Not Available

Not AvailableGenetics and heterosis for quality and yield attributes in indigenous melon was studied by involving 28 cross combination obtained from crossing eight divers inbred of different intraspecific group of Cucumis melo in half- diallel fashion. The studies indicated that the additive as well as dominant component of variance were significant of most of the characters. The estimates of dominance component ($1 and$2) were higher than those of additive () component for all the characters except vine length and days to maturity. Overall evaluation indicates that parent-3, parent-6 and parent-7 were best general combiner for yield and other characters. Among the three best combination, hybrid combination P-7 x P-8 was considered best due to its maximum consumer preference in the present investigation.Not Availabl

Mechanical transmission of Tomato leaf curl New Delhi virus to cucurbit germplasm: selection of tolerance sources in Cucumis melo

[EN] Cucurbits are major crop species, including fruits and vegetables cultivated worldwide that supply essential vitamins and minerals to current diets in developed and developing countries. Viral diseases are main factors affecting cucurbits cultivation. The most widespread and damaging have been aphidborne viruses belonging to the Potyviridae family. Whitefly-transmitted begomoviruses (Geminiviridae) have been identified more recently in different cucurbit species. A severe outbreak of Tomato leaf curl New Delhi virus (ToLCNDV) occurred in pumpkins and melons in the main production area of Southern Spain in 2012 2014. We developed a mechanical inoculation method to facilitate the screening of germplasm against this virus. Mechanical transmission with this method was confirmed in 4 genera and 13 species of the family, including the main crops, cucumber, melon, watermelon and pumpkins, and also crop related exotic germplasm (landraces and wild species) used for cucurbits breeding. Diversity in the response was observed within and among species. Tolerance to mechanical transmission of ToLCNDV was identified in melon, within Cucumis melo subsp. agrestis var. momordica and in wild agrestis accessions. All the tolerant accessions came from India, the country in which this virus was firstly reported. Some of these accessions have been previously reported to be tolerant or resistant to other viruses and as they are fully crossable to commercial melons, they are good sources to develop new melon varieties with tolerance to ToLCNDV.This work was supported by Project E_RTAE2013-00020-C04-03 from the Spanish Instituto Nacional de Investigaciones Agrarias (INIA). Authors thank M. Arnedo (Ramiro Arnedo S.A.) for providing zucchini infected plants from affected greenhouses in Almeria.López Del Rincón, C.; Ferriol Molina, M.; Picó Sirvent, MB. (2015). Mechanical transmission of Tomato leaf curl New Delhi virus to cucurbit germplasm: selection of tolerance sources in Cucumis melo. Euphytica. 204:679-691. https://doi.org/10.1007/s10681-015-1371-x679691204Álvarez JM, González-Torres R, Mallor C (2005) Potential sources of resistance to Fusarium wilt and powdery mildew in melons. HortScience 40:1657–1660Bandaranayake WMEK, Wickramarachchi WART, Wickramasinghe HAM, Rajapakshe RGAS, Dissanayake DMKK (2014) Molecular detection and characterization of begomoviruses associated with cucurbitaceae vegetables in Skingri Lanka. J Natl Sci Found Sri Lanka 42:239–245Chang HH, Ku HM, Tsai WS, Chien RC, Jan FJ (2010) Identification and characterization of a mechanical transmissible begomovirus causing leaf curl on oriental melon. Eur J Plant Pathol 127:219–228Dhillon NPS, Monforte AJ, Pitrat M, Pandey S, Singh PK, Reitsma KR, Garcia-Mas J, Sharma A, McCreight