Local intra-uterine Ang-(1-7) infusion attenuates PGE2 and 6-keto PGF1α in decidualized uterus of pseudopregnant rats

Background: Cyclooxygenase (COX)-derived prostanoids (PGE2, PGI2) are important contributors to the process of decidualization. Previous studies showed the presence of Ang-(1-7) in the primary and secondary decidualized zones of the implantation site at early pregnancy. Decreased concentrations of Ang-(1-7) were found in the decidualized uterus compared to the non-decidualized uterus of pseudopregnant rats, suggesting that low levels of Ang-(1-7) are required for successful decidualization at early pregnancy. Methods: To understand the role of Ang-(1-7) in prostaglandin production in a decidualized uterus, induced by a bolus injection of sesame oil, Ang-(1-7) (24 μg/kg/h) or vehicle was then infused directly into the decidualized uterine horn using an osmotic minipump. The right horns were not injected or infused and served as nondecidualized uterine horns in both groups of animals. Results: Decidualization increased PGE2 concentration in the uterus (0.53±0.05 vs. 12.0±3.2 pmol/mg protein, p\u3c0.001, non-decidualized vs. decidualized horns); Ang-(1-7) infusion attenuated the increase of PGE2 (12.0± 3.2 vs. 5.1±1.3 pmol/mg protein, p\u3c0.01 control vs. Ang-(1-7) treated decidualized horns). The stable metabolite of PGI2 (6-keto PGF1α) was increased with decidualization (0.79±0.17 vs. 3.5±0.82 pmol/mg protein, p\u3c0.001, non-decidualized vs. decidualized horns). Ang-(1-7) infusion attenuated the increase in 6keto PGF1α in the decidualized horn (3.5±0.82 vs 1.8±0.37 pmol/mg protein, p\u3c0.05 control vs. Ang-(1-7) treated decidualized horns). The circulating levels of 6-keto-PGF1a and TXB2 were decreased by Ang-(1-7) infusion, while no difference was observed in circulating PGE2. Although the global assessment of cleaved caspase 3 immunostaining, a marker of apoptosis, was unchanged within the Ang-(1-7) decidualized horn, there were localized decreases in cleaved caspase 3 staining in the luminal region in the decidualized uterus of Ang-(1-7)-treated rats. Conclusions: These studies show that increased local uterine Ang-(1-7) alters the uterine prostaglandin environment, possibly leading to disruptions of early events of decidualization

A canonical transformation and the tunneling probability for the birth of an asymptotically DeSitter universe with dust

In the present work, we study the quantum cosmology description of closed Friedmann-Robertson-Walker models in the presence of a positive cosmological constant and a generic perfect fluid. We work in the Schutz's variational formalism. If one uses the scale factor and its canonically conjugated momentum as the phase space variables that describe the geometrical sector of these models, one obtains Wheeler-DeWitt equations with operator ordering ambiguities. In order to avoid those ambiguities and simplify the quantum treatment of the models, we introduce new phase space variables. We explicitly demonstrate that the transformation leading from the old set of variables to the new one is canonical. In order to show that the above canonical transformations simplify the quantum treatment of those models, we consider a particular model where the perfect fluid is dust. We solve the Wheeler-DeWitt equation numerically using the Crank-Nicholson scheme and determine the time evolution of the initial wave function. Finally, we compare the results for the present model with the ones for another model where the only difference is the presence of a radiative perfect fluid, instead of dust.Comment: Revtex4, 18 pages, 2 EPS figure

Local uterine Ang-(1-7) infusion augments the expression of cannabinoid receptors and differentially alters endocannabinoid metabolizing enzymes in the decidualized uterus of pseudopregnant rats

Background Endocannabinoids (ECs) are important contributors to implantation and decidualization and are suppressed in early pregnancy. Elevated levels of anandamide (AEA), the endogenous ligand for the CB1 and CB2 receptors (R), interfere with receptivity of the blastocyst. Ang-(1–7) is down-regulated in the implantation site (IS) in normal pregnancy at day 7 of gestation. We determined the effects of intra-uterine angiotensin-(1–7) [Ang-(1–7)] (24 microg/kg/h) or vehicle given into the left uterine horn on the ECs in the decidualized uterus. Methods Ovariectomized rats were sensitized for the decidual cell reaction by steroid treatment and decidualization was induced by a bolus of oil injected into the left horn; the right horn served as a control. Results Decidualization increased endometrial permeability (3.1+/−0.2 vs. 7.1+/−0.5 uterus/muscle of cpm of (125)I-BSA, p \u3c 0.0001). VEGF mRNA was increased by the decidualization (1.4-fold, p \u3c 0.05) and by Ang-(1–7) (2.0-fold, p \u3c 0.001). CB1R mRNA was reduced by decidualization (2.7-fold, p \u3c 0.001), but increased by Ang-(1–7) (1.9-fold, p \u3c 0.05). CB2R mRNA was increased by decidualization (4-fold, p \u3c 0.05) and by Ang-(1–7) (2.4-fold, p \u3c 0.001). The enzyme metabolizing AEA, fatty acid amide hydrolase (FAAH), was reduced by decidualization (7.8 fold, p \u3c 0.001) and unchanged by Ang-(1–7) (p \u3e 0.05), whereas the enzyme metabolizing 2-arachidonoylglycerol, monoacyl glycerol lipase (MAGL), was unchanged by decidualization (p \u3e 0.05) and increased by Ang-(1–7) (1.7 fold, p \u3c 0.001). Conclusions These findings report for the first time that Ang-(1–7) augments the expression of CB1R, CB2R and MAGL in the decidualized uterus and thus may interfere with the early events of decidualization

