Densidade de energia e aminoácidos em dietas de leitões na fase pré-inicial de creche: desempenho e composição química corporal

It was evaluated the effects of metabolizable energy (ME) and digestible lysine (dLYS) densities on performance and body composition of weaned piglets. The study used 114 piglets weaned at 7.4 ± 0.80 kg, out of which 108 were allotted in the nursery and 6 were slaughtered on the weaning day to determine comparative data of body chemical composition. Six nutrients densities were stipulated from a previous study based on the highest nitrogen retention, maintaining the following ME:LYS relationship in the experimental diets: 3,390:1.291; 3,450:1.409; 3,650:1.411; 3,780:1.461; 3,940:1.507; and 4,109 kcal/kg ME:1.564% dLYS. The experimental diets were offered for 13 days when the piglets reached 12.986 ± 1.449 kg of body weight. The probable residual effects of nutritional density on the subsequent performance of the piglets were evaluated. At the end of initial phase 1, six piglets from each density were slaughtered to determine their chemical composition in body fractions and empty body. There was no significant influence of nutritional levels on the performance of the piglets at the end of the evaluation. The results of food conversion and body composition confirm the level indicated in the previous study, 4 g dLYS/Mcal of ME. The increase of energy and lysine densities confirms the need for a correct relationship among both of them to assure better performance of the piglets at the beginning of the growing phase.Avaliaram-se os efeitos da densidade de energia metabolizável (EM) e lisina digestível (LIS) sobre o desempenho e a composição corporal de leitões após o desmame. Utilizaram-se 114 animais desmamados aos 7,4 ± 0,80 kg; desses animais, 108 foram alojados na unidade de creche e 6 foram abatidos no dia do desmame para determinação dos dados comparativos da composição química corporal. Seis densidades de nutrientes foram estipuladas a partir de estudo anterior, com base na maior retenção de nitrogênio, mantendo-se as seguintes relações EM:LIS nas dietas experimentais: 3.390:1,291; 3.450:1,409; 3.650:1,411; 3.780:1,461; 3.940:1,507; e 4.109 kcal/kg EM:1,564% LIS. As dietas experimentais foram oferecidas durante 13 dias, quando os leitões atingiram o peso de 12,986 ± 1,449 kg. Avaliaram-se os prováveis efeitos residuais da densidade nutricional no desempenho subseqüente dos leitões. Ao término da fase inicial-1, seis leitões de cada densidade foram abatidos para determinação da composição química nas frações corporais e no corpo vazio. Não houve influência significativa dos níveis nutricionais no desempenho dos leitões ao término da avaliação. Os resultados de conversão alimentar e composição corporal ratificam o nível indicado em estudo anterior, de 4 g LIS/Mcal. O aumento da densidade de energia e lisina confirma a necessidade da correta relação entre ambos para assegurar o melhor desempenho dos leitões na fase inicial de crescimento.Brasil - Fundação de Apoio à Pesquisa do Estado de São Paulo (FAPESP

Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol

The mycotoxin deoxynivalenol (DON), one of the most common food contaminants, primarily targets the gastrointestinal tract to affect animal and human health. This study was conducted to examine the protective function of glutamic acid on intestinal injury and oxidative stress caused by DON in piglets. Twenty-eight piglets were assigned randomly into 4 dietary treatments (7 pigs/treatment): 1) uncontaminated control diet (NC), 2) NC+DON at 4 mg/kg (DON), 3) NC+2% glutamic acid (GLU), and 4) NC+2% glutamic acid + DON at 4 mg/kg (DG). At day 15, 30 and 37, blood samples were collected to determine serum concentrations of CAT (catalase), T-AOC (total antioxidant capacity), H2O2 (hydrogen peroxide), NO (nitric oxide), MDA (maleic dialdehyde), DAO (diamine oxidase) and D-lactate. Intestinal morphology, and the activation of Akt/mTOR/4EBP1 signal pathway, as well as the concentrations of H2O2, MDA, and DAO in kidney, liver and small intestine, were analyzed at day 37. Results showed that DON significantly (P<0.05) induced oxidative stress in piglets, while this stress was remarkably reduced with glutamic acid supplementation according to the change of oxidative parameters in blood and tissues. Meanwhile, DON caused obvious intestinal injury from microscopic observations and permeability indicators, which was alleviated by glutamic acid supplementation. Moreover, the inhibition of DON on Akt/mTOR/4EBP1 signal pathway was reduced by glutamic acid supplementation. Collectively, these data suggest that glutamic acid may be a useful nutritional regulator for DON-induced damage manifested as oxidative stress, intestinal injury and signaling inhibition

