Quantitative Analysis of Candida Cell Wall Components by Flow Cytometrywith Triple-Fluorescence Staining

This work was supported by the European Commission within the FP7 Framework Programme [Fungitect-Grant No 602125]. We also thank Thomas Sauer, Vienna Biocenter Campus (VBC), Austria, for technical support at the FACS facility of the MFPL, Karl Kuchler, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Max F. Perutz Laboratories, Campus Vienna Biocenter, Vienna, Austria and Ernst Thuer, Centre for Genomic Regulation, Barcelona, Spain, for advice on statistical approaches. Neil Gow acknowledges the support of the Wellcome Trust and the MRC Centre for Medical MycologyPeer reviewedPublisher PD

Site-selective protein modification via disulfide rebridging for fast tetrazine/trans-cyclooctene bioconjugation

An inverse electron demand Diels–Alder reaction between tetrazine and trans-cyclooctene (TCO) holds great promise for protein modification and manipulation. Herein, we report the design and synthesis of a tetrazine-based disulfide rebridging reagent, which allows the site-selective installation of a tetrazine group into disulfide-containing peptides and proteins such as the hormone somatostatin (SST) and the antigen binding fragment (Fab) of human immunoglobulin G (IgG). The fast and efficient conjugation of the tetrazine modified proteins with three different TCO-containing substrates to form a set of bioconjugates in a site-selective manner was successfully demonstrated for the first time. Homogeneous, well-defined bioconjugates were obtained underlining the great potential of our method for fast bioconjugation in emerging protein therapeutics. The formed bioconjugates were stable against glutathione and in serum, and they maintained their secondary structure. With this work, we broaden the scope of tetrazine chemistry for site-selective protein modification to prepare well-defined SST and Fab conjugates with preserved structures and good stability under biologically relevant conditions

Resolved Young Binary Systems And Their Disks

We have conducted a survey of young single and multiple systems in the Taurus–Auriga star-forming region with the Atacama Large Millimeter Array (ALMA), substantially improving both the spatial resolution and sensitivity with which individual protoplanetary disks in these systems have been observed. These ALMA observations can resolve binary separations as small as 25–30 au and have an average 3σ detection level of 0.35 mJy, equivalent to a disk mass of 4 × 10−5 M ⊙ for an M3 star. Our sample was constructed from stars that have an infrared excess and/or signs of accretion and have been classified as Class II. For the binary and higher-order multiple systems observed, we detect λ = 1.3 mm continuum emission from one or more stars in all of our target systems. Combined with previous surveys of Taurus, our 21 new detections increase the fraction of millimeter-detected disks to over 75% in all categories of stars (singles, primaries, and companions) earlier than spectral type M6 in the Class II sample. Given the wealth of other information available for these stars, this has allowed us to study the impact of multiplicity with a much larger sample. While millimeter flux and disk mass are related to stellar mass as seen in previous studies, we find that both primary and secondary stars in binary systems with separations of 30–4200 au have lower values of millimeter flux as a function of stellar mass than single stars. We also find that for these systems, the circumstellar disk around the primary star does not dominate the total disk mass in the system and contains on average 62% of the total mass

Resolved Young Binary Systems And Their Disks

We have conducted a survey of young single and multiple systems in the Taurus–Auriga star-forming region with the Atacama Large Millimeter Array (ALMA), substantially improving both the spatial resolution and sensitivity with which individual protoplanetary disks in these systems have been observed. These ALMA observations can resolve binary separations as small as 25–30 au and have an average 3σ detection level of 0.35 mJy, equivalent to a disk mass of 4 × 10−5 M ⊙ for an M3 star. Our sample was constructed from stars that have an infrared excess and/or signs of accretion and have been classified as Class II. For the binary and higher-order multiple systems observed, we detect λ = 1.3 mm continuum emission from one or more stars in all of our target systems. Combined with previous surveys of Taurus, our 21 new detections increase the fraction of millimeter-detected disks to over 75% in all categories of stars (singles, primaries, and companions) earlier than spectral type M6 in the Class II sample. Given the wealth of other information available for these stars, this has allowed us to study the impact of multiplicity with a much larger sample. While millimeter flux and disk mass are related to stellar mass as seen in previous studies, we find that both primary and secondary stars in binary systems with separations of 30–4200 au have lower values of millimeter flux as a function of stellar mass than single stars. We also find that for these systems, the circumstellar disk around the primary star does not dominate the total disk mass in the system and contains on average 62% of the total mass

N=2-Maxwell-Chern-Simons model with anomalous magnetic moment coupling via dimensional reduction

An N=1--supersymmetric version of the Cremmer-Scherk-Kalb-Ramond model with non-minimal coupling to matter is built up both in terms of superfields and in a component-field formalism. By adopting a dimensional reduction procedure, the N=2--D=3 counterpart of the model comes out, with two main features: a genuine (diagonal) Chern-Simons term and an anomalous magnetic moment coupling between matter and the gauge potential.Comment: 15 pages, Latex; one reference corrected; To be published in the Int. J. Mod. Phys.

Gauge dependenceof the order parameter anomalous dimension in the Ginzburg-Landau model and the critical fluctuations in superconductors

The critical fluctuations of superconductors are discussed in a fixed dimension scaling suited to describe the type II regime. The gauge dependence of the anomalous dimension of the scalar field is stablished exactly from the Ward-Takahashi identities. Its fixed point value gives the $\eta$ critical exponent and it is shown that $\eta$ is gauge independent, as expected on physical grounds. In the scaling considered, $\eta$ is found to be zero at 1-loop order, while $\nu\approx 0.63$. This result is just the 1-loop values for the XY model obtained in the fixed dimension renormalization group approach. It is shown that this XY behavior holds at all orders. The result $\eta=\eta_{XY}$ should be contrasted with the negative values frequently reported in the literature.Comment: EuroLaTex, 7 pages, 2 figures, reference updated; version to be published in Europhysics Letter

Secagem forçada em sementes de Podocarpus lambertii e Podocarpus selowii.

bitstream/CNPF-2009-09/39420/1/com_tec140.pd

Self-dual vortices in a Maxwell-Chern-Simons model with non-minimal coupling

We find self-dual vortex solutions in a Maxwell-Chern-Simons model with anomalous magnetic moment. From a recently developed N=2-supersymmetric extension, we obtain the proper Bogomol'nyi equations together with a Higgs potential allowing both topological and non-topological phases in the theory.Comment: 12 pages, 9 figures, 2 tables; some typos corrected, one reference updated. To be published in the Int. J. Mod. Phys. A (1999