JD (2012) Melon landraces of India: contributions and importance. In: Janick J (ed) Plant Breeding Rev. Wiley, Hoboken, pp 85–150Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821Fernández-Trujillo JP, Picó B, García-Mas J, Álvarez JM, Monforte AJ (2011) Breeding for fruit quality in melon. In: Jenks MA, Bebeli PJ (eds) Breeding for fruit quality. Wiley, HobokenFerriol M, Picó B (2008) Pumpkin and winter squash. In: Prohens J, Nuez F (eds) Handbook of plant breeding, vol 1., Vegetables ISpringer, Heidelberg, pp 317–349Islam S, Munshi AD, Verma M, Arya L, Mandal B, Behera TK, Kumar R, Lal SK (2010) Genetics of resistance in Luffa cylindrica Roem. against tomato leaf curl New Delhi virus. Euphytica 174:83–89Islam S, Munshi AD, Verma M, Arya L, Mandal B, Behera TK, Kumar R, Lal SK (2011) Screening of Luffa cylindrica Roem. for resistance against Tomato leaf curl New Delhi Virus, inheritance of resistance, and identification of SRAP markers linked to the single dominant resistance gene. J Hortic Sci Biotechnol 86:661–667Ito T, Sharma P, Kittipakorn K, Ikegami M (2008) Complete nucleotide sequence of a new isolate of Tomato leaf curl New Delhi virus infecting cucumber, bottle gourd and muskmelon in Thailand. Arch Virol 153:611–613Juárez M, Legua P, Mengual CM, Kassem MA, Sempere RN, Gómez P, Truniger V, Aranda MA (2013) Relative incidence, spatial distribution and genetic diversity of cucurbit viruses in eastern Spain. Ann Appl Biol 162:362–370Juárez M, Tovar R, Fiallo-Olivé E, Aranda MA, Gosálvez B, Castillo P, Moriones E, Navas-Castillo J (2014) First detection of Tomato leaf curl New Delhi virus infecting Zucchini in Spain. Plant Dis 98:857–858Jyothsna P, Haq QMI, Singh P, Sumiya KV, Praveen S, Rawat R, Briddon RW, Malathi VG (2013) Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites. Appl Microbiol Biotechnol 97:5457–5471Khan MS, Ji SH, Chun SC (2012) Begomoviruses and their emerging threats in South Korea: a review. Plant Pathol J 28:123–136King SR, Davis AR, Zhang XP, Crosby K (2010) Genetics, breeding and selection of rootstocks for Solanaceae and Cucurbitaceae. Sci Hortic 127:106–111Munera M, Giné A, Pocurull M, Picó B, Gisbert C, Sorribas FJ (2014) Comportamiento de líneas de Cucumis metuliferus, Citrullus lanatus var. citroides y C. colocynthis frente a Meloidogyne spp. Como potenciales portainjertos de melón, pepino y sandía. XVII Congreso de la Sociedad Española de Fitopatología 7–10 de octubre de 2014Navas-Castillo J, López-Moya JJ, Aranda MA (2014) Whitefly-transmitted RNA viruses that affect intensive vegetable production. Ann Appl Biol 165:155–171Papidam M, Beachy RN, Fauquet CM (1995) Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. J Gen Virol 76:25–35Paris HS (2008) Summer Squash. In: Prohens J, Nuez F (eds) Handbook of plant breeding, vol 1., Vegetables ISpringer, Heidelberg, pp 351–379Paris HS, Brown RN (2005) The genes of pumpkin and squash. HortScience 40:1620–1630Paris HS, Kabelka E (2009) Gene list for Cucurbita species, 2009. Cucurbit Genet Coop Rep 31–32:44–69Picó B, Sifres A, Nuez F (2005) Quantitative detection of cucumber vein yellowing virus in susceptible and partially resistant plants using real-time PCR. J Virol Methods 128:14–20Pitrat M (2008) Melon. In: Prohens J, Nuez F (eds) Handbook of plant breeding, vol 1., Vegetables ISpringer, Heidelberg, pp 283–315Roy A, Bal SS, Fergany M, Kaur S, Singh H, Malik AA, Singh J, Monforte AJ, Dhillon NPS (2012) Wild melon diversity in India (Punjab State). Genet Resour Crop Evol 59:755–767Samretwanich K, Chiemsombat P, Kittipakorn K, Ikegami M (2000) Tomato leaf curl geminivirus associated with cucumber yellow leaf disease in Thailand. J Phytopathol 148:615–617Sohrab SS, Karim S, Varma A, Abuzenadah