370 Awardee Talk: Role of feed enzymes in gut health and function

Abstract Exogenous enzymes are routinely added to diets for non-ruminant animals, mainly to help enhance energy and nutrient utilization, thus contributing to efficient and sustainable production systems. Also, feed enzymes allow for effective utilization of non-traditional feedstuffs (e.g. co-products) in non-ruminant diets, with potential to mitigate feed cost. In addition to increased nutrient utilization, however, feed enzymes, through their impact on the gastrointestinal environment and microbial composition, can have a profound effect on indices of gut health and function. These effects may explain the reported reductions in medication costs and variability in animal performance and mortality rates observed with dietary enzyme supplementation. By acting on their target substrates, feed enzymes reduce the availability of non-digested substrates and in the case of carbohydrate-degrading enzymes can produce short-chain oligosaccharides with potential prebiotic effects. These changes are known to modulate the gut microbiome in favor of bacteria associated with a healthy gut, while reducing the population of pathogenic bacteria, which explains the reduced incidences of diarrhea that have been attributed to enzyme supplementation. Also, feed enzymes may reduce oxidative stress and enhance the functional capacity (e.g. maintaining barrier function) of the gastrointestinal tract. Feed enzymes by themselves may never be a single solution to mitigate gut health challenges in livestock production systems, but clearly there is a mounting body of evidence to support the role of feed enzymes in this regard. Therefore, their benefits will be maximized when used as part of an integrated solution approach for containing enteric pathogens of economic importance and eliminating the negative effects of dietary components with potential to compromise intestinal integrity.</jats:p

Nutritional significance of endogenous gut nitrogen losses in growing pigs

Experiments were conducted to establish the impact of diets designed to induce either low or high endogenous gut protein losses (ENL) on protein synthesis rates and energy expenditure in the visceral organs of growing pigs. First, conditions were established for converting lysine in practical feedstuffs into homoarginine (HA). This technique was then used to determine ENL in pigs fed casein-cornstarch (CC), barley (B), canola meal (CM) or a mixture of barley and canola meal (BCM) based diets. Apparent and true ileal amino acid digestibilities were also determined. Diets based on B, CM and BCM induced significantly more ENL than the CC-based diet. True ileal amino acid digestibilities in the BCM diet seemed more additive compared to apparent digestibilities. A preliminary study was conducted to confirm that a flooding-dose of phenylalanine 'per se' has no significant impact on the metabolic status of the pig. This flooding-dose technique was then used to measure protein synthesis rates in visceral organs of growing pigs fed either the CC- or the BCM-diet. The BCM diet increased daily protein synthesis rates in the colon but not in the small intestine. This finding suggests that the increase in ENL observed when feeding the BCM-based diet is due to reduced re-absorption of endogenous nitrogen rather than an increase in the secretion of endogenous nitrogen into the gut lumen. A further study was conducted to determine diet effect on organ mass and in vitro oxygen consumption in some visceral organs. In this study, the CC and BCM diets were evaluated as well as a BCM based diet with 30% added alfalfa meal (BCM-ALF). The results showed that in vitro oxygen consumption per g of tissue is not affected by diet type. The BCM and BCM-ALF diets increased organ mass compared to the CC diet and because of this effect, total energy expenditure in the visceral organs of BCM and BCM-ALF pigs was higher than in the CC-fed pigs. These findings suggest that increased ENL as a result of feeding a BCM diet compared to a CC based diet, is associated with increased energy expenditure in the hind gut. The latter is largely attributed to diet effects on organ size. It is suggested that further studies be conducted to evaluate relationships between ENL, size and energy expenditure in visceral organs when specific dietary components (e.g. feed enzymes, antinutritional factor etc.) are manipulated

