23 research outputs found

    Identificação dos coleópteros (insecta: Coleoptera) das regiões de Palmas (município de Bagé) e Santa Barbinha (município de Caçapava do Sul, RS).

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    Classe Insecta; Generalidades; Ordem Coleoptera; Características gerais; Material e métodos; Descrição das áreas; Técnica de amostragem; Resultados e discussão; Análise das famílias da ordem Coleoptera a partir de níveis tráfico; Raridade de famílias e ou espécies; Descrição dos táxons coletados, famílias Anobiidae, Anthicidae, Aphodiidae, Buprestidae, Cantharidae, Carabidae, Cerambycidae, Chelonariidae, Chrysomelidae, Cleridae, Coccinelidae, Corylophidae, Curculionidae, Dryopidae, Dynastidae, Elateridae, Endomychidae, Erotylidae, Geotrupidae, Histeridae, Hybosoridae, Hydrophilidae, Lampyridae, Lathridiidae, Leiodidae, Limnichidae, Meloidae, Melolonthidae, Monotomidae, Mordellidae, Nitidulidae, Phalacridae, Ptillidae, Rutelidae, Scarabaeidae, Scydmaenidae, Silvanidae, Staphylinidae, Tenebrionidae, Trogidae.bitstream/item/63866/1/DT-70.pd

    Ecologização da pecuária familiar na Serra do Sudeste.

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    Histórico; Local de execução e perfil da população; Identificação dos elementos e interações; Modelo sistêmico / Sistemas de produção.bitstream/item/63681/1/DT98.pd

    Avaliação de populações de azevém quanto à produção de forragem.

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    bitstream/item/65156/1/CNPGL-BOL.-PESQ.-15-04.pd

    Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR)

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    <p>Abstract</p> <p>Background</p> <p>Human bocavirus (HBoV) is a recently discovered parvovirus associated with mild to severe lower respiratory tract infections in children, the aim of the work was determination of human bocavirus in nasopharyngeal aspirate (NPA) of infants by qualitative PCR and determination of acute human bocavirus infection by estimation of immunoglobulin M (IgM) antibodies in serum by enzyme linked immunosorbent assay.</p> <p>Results</p> <p>Twenty two (22%) out of the 100 NPA specimens of the patients with respiratory manifestations were positive for HBoV by qualitative PCR, while ELISA revealed positive HBoV IgM antibodies in 18 (18%) patients who were also positive by PCR. Non of the controls were positive by both techniques. The correlation study between ELISA and PCR revealed high significant association, (p < 0.001, X<sup>2 </sup>= 36 and agreement = 96%). Also PCR detected 4 (18.1%) NPA samples as HBoV positive cases among the patients that were not identified by ELISA. This could be due to high sensitivity and efficacy of PCR. ELISA being less sensitive than RT-PCR, sensitivity was (81.8% vs 100%), the efficacy was 97.7% in ELISA versus 99.7% for RT-PCR.</p> <p>Conclusion</p> <p>HBoV infections could be diagnosed in NPA of children by conventional PCR as a rapid and sensitive technique. While ELISA was a reliable serologic analysis for diagnosis of acute HBoV infection by estimation IgM antibodies in serum.</p
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