AM, Chaudhary AG, Damanhouri GA, Mandal B (2013) Characterization of Tomato Leaf Curl New Delhi Virus infecting cucurbits: evidence for sap transmission in a host specific manner. Afr J Biotechnol 12:5000–5009Sohrab SS, Karim S, Varma A, Azhar EI, Abuzenadah AM, Mandal B (2014) Sap transmission of Tomato Leaf Curl New Delhi Virus infecting sponge gourd in northern India. J Plant Interact 9:241–248Srivastava KM, Hallan V, Raizada RK, Chandra G, Singh BP, Sane PV (1995) Molecular cloning of Indian tomato leaf curl virus genome following a simple method of concentrating the supercoiled replicative form of viral DNA. J Virol Methods 51:297–304Usharani KS, Surendranath B, Paul-Khurana SM, Garg ID, Malathi VG (2004) Potato leaf curl—a new disease of potato in northern India caused by a strain of Tomato leaf curl New Delhi virus. Plant Pathol 53:23

Quantitative trait loci analysis of melon (Cucumis melo L.) domestication-related traits

[EN] The fruit of wild melons is very small (20-50 g) without edible pulp, contrasting with the large size and high pulp content of cultivated melon fruits. An analysis of quantitative trait loci (QTL) controlling fruit morphology domestication-related traits was carried out using an in vitro maintained F-2 population from the cross between the Indian wild melon "Trigonus" and the western elite cultivar 'Piel de Sapo'. Twenty-seven QTL were identified in at least two out of the three field trials. Six of them were also being detected in BC1 and BC3 populations derived from the same cross. Ten of them were related to fruit morphological traits, 12 to fruit size characters, and 5 to pulp content. The Trigonus alleles decreased the value of the characters, except for the QTL at andromonoecious gene at linkage group (LG) II, and the QTL for pulp content at LGV. QTL genotypes accounted for a considerable degree of the total phenotypic variation, reaching up to 46%. Around 66% of the QTL showed additive gene action, 19% exhibited dominance, and 25% consisted of overdominance. The regions on LGIV, VI, and VIII included the QTL with more consistent and strong effects on domestication-related traits. QTLs on those regions were validated in BC2S1, BC2S2, and BC3 families, with "Trigonus" allele decreasing the fruit morphological traits in all cases. The validated QTL could represent loci involved in melon domestication, although further experiments as genomic variation studies across wild and cultivated genotypes would be necessary to confirm this hypothesis.We thank S. Casal, A. Mercader, and M. Mohamed-Amit for technical support and D. L. Goodchild for reviewing the English language. This work was supported by the Spanish Ministry of Economy and Competitiveness/FEDER grants AGL2012-40130-C02-02, AGL2015-64625-C2-2-R to AJM, AGL2014-53398-C2-2-R to BP, AGL2015-64625-C2-1-R, Centro de Excelencia Severo Ochoa 2016-2020, and the CERCA Programme/Generalitat de Catalunya to JGM and AMMM-H. AD was supported by a JAE-Doc contract from CSIC.Diaz, A.; Martin Hernandez, A.; Dolcet-Sanjuan, R.; Garcés Claver, AB.; Alvarez, J.; Garcia-Mas, J.; Picó Sirvent, MB.... (2017). Quantitative trait loci analysis of melon (Cucumis melo L.) domestication-related traits. Theoretical and Applied Genetics. 