PSIV-1 Apparent and Standardized Ileal Digestibility of Amino Acids in Dry Extruded-Expelled Soybean Meal Fed to Growing Pigs Without or With Organic Acids and Multi-Carbohydrase

Abstract Dry extruded-expelled soybean meal (DESBM) is an attractive feed ingredient for swine not only as a protein source but also as a good source of energy because of its high fat content. Apparent (AID) and standardized (SID) ileal amino acid (AA) digestibility in DESBM fed without or with organic acid (OA) and a multicarbohydrase (MC) supplementation to growing pigs were determined. Ten ileal-cannulated barrows (17.3 ± 0.4 kg BW) were randomly assigned to 1 of 5 dietary treatments in a replicated 5 × 4 incomplete Latin square design with 4 periods, each consisting of 5 d of adaptation and 2 d of ileal digesta collection. Four cornstarch-based diets with 44.4% DESBM as the only source of AA, without or with OA and MC supplementation, and a 5% casein-cornstarch diet were used. All diets contained 0.3% titanium dioxide. Data were analyzed using the MIXED model procedures of SAS, with the final model including OA, MC, and the interaction between OA and MC as the main effects. The SID of indispensable AA in DESBM fed to growing pigs were: Arg, 93.2%; His, 85.1%; Ile, 87.1%; Leu, 87.6%; Lys, 88.7%; Met, 87.8%; Phe, 88.4%; Thr, 81.4%; Trp, 73.6%; Val, 84.5%. OA supplementation decreased the AID and SID (P &amp;lt; 0.05) of Arg, Ile, Asp, and Glu, and tended (P &amp;lt; 0.10) to decrease the AID and SID of Phe, Trp, Cys, Gly, and Tyr. MC supplementation did not affect the AID and SID of AA, and no interactive effect on AA digestibility was observed between OA and MC supplementation. In conclusion, dietary supplementation of OA and MC showed no positive effects on improving AA digestibility in DESBM fed to growing pigs.</jats:p

Heat processing increased the digestibility of phosphorus in soybean expeller, canola meal, and canola expeller fed to growing pigs

Abstract It is hypothesized that heat processing may increase P digestibility in different protein sources fed to growing pigs. A study was conducted to determine the apparent total tract digestibility (ATTD) and standardized total tract digestibility (STTD) of P in soybean expeller (SBE) produced from oil extraction using dry extrusion and expelling and to investigate the effects of heat treatment on the ATTD and STTD of P in SBE, canola meal (CM), and canola expeller (CE) fed to growing pigs. Thirty-six growing barrows with an initial body weight of 19.0 ± 1.0 kg (mean ± SD) were assigned to 1 of 6 experimental diets in a completely randomized design to give 6 replicates per diet. The experimental design was a 3 × 2 factorial arrangement including three oilseed meals with or without heat treatment. The diets were formulated to contain non-autoclaved or autoclaved (at 121 °C for 60 min) SBE, CM, and CE as the sole source of P. Limestone was included in diets to maintain a Ca:total P ratio of 1.3:1 across diets. Pigs were individually housed in metabolism crates for 12 d, including 7 d for adaptation and 5 d for total collection of feces. Pigs were offered their daily ration at 2.8 times their maintenance energy requirement. Data were analyzed using the PROC MIXED of SAS. Heat treatment increased (P &amp;lt; 0.05) the ATTD and STTD of P. Pigs fed the SBE diets had greater (P &amp;lt; 0.05) ATTD and STTD of P than pigs fed CM and CE diets. For the autoclaved ingredients, the values of STTD of P were 49.4%, 23.2%, and 25.8% for SBE, CM, and CE, respectively, whereas STTD of P in non-autoclaved SBE, CM, and CE were 48.5%, 20.2%, and 22.5%. Heat treatment increased (P &amp;lt; 0.05) the ATTD of Ca. In conclusion, heat treatment increased ATTD and STTD of P and ATTD of Ca in SBE, CM, and CE fed to growing pigs. The ATTD and STTD of P in SBE determined in the current study were 41.0% and 48.5%, respectively.</jats:p

