Determining level of PCBs in skin and muscle tissue of Cyprinus carpio and Esox lucius in Anzali Wetland (Abkenar)

The aim of this research is to determine the levels of polycholoro biphenil (PCBs) in skin and muscle tissues of common carp (Cyprinus carpio) and common pike (Esox lucius) fishes in the west part of Anzali wetland (Abkenar) in the two seasons, autumn 2009 and winter 2010. In each seasons, nine fishes from each species were selected randomly. The samples, transferred in cool condition and the muscles and skins of them were removed. All of the preparations organs clean up and PCBS analysis were carried with GC-ECD (Gas chromatography). Mean concentrations of PCBS were 24.36пg/g dry.w in skin 14.36пg/g dry.w in muscle tissue for E. lucius and 21.84пg/g dry.w in skin and 20.86пg/g dry.w in muscle tissue of C. carpio. In the autumn, the values was less than FDI standard and food standard Australia/AZ MRL and skin tissue of E. lucius and muscle tissue of C. carpio was over of US EPA standard values. Mean concentration of PCBS were 29.43пg/g dry.w in skin and 10.10ng/g dry.w in muscle tissue for E. lucius and 33.06пg/g dry.w in skin and 11.00пg/g dry.w in muscle tissue for C. carpio. In the winter, the value was less than FDI standard and food standard Australia /AZ MRL and skin tissue of E. lucius and skin tissue of C. carpio was over of US EPA standard values. Mean concentration of PCBs in water in the autumn (0.0042ppb) was less than recommended water quality Criteria E.P.A and in the winter (0.1938ppb) was over recommended water quality Criteria E.P.A. Mean concentration of PCBs in sediment in the autumn (4.89ng/g dry.w) and winter (4.48ng/g dry.w) was less than Interim Canadian Sediment Quality Guidelines. There were significant differences between E. lucius mussels and C. carpio skin tissue in two seasons (autumn and winter) in water and ingredient suspension. But in sediments, no significant difference was detected in autumn and winter

Energy Consumption, Carbon Emissions and Global Warming Potential of Wolfberry Production in Jingtai Oasis, Gansu Province, China

During the last decade, China's agro-food production has increased rapidly and been accompanied by the challenge of increasing greenhouse gas (GHG) emissions and other environmental pollutants from fertilizers, pesticides, and intensive energy use. Understanding the energy use and environmental impacts of crop production will help identify environmentally damaging hotspots of agro-production, allowing environmental impacts to be assessed and crop management strategies optimized. Conventional farming has been widely employed in wolfberry (Lycium barbarum) cultivation in China, which is an important cash tree crop not only for the rural economy but also from an ecological standpoint. Energy use and global warming potential (GWP) were investigated in a wolfberry production system in the Yellow River irrigated Jingtai region of Gansu. In total, 52 household farms were randomly selected to conduct the investigation using questionnaires. Total energy input and output were 321,800.73 and 166,888.80 MJ ha−1, respectively, in the production system. The highest share of energy inputs was found to be electricity consumption for lifting irrigation water, accounting for 68.52%, followed by chemical fertilizer application (11.37%). Energy use efficiency was 0.52 when considering both fruit and pruned wood. Nonrenewable energy use (88.52%) was far larger than the renewable energy input. The share of GWP of different inputs were 64.52% electricity, 27.72% nitrogen (N) fertilizer, 5.07% phosphate, 2.32% diesel, and 0.37% potassium, respectively. The highest share was related to electricity consumption for irrigation, followed by N fertilizer use. Total GWP in the wolfberry planting system was 26,018.64 kg CO2 eq ha−1 and the share of CO2, N2O, and CH4 were 99.47%, 0.48%, and negligible respectively with CO2 being dominant. Pathways for reducing energy use and GHG emission mitigation include: conversion to low carbon farming to establish a sustainable and cleaner production system with options of raising water use efficiency by adopting a seasonal gradient water pricing system and advanced irrigation techniques; reducing synthetic fertilizer use; and policy support: smallholder farmland transfer (concentration) for scale production, credit (small- and low-interest credit) and tax breaks

Consensus Middle East and North Africa Registry on Inborn Errors of Immunity

Background: Inborn errors of immunity (IEIs) are a heterogeneous group of genetic defects of immunity, which cause high rates of morbidity and mortality mainly among children due to infectious and non-infectious complications. The IEI burden has been critically underestimated in countries from middle- and low-income regions and the majority of patients with IEI in these regions lack a molecular diagnosis. Methods: We analyzed the clinical, immunologic, and genetic data of IEI patients from 22 countries in the Middle East and North Africa (MENA) region. The data was collected from national registries and diverse databases such as the Asian Pacific Society for Immunodeficiencies (APSID) registry, African Society for Immunodeficiencies (ASID) registry, Jeffrey Modell Foundation (JMF) registry, J Project centers, and International Consortium on Immune Deficiency (ICID) centers. Results: We identified 17,120 patients with IEI, among which females represented 39.4%. Parental consanguinity was present in 60.5% of cases and 27.3% of the patients were from families with a confirmed previous family history of IEI. The median age of patients at the onset of disease was 36 months and the median delay in diagnosis was 41 months. The rate of registered IEI patients ranges between 0.02 and 7.58 per 100,000 population, and the lowest rates were in countries with the highest rates of disability-adjusted life years (DALY) and death rates for children. Predominantly antibody deficiencies were the most frequent IEI entities diagnosed in 41.2% of the cohort. Among 5871 patients genetically evaluated, the diagnostic yield was 83% with the majority (65.2%) having autosomal recessive defects. The mortality rate was the highest in patients with non-syndromic combined immunodeficiency (51.7%, median age: 3.5 years) and particularly in patients with mutations in specific genes associated with this phenotype (RFXANK, RAG1, and IL2RG). Conclusions: This comprehensive registry highlights the importance of a detailed investigation of IEI patients in the MENA region. The high yield of genetic diagnosis of IEI in this region has important implications for prevention, prognosis, treatment, and resource allocation

Self-organizing maps of typhoon tracks allow for flood forecasts up to two days in advance

[[abstract]]Typhoons are among the greatest natural hazards along East Asian coasts. Typhoon-related precipitation can produce flooding that is often only predictable a few hours in advance. Here, we present a machine-learning method comparing projected typhoon tracks with past trajectories, then using the information to predict flood hydrographs for a watershed on Taiwan. The hydrographs provide early warning of possible flooding prior to typhoon landfall, and then real-time updates of expected flooding along the typhoon’s path. The method associates different types of typhoon tracks with landscape topography and runoff data to estimate the water inflow into a reservoir, allowing prediction of flood hydrographs up to two days in advance with continual updates. Modelling involves identifying typhoon track vectors, clustering vectors using a self-organizing map, extracting flow characteristic curves, and predicting flood hydrographs. This machine learning approach can significantly improve existing flood warning systems and provide early warnings to reservoir management.[[notice]]補正完

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field