130(9):1837-1856. https://doi.org/10.1007/s00122-017-2928-yS183718561309Ashrafi H, Kinkade MP, Merk HL, Foolad MR (2012) Identification of novel quantitative trait loci for increased lycopene content and other fruit quality traits in a tomato recombinant inbred line population. Mol Breed 30:549–567Asins MJ, Breto MP, Carbonell EA (1993) Salt tolerance in Lycopersicon species. II. Genetic effects and a search for associated traits. Theor Appl Genet 86:769–774Boualem A, Fergany M, Fernandez R, Troadec C, Martin A, Morin H, Sari MA, Collin F, Flowers JM, Pitrat M, Purugganan MD, Dogimont C, Bendahmane A (2008) A conserved mutation in an ethylene biosynthesis enzyme leads to andromonoecy in melons. Science 321:836–838Brewer MT, Lang L, Fujimura K, Dujmovic N, Gray S, van der Knaap E (2006) Development of a controlled vocabulary and software application to analyze fruit shape variation in and other plant species. Plant Phys 141:15–25Capel C, Fernández del Carmen A, Alba JM, Lima-Silva V, Hernández-Gras F, Salinas M, Boronat A, Angosto T, Botella MA, Fernández R, Granell A, Capel J, Lozano R (2015) Wide-genome QTL mapping of fruit quality traits in a tomato RIL population derived from the wild-relative species Solanum pimpinellifolium L. Theor Appl Genet 128:2019–2203Cohen S, Itkin M, Yeselson Y, Tzuri G, Portnoy V, Harel-Baja R, Lev S, Sa’ar U, Davidovitz-Rikanati R, Baranes N, Bar E, Wolf D, Petreikov M, Shen S, Ben-Dor S, Rogachev I, Aharoni A, Ast T, Schuldiner M, Belausov E, Eshed R, Ophir R, Sherman A, Frei B, Neuhaus HE, Xu Y, Fei Z, Giovannoni J, Lewinsohn E, Tadmor Y, Paris HS, Katzir N, Burger Y, Schaffer AA (2014) The PH gene determines fruit acidity and contributed to the evolution of sweet melons. Nat Commun 5:4026Deleu W, Esteras C, Roig C, Gonzalez-To M, Fernandez-Silva I, Gonzalez-Ibeas D, Blanca J, Aranda MA, Arus P, Nuez F, Monforte AJ, Pico MB, Garcia-Mas J (2009) A set of EST-SNPs for map saturation and cultivar identification in melon. BMC Plant Biol 9:90Dhillon NPS, Ranjana R, Singh K, Eduardo I, Monforte AJ, Pitrat M, Dhillon NK, Singh PP (2007) Diversity among landraces of Indian snapmelon (Cucumis melo var. momordica). Genet Res Crop Evol 54:1267–1283Díaz A, Fergany M, Formisano G, Ziarsolo P, Blanca J, Fei Z, Staub JE, Zalapa JE, Cuevas HE, Dace G, Oliver M, Boissot N, Dogimont C, Pitrat M, Hofstede R, van Koert Harel-Beja R, Tzuri G, Portnoy V, Cohen S, Schaffer A, Katzir N, Xu Y, Zhang H, Fukino N, Matsumoto S, Garcia-Mas J, Monforte AJ (2011) A consensus linkage map for molecular markers and quantitative trait loci associated with economically important traits in melon (Cucumis melo L.). BMC Plant Biol 11:111Díaz A, Zarouri B, Fergany M, Eduardo I, Álvarez JM, Picó B, Monforte AJ (2014) Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘Piel de Sapo’ melon (Cucumis melo L.). PLoS One 9:e104188Díaz A, Forment J, Argyris JM, Fukino N, Tzuri G, Harel-Beja R, Katzir N, Garcia-Mas J, Monforte AJ (2015) Anchoring the consensus ICuGI genetic map to the melon (Cucumis melo L.) genome. Mol Breed 35:188Doebley JF, Gaut BS, Smith BD (2006) The molecular genetics of crop domestication. Cell 127:1309–1321Doligez A, Bertrand Y, Farnos M, Grolier M, Romieu C, Esnault F, Dias S, Berger G, Francois P, Pons T, Ortigosa P, Roux C, Houel C, Laucou V, Bacilieri R, Peros JP (2013) New stable QTLs for berry weight do not colocalize with QTLs for seed traits in cultivated grapevine (Vitis vinifera L.). BMC Plant Biol 13:217Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Eduardo I, Arús P, Monforte AJ, Obando J, Fernández-Trujillo JP, Martínez JA, Alarcón AL, Álvarez JM, van der Knaap E (2007) Estimating the genetic architecture of fruit quality traits in melon using a genomic library of near isogenic lines. J Am Soc Hortic Sci 132:80–89Esteras C, Formisano G, Roig C, Díaz A, Blanca J, García-Mas J, Gómez-Gillamón ML, López-Sesse A, Lázaro A, Monforte A, Pico B (2013) SNP genotyping in melons: genetic variation, population structure and linkage disequilibrium. Theor Appl Genet 126:1285–1303FAO (2017) Statistics Division of Food and Agriculture Organization of the United Nations (FAOSTAT). http://faostat.fao.org/ . Accessed 30 May 2017Fazza AC, Dallagnol LJ, Fazza AC, Monteiro