139 Digestibility of phosphorus in heat-treated dry extruded-expelled soybean meal and solvent-extracted and expeller-extracted canola meal fed to growing pigs

Abstract Although heat treatment affects digestibility of amino acids in feed ingredients, there is less information as to whether such an effect also applies to minerals. Therefore, the objective of this research was to investigate the effects of heat treatment on apparent (ATTD) and standardized total tract digestibility (STTD) of P in dry extruded-expelled soybean meal (DESBM), solvent-extracted canola meal (SCM), and expeller-extracted canola meal (ECM) fed to growing pigs. A total of 36 pigs (19.0 ± 1.0 kg) were assigned to 1 of 6 diets in a completely randomized design to give 6 replicates per diet. Pigs were individually housed in metabolism crates for 12 d, including 7 d for adaptation and 5 d for total collection of feces. The experimental diets were formulated to contain non-heated or heat-treated DESBM, SCM, and ECM as the sole source of P. Pigs were offered their daily ration at 2.8 times their maintenance energy requirement. Data were analyzed as a 3 × 2 factorial arrangement using the PROC MIXED of SAS with ingredients, heat treatment, and their interaction; however, no interaction was observed for any variable. Pigs fed the DESBM diet had a higher (P &amp;lt; 0.05) ATTD and STTD of P than pigs fed SCM and ECM diets. The ATTD and STTD of P in heat treated samples were higher (P &amp;lt; 0.05) than in non-heated samples. In the heat-treated samples, the values of STTD of P were 49.4, 23.2, and 25.8% for DESBM, SCM, and ECM, respectively. Respective values for STTD of P in non-heated DESBM, SCM, and ECM were 48.5, 20.2, and 22.5%. The ATTD of Ca in non-heat-treated samples was lower (P &amp;lt; 0.05) compared to the values for heat-treated samples. In conclusion, heat treatment increased ATTD and STTD of P in DESBM, SCM, and ECM fed to growing pigs.</jats:p

Effect of oat particle size on energy and nutrient utilization in growing pigs

Abstract An experiment was conducted to determine the energy content of oats and to investigate the effects of oat particle size on nutrient and energy balance in growing pigs. Eighteen barrows (23.56 ± 0.94 kg initial body weight) were randomly assigned to one of the three dietary treatments with six replicates per treatment. Whole oats were ground with a hammermill fitted with 4.8- and 3.2-mm screens to make coarse and medium particle size oats, respectively. Medium oats were further ground with a rotary steel cutting grinder fitted with a 2.0-mm screen, and the further ground oats were mixed with medium oats in a 1:3 ratio to make fine oats. Three experimental diets consisted of 96.3% of the coarse, medium, or fine oats as a sole source of energy were used. Pigs were fed diets for 16 d, including 10 d for adaptation and 6 d for total fecal and urine collection. Pigs were then moved into indirect calorimetry chambers to determine 24-h heat production and 12-h fasting heat production. All data were analyzed using the MIXED procedure of SAS with the individual pig as the experimental unit. The geometric mean particle sizes for coarse, medium, and fine oats were 765, 619, and 569 μm, respectively. Pigs fed the medium oats diet tended to have (P &amp;lt; 0.10) greater apparent total tract digestibility (ATTD) of starch, neutral detergent fiber, and gross energy than those fed coarse oats diet. The medium oats diet contained greater (P &amp;lt; 0.05) digestible energy (DE), metabolizable energy (ME), and net energy (NE) than the coarse oats diet. Pigs fed the fine oats diet had lower (P &amp;lt; 0.05) ATTD of Ca and P than those fed the coarse oats diet. The DE, ME, and NE contents of fine oats were comparable with those of coarse oats. The determined NE contents for coarse, medium, and fine oats were 2,335, 2,615, and 2,521 kcal/kg on a dry matter basis, respectively. The NE content in medium oats was greater (P &amp;lt; 0.05) than the NE values predicted using published equations. In conclusion, it was suggested to grind whole oats for 619 μm concerning energy utilization. Further grinding to 569 μm reduces Ca and P digestibility.</jats:p