77 research outputs found
Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression.A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. thermophila is described, validated and used to study gene expression during the three major stages of the organism's life cycle: growth, starvation and conjugation.Of the approximately 27,000 predicted open reading frames, transcripts homologous to only approximately 5900 are not detectable in any of these life cycle stages, indicating that this single-celled organism does indeed contain a large number of functional genes. Transcripts from over 5000 predicted genes are expressed at levels >5x corrected background and 95 genes are expressed at >250x corrected background in all stages. Transcripts homologous to 91 predicted genes are specifically expressed and 155 more are highly up-regulated in growing cells, while 90 are specifically expressed and 616 are up-regulated during starvation. Strikingly, transcripts homologous to 1068 predicted genes are specifically expressed and 1753 are significantly up-regulated during conjugation. The patterns of gene expression during conjugation correlate well with the developmental stages of meiosis, nuclear differentiation and DNA elimination. The relationship between gene expression and chromosome fragmentation is analyzed. Genes encoding proteins known to interact or to function in complexes show similar expression patterns, indicating that co-ordinate expression with putative genes of known function can identify genes with related functions. New candidate genes associated with the RNAi-like process of DNA elimination and with meiosis are identified and the late stages of conjugation are shown to be characterized by specific expression of an unexpectedly large and diverse number of genes not involved in nuclear functions
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Summary of the laser working group
The laser working group considered several options to deliver synchronized laser pulses of the required energy to the photocathode and laser triggered switches. These requirements actually decreased during the course of the workshop, and the values finally settled upon (<10 {mu}J in 100 fs at {approximately}250 nm for the photocathode and {approximately}20 mJ in 2 ps near either 250 nm or 1 {mu}m for the switches) were considered to be well within the state of the art. Some development work may be required, however, to provide a system that has the desirable characteristics of stability, ease of use and low maintenance. The baseline concept, which is similar to a number of existing systems, utilizes doubled Nd:YAG-pumped dye oscillator/amplifiers to produce an upconverted picosecond pulse that can be amplified to tens of mJ in a KrF excimer laser. A fraction of the dye oscillator output is also compressed by means of a fiber-grating compressor and further amplified in a dye amplifier before being upconverted to produce the synchronized pulse for the photocathode. 9 refs., 1 fig
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Glass Furnace Combustion and Melting Research Facility.
The need for a Combustion and Melting Research Facility focused on the solution of glass manufacturing problems common to all segments of the glass industry was given high priority in the earliest version of the Glass Industry Technology Roadmap (Eisenhauer et al., 1997). Visteon Glass Systems and, later, PPG Industries proposed to meet this requirement, in partnership with the DOE/OIT Glass Program and Sandia National Laboratories, by designing and building a research furnace equipped with state-of-the-art diagnostics in the DOE Combustion Research Facility located at the Sandia site in Livermore, CA. Input on the configuration and objectives of the facility was sought from the entire industry by a variety of routes: (1) through a survey distributed to industry leaders by GMIC, (2) by conducting an open workshop following the OIT Glass Industry Project Review in September 1999, (3) from discussions with numerous glass engineers, scientists, and executives, and (4) during visits to glass manufacturing plants and research centers. The recommendations from industry were that the melting tank be made large enough to reproduce the essential processes and features of industrial furnaces yet flexible enough to be operated in as many as possible of the configurations found in industry as well as in ways never before attempted in practice. Realization of these objectives, while still providing access to the glass bath and combustion space for optical diagnostics and measurements using conventional probes, was the principal challenge in the development of the tank furnace design. The present report describes a facility having the requirements identified as important by members of the glass industry and equipped to do the work that the industry recommended should be the focus of research. The intent is that the laboratory would be available to U.S. glass manufacturers for collaboration with Sandia scientists and engineers on both precompetitive basic research and the solution of proprietary glass production problems. As a consequence of the substantial increase in scale and scope of the initial furnace concept in response to industry recommendations, constraints on funding of industrial programs by DOE, and reorientation of the Department's priorities, the OIT Glass Program is unable to provide the support for construction of such a facility. However, it is the present investigators' hope that a group of industry partners will emerge to carry the project forward, taking advantage of the detailed furnace design presented in this report. The engineering, including complete construction drawings, bill of materials, and equipment specifications, is complete. The project is ready to begin construction as soon as the quotations are updated. The design of the research melter closely follows the most advanced industrial practice, firing by natural gas with oxygen. The melting area is 13 ft x 6 ft, with a glass depth of 3 ft and an average height in the combustion space of 3 ft. The maximum pull rate is 25 tons/day, ranging from 100% batch to 100% cullet, continuously fed, with variable batch composition, particle size distribution, and raft configuration. The tank is equipped with bubblers to control glass circulation. The furnace can be fired in three modes: (1) using a single large burner mounted on the front wall, (2) by six burners in a staggered/opposed arrangement, three in each breast wall, and (3) by down-fired burners mounted in the crown in any combination with the front wall or breast-wall-mounted burners. Horizontal slots are provided between the tank blocks and tuck stones and between the breast wall and skewback blocks, running the entire length of the furnace on both sides, to permit access to the combustion space and the surface of the glass for optical measurements and sampling probes. Vertical slots in the breast walls provide additional access for measurements and sampling. The furnace and tank are to be fully instrumented with standard measuring equipment, such as flow meters, thermocouples, continuous gas composition analyzers, optical pyrometers, and a video camera. The output from the instruments is to be continuously recorded and simultaneously made available to other researchers via the Internet. A unique aspect of the research facility would be its access to the expertise in optical measurements in flames and high temperature reacting flows residing in the Sandia Combustion Research Facility. Development of new techniques for monitoring and control of glass melting would be a major focus of the work. The lab would be equipped with conventional and laser light sources and detectors for optical measurements of gas temperature, velocity, and gaseous species and, using new techniques to be developed in the Research Facility itself, glass temperature and glass composition
Taylor Fever Thermometer
1x5x2". Body temperature:Thermometer. Original yellow paper box that reads "Taylor 'INSTANTA' Fever Thermometer" and contains a straight glass mercury thermometer, a black plastic case and a certificate of accuracy dated for October 8. 1946
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The lepton asymmetry in W production and decay at CDF
The SCF detector was used to measure the asymmetry, A(Y{sub 1}), of leptons from the decay of W bosons produced in p{bar p} collisions at {radical}{bar s} = 1.8. The W bosons were identified by the existence of an electron or muon with large transverse energy along with a large amount of missing transverse energy in the event. The observed asymmetry is the convolution of the W production asymmetry and the decay asymmetry. The production asymmetry is dependent on the d/u ratio in the proton and therefore dependent on the structure functions. The measured asymmetry is compared to predictions from various structure functions. 5 refs., 1 fig
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Coherence effects in radiometry and in spectroscopy
Some recent researches are described concerning the effects of the state of coherence of a source on the spatial and the spectral distributions of energy generated by the source. The researches have elucidated the foundations of radiometry and they have also revealed some unexpected new phenomena relating to spectral changes which can be induced by source correlations and also by scattering on random media
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