CC, Lima BM, Wassano DT, Camargo LEA (2013) Mapping of resistance genes to races 1, 3 and 5 of Podosphaera xanthii in melon PI 414723. Crop Breed Appl Biot 13:349–355Fernandez-Silva I, Moreno E, Eduardo I, Arús P, Álvarez JM, Monforte AJ (2009) On the genetic control of heterosis for fruit shape in melon (Cucumis melo L.). J Hered 100:229–235Fernandez-Silva I, Moreno E, Essafi A, Fergany M, Garcia-Mas J, Martín Hernández AM, Álvarez JM, Monforte AJ (2010) Shaping melons: agronomic and genetic characterization of QTLs that modify melon fruit morphology. Theor Appl Genet 121:931–940Frary A, Nesbitt TC, Grandillo S, van der Knaap E, Cong B, Liu JP, Meller J, Elber R, Alpert KB, Tanksley SD (2000) fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289:85–88Garcia-Mas J, Monforte AJ, Arús P (2004) Phylogenetic relationships among Cucumis species based on the ribosomal internal transcribed spacer sequence and microsatellite. Plant Syst Evol 248:191–203Garcia-Mas J, Benjak A, Sanseverino W, Bourgeois M, Mir G, Gonzalez VM, Henaff E, Camara F, Cozzuto L, Lowy E, Alioto T, Capella-Gutierrez S, Blanca J, Canizares J, Ziarsolo P, Gonzalez-Ibeas D, Rodriguez-Moreno L, Droege M, Du L, Alvarez-Tejado M, Lorente-Galdos B, Mele M, Yang L, Weng Y, Navarro A, Marques-Bonet T, Aranda MA, Nuez F, Pico B, Gabaldon T, Roma G, Guigo R, Casacuberta JM, Arus P, Puigdomenech P (2012) The genome of melon (Cucumis melo L.). Proc Nat Acad Sci 109(29):11872–11877Gonzalo MJ, Claveria E, Mofnorte AJ, Dolcet-Sanjuan R (2011) Parthenogenic haploids in melon: generation and molecular characterization of a doubled haploid line population. J Am Soc Hort Sci 136:145–154Harel-Beja R, Tzuri G, Portnoy V, Lotan-Pompan M, Lev S, Cohen S, Dai N, Yeselson L, Meir A, Libhaber SE, Avisar E, Melame T, van Koert P, Verbakel H, Hofstede R, Volpin H, Oliver M, Fougedoire A, Stalh C, Fauve J, Copes B, Fei Z, Giovannoni J, Ori N, Lewinsohn E, Sherman A, Burger J, Tadmor Y, Schaffer AA, Katzir N (2010) A genetic map of melon highly enriched with fruit quality QTL and EST markers, including sugar and carotenoid metabolism genes. Theor Appl Genet 121:511–533Kenigsbuch D, Cohen Y (1990) The inheritance of gynoecy in muskmelon. Genome 33:317–320Kirkbride JH Jr. (1993) Biosystematic monograph of the genus Cucumis (Cucurbitaceae). Parkway, BooneKosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199Leida C, Moser C, Esteras C, Sulpice R, Lunn JE, de Langen F, Monforte AJ, Pico B (2015) Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.). BMC Genet 16:28Lippman Z, Tanksley SD (2001) Dissecting the genetic pathway to extreme fruit size in tomato using a cross between the small-fruited wild species Lycopersicon pimpinellifolium and L. esculentum var. Giant Heirloom. Genetics 158:413–422Liu JP, Van Eck J, Cong B, Tanksley SD (2002) A new class of regulatory genes underlying the cause of pear-shaped tomato fruit. Proc Natl Acad Sci USA 99:13302–13306Meyer RS, Purugganan MD (2013) Evolution of crop species: genetics of domestication and diversification. Nat Rev Genet 14:840–852Monforte AJ, Asins MJ, Carbonell EA (1997) Salt tolerance in Lycopersicon species VI. Genotype-by-salinity interaction in quantitative trait loci detection: constitutive and response QTLs. Theor Appl Genet 95:706–713Monforte AJ, Eduardo I, Abad S, Arús P (2005) Inheritance mode of fruit traits in melon: heterosis for fruit shape and its correlation with genetic distance. Euphytica 144:31–38Monforte AJ, Díaz A, Caño Delgado A, van der Knaap E (2014) The genetic basis of fruit morphology in horticultural crops: lessons from tomato and melon. J Exp Bot 65:4625–4637Muños S, Ranc N, Botton E, Bérard A, Rolland S, Duffé P, Carretero Y, Le Paslier MC, Delalande C, Bouzayen