ChromatoShiny: an interactive R/Shiny App for plotting chromatography profiles [version 2; peer review: 2 approved]

BackgroundUnicorn™ software on Äkta liquid chromatography instruments outputs chromatography profiles of purified biological macromolecules. While the plots generated by the instrument software are very helpful to inspect basic chromatogram properties, they lack a range of useful annotation, customization and export options.MethodsWe use the R Shiny framework to build an interactive app that facilitates the interpretation of chromatograms and the generation of figures for publications.ResultsThe app allows users to fit a baseline, to highlight selected fractions and elution volumes inside or under the plot (e.g. those used for downstream biochemical/biophysical/structural analysis) and to zoom into the plot. The app is freely available at https://ChromatoShiny.bio.ed.ac.uk.ConclusionsIt requires no programming experience, so we anticipate that it will enable chromatography users to create informative, annotated chromatogram plots quickly and simply.FPLC instruments used to purify macromolecules output the UV intensity values over the elution volume. However, the software used with these instruments is not usually used to generate the figures for publication. To facilitate the analysis of chromatograms and generation of publication figures, we developed a web app which is possible to use without programming skills. The app is working on Äkta .txt files and is able to fit a baseline, to highlight fractions on and under the plot and to zoom into the plot. The app is designed for Äkta instruments, but the data from other softwares can be entered in the provided template and plotted accordingly. The app is well suited for plotting many similar plots. The plots can be downloaded in various formats. The app is equipped with instructions and has a user friendly interface. We hope that the app will become a helpful tool for displaying chromatograms from various FPLC softwares

Inter-professional rivalry in nigerian health sector: a search for a potential beginning

Inter-professional rivalry (IPR) in Nigerian health sector has become a commonpractice for over a decade due to abuse of the existing laws and ethics governing the operations of the professional cadres manning the health institutions in the country. This has led to incessant strikes by the different cadres in the hospitals across the country, thereby affecting the quality of services delivered by all the categories of healthcare staff. This study was aimed at identifying the initial source of the problem by interviewing the students of the Faculty of Basic Medical Sciences of Bayero University Kano (BUK) and that of Yusuf Maitama Sule University (YUMSUK) and those that have passed their examinations and joined the clinical and allied science departments in Bayero University Kano. The Faculty of Basic Medical Sciences is the initial confluent point where all medical and students of allied sciences receive their training together especially in the first and second year of their training. Method: A random sampling method was used to select the subjects. Two hundred questionnaires were distributed but 139 (69.5%) students at various levels of training filled and returned the questionnaire. Results: Females constituted 62 (44.6%), while the male were 77 (55.4%). Those in level 4 and 6 dominated the study with 55 (39.6%) and 41 (29.5%) respectively. Medical students and students of Physiology program constituted 42(30.2%) and 23(16.5%) respectively. Majority of the students 126 (90.6%) chose their respective courses without external influence and 85 (61.2%) believed that their course of study has a supportive role in the health care system. Up to 63 (45.3%) believed there is no superiority among the various courses of study. Of the factors fueling superiority feelings among students, 70 (50.4%) did not provide any reason, while those that believe professional bodies encourage it constituted 40 (28.8%), followed by teachers 11 (7.9%) and students themselves 10 (7.2%). Superiority feelings lead to rivalry among the various programs as opined by 78 (56.1%), chaos in the health sector 26 (18.7%) and poor patients management 12 (8.6%). Majority, 61 (43.9%) believed that rivalry in health sector can be prevented by giving uniform opportunity to all professional group. Conclusion: It is clear that half of the students interviewed were not aware of superiority feeling among the different programs of study. However, professional bodies rank first among the factors fueling superiority feeling during undergraduate training. Superiority feeling has negative effects on the healthcare system, one of which is rivalry among the healthcare staff and it can be avoided by giving uniform opportunity to different professional groups

Development of Web-Based Real-time Energy Monitoring System for Campus University

This study presents development of real time energy monitoring system installed in Level 17, Tower 2, Engineering Complex, Universiti Teknologi MARA (UiTM), Malaysia. The device and programming uses microcontroller board Arduino, ESP8266 Wi-Fi module, sensors and Emoncms.org web server to record and display the real time power and energy consumption, energy cost, energy index and CO2 emissions. The device allows 3 phase measurement of energy use in the building level where the main power source has been divided into several loads such as lighting, power plug, and Air Conditioning (AC) fan. The device is capable to transmit the data at set time interval using Wi-Fi communication and logging directly to local SD card for further analysis. The device was calibrated using FLUKE 485 power meter concurrently to meet a good reliable result with lower tolerance. The device was installed and tested during testing period including weekdays and weekend to see hourly energy performance in the particular building level. It is found that the device provides reading with average error of 2.04% as compared to commercial meter during one week testing period

Robot-assisted radical prostatectomy in Hong Kong: a review of 235 cases

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A comparison study between electrospun polycaprolactone and piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffolds for bone tissue engineering

This study was supported by the Federal Target Program #14.587.21.0013 (a unique application number 2015-14-588-0002-5599)

Treating rhinitis in the older population: special considerations

Rhinitis in the elderly is a common but often neglected condition. Structural changes in the nose associated with aging, predisposes the elderly to rhinitis. There are a number of specific factors that affect medical treatment of the elderly including polypharmacy, cognitive dysfunction, changes in body composition, impairment of liver and renal function and the cost of medications in the face of limited resources. Rhinitis in the elderly can be placed in several categories and treatment should be appropriate for each condition. The most important aim is to moisten the nasal mucosa since the nose of the elderly is so dry. Great caution should be used in treatment with first generation antihistamines and decongestants. Medications generally well tolerated by the elderly are second generation antihistamines, intra-nasal anti-inflammatory agents, leukotriene modifiers and iprapropium nasal spray

Topologically Protected Quantum State Transfer in a Chiral Spin Liquid

Topology plays a central role in ensuring the robustness of a wide variety of physical phenomena. Notable examples range from the robust current carrying edge states associated with the quantum Hall and the quantum spin Hall effects to proposals involving topologically protected quantum memory and quantum logic operations. Here, we propose and analyze a topologically protected channel for the transfer of quantum states between remote quantum nodes. In our approach, state transfer is mediated by the edge mode of a chiral spin liquid. We demonstrate that the proposed method is intrinsically robust to realistic imperfections associated with disorder and decoherence. Possible experimental implementations and applications to the detection and characterization of spin liquid phases are discussed.Comment: 14 pages, 7 figure