Factors affecting the fermentation process of Vietnamese traditional wine ("men la" wine) using "Ba Nang" wine starter

“Men la” wine is a traditional wine product of the upland people in Vietnam. The wine is of the cultural essence of the nation, reflecting the current trend of one village one product (OCOP). The "Ba Nang" wine starters are usually made from sticky rice and local herbs. In fact, the use of herbs in traditional wine production has become popular. However, the traditional methods used by the local producers are not standardized. The quality of the wines produced is therefore not stable and wine producers resort to laborious trials and personal experience. The aim of this study was to examine the factors affecting the fermentation process in the production of “Men la” wine using the wine starters of the Van Kieu people in Da Ban, Ba Nang commune, Dakrong district, Quang Tri province, Vietnam. The ratio of yeast to rice, solid fermentation time, temperature, and time of liquid fermentation, as well as some biochemical criteria, were investigated to determine the best parameters for "Men la" wine production. The results showed that the optimal parameters for obtaining the highest quality of wine were: a mixture ratio of wine starter and whole rice of 8g: 1000g; solid fermentation for 4 days at 30 – 32oC; and fermentation for 7 days at 25oC with a ratio of rice ingredients and water of 1:2. The final product contained 4.952% of total sugar, 0.08% of reducing sugar, 0.315% acid, 0.104 g/L amino acid, and 10.61g/100mL of ethanol. The analysis did not detect methanol in the distilled wine products. The finished wine produced on a laboratory scale had a sweet taste equivalent to that of wine, with a flavor that was thought to be superior to the local wine. The alcoholic fermentation time was shortened by 2 days compared with local wine. The resultant "Men la" wine met the National Technical Regulations for alcoholic beverage products (QCVN 6-3:2010/BYT).&nbsp

Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa

Aluminum (Al) toxicity in acid soils is a major limitation to the production of alfalfa (Medicago sativa subsp. sativa L.) in the USA. Developing Al-tolerant alfalfa cultivars is one approach to overcome this constraint. Accessions of wild diploid alfalfa (M. sativa subsp. coerulea) have been found to be a source of useful genes for Al tolerance. Previously, two genomic regions associated with Al tolerance were identified in this diploid species using restriction fragment length polymorphism (RFLP) markers and single marker analysis. This study was conducted to identify additional Al-tolerance quantitative trait loci (QTLs); to identify simple sequence repeat (SSR) markers that flank the previously identified QTLs; to map candidate genes associated with Al tolerance from other plant species; and to test for co-localization with mapped QTLs. A genetic linkage map was constructed using EST-SSR markers in a population of 130 BC(1)F(1) plants derived from the cross between Al-sensitive and Al-tolerant genotypes. Three putative QTLs on linkage groups LG I, LG II and LG III, explaining 38, 16 and 27% of the phenotypic variation, respectively, were identified. Six candidate gene markers designed from Medicago truncatula ESTs that showed homology to known Al-tolerance genes identified in other plant species were placed on the QTL map. A marker designed from a candidate gene involved in malic acid release mapped near a marginally significant QTL (LOD 2.83) on LG I. The SSR markers flanking these QTLs will be useful for transferring them to cultivated alfalfa via marker-assisted selection and for pyramiding Al tolerance QTLs

Plant Growth-Promoting Microbes from Herbal Vermicompost

Overreliance on chemical pesticides and fertilizers has resulted in problems including safety risks, outbreaks of secondary pests normally held in check by natural enemies, insecticide resistance, environmental contamination, and decrease in biodiversity. The increasing costs and negative effects of pesticides and fertilizers necessitate the idea of biological options of crop protection and production. This includes the use of animal manure, crop residues, microbial inoculum, and composts. They provide natural nutrition, reduce the use of inorganic fertilizers, develop biodiversity, increase soil biological activity, maintain soil physical properties, and improve environmental health

Assessing the vertical transmission potential of dengue virus in field-reared Aedes aegypti using patient-derived blood meals in Ho Chi Minh City, Vietnam

Background Dengue viruses (DENV) can be transmitted from an adult female Aedes aegypti mosquito through the germ line to the progeny; however, there is uncertainty if this occurs at a frequency that is epidemiologically significant. We measured vertical transmission of DENV from field-reared Ae. aegypti to their F1 progeny after feeding upon blood from dengue patients. We also examined the transmission potential of F1 females. Methods We examined the frequency of vertical transmission in field-reared mosquitoes, who fed upon blood from acutely viremic dengue patients, and the capacity for vertically infected females to subsequently transmit virus horizontally, in two sets of experiments: (i) compared vertical transmission frequency of field-reared Ae. aegypti and Ae. albopictus, in individual progeny; and (ii) in pooled progeny derived from field- and laboratory-reared Ae. aegypti. Results Of 41 DENV-infected and isofemaled females who laid eggs, only a single female (2.43%) transmitted virus to one of the F1 progeny, but this F1 female did not have detectable virus in the saliva when 14 days-old. We complemented this initial study by testing for vertical transmission in another 460 field-reared females and > 900 laboratory-reared counterparts but failed to provide any further evidence of vertical virus transmission. Conclusions In summary, these results using field-reared mosquitoes and viremic blood from dengue cases suggest that vertical transmission is uncommon. Field-based studies that build on these observations are needed to better define the contribution of vertical DENV transmission to dengue epidemiology