Brunel D, Causse M (2011) Increase in tomato locule number is controlled by two SNPs located near WUSCHEL. Plant Physiol 156:2244–2254Nerson H, Paris HS (2000) Relationship between fruit size and seed size in cucurbits. Cucurbit Genet Coop Rept 23:64–67Obando J, Fernández-Trujillo JP, Martínez JA, Alarcón AL, Eduardo I, Arús P, Monforte AJ (2008) Identification of melon fruit quality quantitative trait loci using near-isogenic lines. J Am Soc Hortic Sci 133:139–151Olsen KM, Wendel JF (2013) A bountiful harvest: genomic insights into crop domestication phenotypes. Annu Rev Plant Biol 64:47–70Paris MK, Zalapa JE, McCreight JD, Staub JE (2008) Genetic dissection of fruit quality components in melon (Cucumis melo L.) using a RIL population derived from exotic × elite US Western Shipping germplasm. Mol Breed 22:405–419Périn C, Hagen LS, Giovinazzo N, Besombes D, Dogimont C, Pitrat M (2002) Genetic control of fruit shape acts prior to anthesis in melon (Cucumis melo L.). Mol Genet Genom 266:933–941Perpiñá G, Esteras C, Gibon Y, Monforte AJ, Picó B (2016) A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits. BMC Plant Biol 16:154Pitrat M (2012) Domestication and diversification of melon. In: Sari N, Solmaz I, Aras V (eds) Cucurbitaceae 2012, Proceedings of Xth Eucarpia meeting, Antaley (Turkey), pp 31–39Pitrat M (2013) Phenotypic diversity in wild and cultivated melons (Cucumis melo). Plant Biotechnol 30:273–278Pitrat M (2017) Melon genetic resources: phenotypic diversity and horticultural taxonomy. In: Grumet R, Katzir N, Garcia-Mas J (eds) Genetics and genomics of the Cucurbitaceae. Springer, New York. doi: 10.1007/7397_2017_1Qi J, Liu X, Shen D, Miao H, Xie B, Li X, Zeng P, Wang S, Shang Y, Gu X, Du Y, Li Y, Lin T, Yuan J, Yang X, Chen J, Chen H, Xiong X, Huang K, Fei Z, Mao L, Tian L, Städler T, Renner SS, Kamoun S, Lucas WJ, Zhang Z, Huang S (2013) A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity. Nat Genet 45:1510–1515Ramamurthy RK, Waters BM (2015) Identification of fruit quality and morphology QTLs in melon (Cucumis melo) using a population derived from flexuosus and cantalupensis botanical groups. Euphytica 204:163–177Roy A, Bal SS, Fergany M, Kaur S, Singh H, Malik AA, Singh J, Monforte AJ, Dhillon NPS (2012) Wild melon diversity in India (Punjab State). Genet Resour Crop Evol 59:755–767Sabato D, Esteras C, Grillo O, Pico B, Bacchetta G (2015) Seeds morpho-colourimetric analysis as complementary method to molecular characterization of melon diversity. Sci Hortic-Amsterdam 192:441–452Sebastian P, Schaefer H, Telford IRH, Renner SS (2010) Cucumber (Cucumis sativus) and melon (C. melo) have numerous wild relatives in Asia and Australia, and the sister species of melon is from Australia. Proc Natl Acad Sci USA 107:14269–14273Soller M, Brody T (1976) On the power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theor Appl Genet 47:35–39Tanksley SD, Nelson JC (1996) Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet 92:191–203Voorrips RE (2002) MapChart software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78Wang S, Basten CJ, Zeng ZB (2007) Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, RaleighYang J, Hu CC, Hu H, Yu RD, Xia Z, Ye XZ, Zhu J (2008) QTLNetwork: mapping and visualizing genetic architecture of complex traits in experimental populations. Bioinformatics 24:721–723Zalapa JE, Staub JE, McCreight JD, Chung SM, Cuevas H (2007) Detection of QTL for yield-related traits using recombinant inbred lines derived from exotic and elite US Western Shipping melon germplasm. Theor Appl Genet 114:1185–1201Zeng ZB (1993) Theoretical basis of separation of multiple linked gene effects on mapping quantitative trait loci. Proc Natl Acad Sci USA 90:10972–1097