15 research outputs found
Effect of medium composition on the production of anthocyanins by hairy root cultures of Ipomoea batatas
Effect of repeated harvesting on the content of caffeic acid and seven species of caffeoylquinic acids in sweet potato leaves
Simple and Rapid Spectrophotometric Method for Selecting Purple-Fleshed Sweet Potato Cultivars with a High Radical-Scavenging Activity
Lipoxygenase activities during development of root and nodule of soybean Atividade de lipoxigenases durante o desenvolvimento da raiz e do nódulo de plantas de soja
The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.<br>O objetivo deste trabalho foi avaliar lipoxigenases de raízes e nódulos de plantas de soja da variedade Doko e da linhagem triplo nula derivada, desprovida das lipoxigenases da semente, com e sem inoculação de Bradyrhizobium elkanii. A atividade de lipoxigenase foi avaliada em raízes coletadas aos 3, 5, 9, 13, 18 e 28 dias após a inoculação e em nódulos coletados aos 13, 18 e 28 dias após a inoculação. Os perfis de pH-atividade de raiz e nódulo sugerem que o "pool" de lipoxigenases expresso nesses órgãos não difere nos dois genótipos. A atividade de lipoxigenases nas raízes de Doko e Doko triplo nula, com e sem inoculação, declinou com o passar do tempo. A maior atividade de lipoxigenases no início de formação da raiz sugere o envolvimento desta enzima no crescimento e desenvolvimento deste órgão. Nos nódulos houve um aumento acentuado na atividade de lipoxigenase aos 28 dias após a inoculação. Dois grupos de mobilidade com aproximadamente 94 e 97 kDa foram encontrados nos "immunoblottings" para lipoxigenases de raiz e de nódulo