Novel PHB/PCL scaffolds produced by melt base technologies

[Excerpt] On this work, the natural origin polymer Polyhydroxybutyrate (PHB) was melt blended with synthetic aliphatic polyester Polye-caprolactone (PCL). The ratio of PHB/PCL was varied from 25% to 75% by weight. These blends were further injection moulded and characterized. The thermal properties of the developed blends were accessed by differential scanning calorimetry DSC). The effect of the ratio PHB/PCL on the mechanical properties (tensile modulus and tensile strength) was determined by tensile tests. […]info:eu-repo/semantics/publishedVersio

Características relativas ao crescimento em meio de cultura e a morfologia de colônias de "rizóbio".

Caracterização morfológica dos isolados. Tempo de crescimento. Alteração no pH do meio de cultura. Diâmetro das colônias. Transparência e forma das colônias. Superfície das colônias. Produção, consistência e aparência do muco.bitstream/CNPAB-2010/27144/1/cot019.pd

Properties of melt processed chitosan and aliphatic polyester blends

The activities (at pH 7 and 50°C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that the presence of cellulose binding domains (CBDs) is not essential for cellulase performance in the textile processes, where high levels of mechanical agitation are applied. The binding reversibilities of these cellulases and their cores were studied by dilution of the treatment liquor after equilibrium adsorption. EGV showed low percentage of adsorption under both levels of agitation. It was observed that the adsorption/desorption processes of cellulases are enhanced by higher mechanical agitation levels and that the binding of cellulase with CBD of family I (EGV) is more reversible than that of CBD of the cellulase of family II (CenA)

Tolerância de rizóbio de feijão-caupi à salinidade e à temperatura em condição in vitro.

A seleção de estirpes de rizóbio tolerantes a estresses de salinidade e temperatura, característicos da região semi-árida, arnplia as chances de sucesso para a introdução e estabelecimento de bactérias fixadoras do nitrogênio atmosférico em associação com o feijão-caupi, resultando no aumento da produtividade de grãos. Fatores como tolerância a diferentes concentrações de NaCl (1%,2% e 3%) e dois níveis de temperatura elevada (39°C e 42°C) foram estudados em setenta e seis estirpes de rizóbio, isoladas de nódulos de feijão-caupi, com o objetivo de identificar as relações com a região de origem e habito de crescimento em meio de cultura YEM sólido. De modo geral, observou-se que as estirpes provenientes do Sertão se mostraram mais tolerantes a temperaturas elevadas. O estudo dos efeitos da salinidade (NaCl), por outro lado, mostrou que a medida que a concentração do sal aumenta ha uma redução na porcentagem de estirpes tolerantes, sendo que cerca de 40% delas foram capazes de crescer em meio de cultura contendo 1% de NaCl. Na concentração de 2% de NaCl, 17% do total foram capazes de crescer, sendo que 15% eram provenientes do Sertão e 24% da Zona da Mata. Entretanto, na concentração de 3% de NaCl, apenas 12% das estirpes testadas foram capazes de crescer. Cerca de 10% das setenta e seis estirpes mostraram-se tolerantes as altas temperaturas e aos níveis de NaCl testados, sendo uma oriunda do Agreste, quatro do Sertão e duas da Zona da Mata. The selection of rhizobium strains tolerant to salinity and temperature stress, typically of semi-arid regions, enhances the chance for introducing and establishing the air nitrogen fixing bacteria and cowpea association successfully, resulting in grain yield increases. Effects of factors, such as tolerance to different NaCI concentrations (1%, 2% and 3%) and two levels of high temperature (390 C and 420 C). were studied on seventy-six rhizobium strains isolated from cowpea root nodules in order to identify the relation to the origin region and their growth habit in YEM solid culture medium. In general, it was observed that bacteria from the "Sertão" region were more tolerant to high temperatures. On the other hand, results for the salinity study showed that as the salt concentration increased, the percentage of tolerant strains decreased. About 40% of the strains were able to grow in a culture medium with 1% of NaCl. At 2% of NaCl concentration, 17% of the total strains could grow and, from this amount, 15% came from the "Sertão" region and 24% from "Zona da Mata" region. At 3% NaCI concentration, only 12% of the strains were able to grow. From the 76 strains, around 10% were tolerant to high temperatures and to the NaCl levels used, one of them from the "Agreste" region, four from the "Sertão" resigno and 2 from the "Zona da Mata" region".Parcerias: UNEB