like, MauCons1 and MauCons2. Subsequently, in vitro analysis was carried out to investigate mauritiana-like elements in M. destructor as well as in Mayetiola hordei using primers designed from TIRs of the previously identified MLEs. PCR amplifications were successful and a total of 12 and 17 mauritiana-like elements were discovered in M. destructor and M. hordei, respectively. Sequence analyses of mauritiana-like elements obtained in silico and in vitro have showed that MauCons1 and MauCons2 elements share low similarity with Desmar1 ranging from 50% to 55% suggesting different groups under mauritiana subfamily have invaded the genomes of M. destructor and M. hordei. These groups are likely inherited by vertical transmission that subsequently underwent different evolutionary histories. This work describes new mauritiana-like elements in M. destructor that are distinct from the previouslydiscovered Desmar1 and provides the first evidence of MLEs belonging to mauritiana subfamily in M. hordei.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Exploration of bacterial diversity in leaves and rhizosphere soil of flood affected and unaffected apricot trees

International audiencePlants harbor diverse and taxonomically structured communities of protective microorganisms that confer beneficial characteristics in plant growth and protection against biotic and abiotic stresses. The aim of this study is to characterize the composition of endophytic bacterial communities in leaves and rhizobacterial communities of the flood affected and unaffected apricot trees by 16S rRNA genes metabarcoding. Results showed a slight bacterial diversity reduction in flood affected condition compared to the unaffected one. The relative bacterial abundance analysis showed that the bacterial taxa were almost uniformly distributed between the leaves. However, a clear differential taxa abundance was observed in rhizosphere soil which may be associated with apricot tree environmental status. In fact, the flood affected rhizosphere soil showed a significant increase in several bacterial taxa including particularly Bacillusfrigoritolerans and Pseudoarthrobacterphenanthrenivorans which are known to be beneficial to soil. However, some Actinobacteria genera that promote growth of plants, including Nocardioidessp.,Streptomycessp.,BlastococcusandGeodermatophilus were decreased in abundance. This distribution of abundance and diversity of the bacterial community in the flood affected tree can be correlated to abiotic stress. These results provide new insights into rhizosphere-associated microbes that are likely playing a crucial role in plant growth and survival