The efficiency of Leguminoceae Production Grains Plus Maize as A Feeding on Growth Phase of Local Swine (Potamochoerus porcus) in Timor-Leste

In Timor Leste, swine Farmers did not yet use legume production grains to maintain feed swine performance and respond to market demand. The research aims to evaluate the Efficiency of Leguminoceae Production grains Plus Maize in feeding on the Growth Phase of Local Swine in Timor Leste. This research used Latin Square Design with four rows and four columns employed. The swine feeding has 4 treatments such as R1 (corn 50% + mungbean 15% + soybean 20% + common bean 15%), R2 (corn 40% + mungbean 20% + soybean 15% + common bean 25%), R3 (corn 40% + mungbean 20% + soybean 20% + common bean 20%), R4 (corn 50% + mungbean 10% + soybean 15% + common bean 25%). This research showed that Swine that consumed R2 rates were very good compared to other feedings for Swine. Look at the performance of the Swine with an increase in body weight of 31.16kg and body length of 72.75cm in the grower phase. All data were analyzed by ANOVA Latin Square Design, and Least Significance Difference (LSD) was conducted when the means were significantly different (p<0.05)

Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy

Conjugated linoleic acid (CLA), a fatty acid found in ruminant food products, has been associated with anticarcinogenic activity. However, its effect on cancer metabolism is unclear. In this paper we evaluated the effects of cis-9, trans-11 CLA on the metabolic profile of MCF-7 and MDA-MB-231 breast cancer cells using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. The NMR spectra showed that phosphocholine level, a cancer malignance biomarker, was reduced in both cells treated with CLA, but the reduction was more pronounced in MCF-7 cells. The NMR spectra also showed that CLA has opposite effect on MCF-7 and MDA-MB-231 acetone metabolism. Acetone signal has been observed in the spectra of MDA-MB-231 control cells, but not in the spectra of the cells treated with 50 and 100 µM CLA. Conversely, the acetone signal is very small or not observed in the NMR spectra of MCF-7 control cells and in cells treated with 50 µM of CLA, but is very strong in the spectra of the cells treated with 100 µM of CLA. Therefore, this CLA concentration is causing a ketosis in MCF-7 cells by inducing the use of fatty acids as an energy source or by reducing acetone catabolism. These results indicate that CLA interfere in the metabolism of both cells. However, the strongest effect has been observed on the metabolism of MCF-7 cells cultivated in the presence of 100 µM CLA. Therefore, CLA could be a potential anticarcinogenic drug, especially for cells with positive estrogen receptor, such as MCF-7

Lectins: Function, structure, biological properties andpotential applications

Lectins are a special class of proteins widely distributed in nature, which selectively recognize and reversibly bind to carbohydrates and glycoconjugates through their binding sites. These proteins, which can be detected through haemagglutination assays, interact with different carbohydrates present in cell surfaces. Lectins are generally classified according to their structure, specificity for carbohydrates and species location. Depending on their properties and distribution in tissues, lectins can play important physiological roles. The characteristic property of lectins to recognize other molecules in a distinct way makes it relevant in research involving purification, structural analysis, in vitro/in vivo applications of these macromolecules and biotechnological uses in different areas such as molecular and cell biology, immunology, pharmacology, medicine, clinical analysis, nanotechnology as well as in systems for drug release. Lectins can be used for analysis of structure and physiology of cells, tissues and pathogenic microorganisms. In agriculture, these proteins are used as insecticidal agents. Lectins have already been shown to exhibit different biological activities and effects, such as mitogenic and antiproliferative activities on cell lines of human cancer, inhibition of bacterial and fungal growth, action as promoting agents in cell aggregation, immunomodulatory activities and toxic effects. These proteins are promising as drugs for treatment and in diagnosis of human diseases; they are important tools in cytochemistry, histochemistry and immunohistochemistry and are also useful in forensic medicine. In summary, this review provides an overview of lectin research, with focus on physiological functions, structural performance, classification, potential biotechnological properties and applications.Authors acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for fellowships to PMGP, MTSC and LCBBC. In addition, we are grateful to Grant SFRH/BPD/ 37349/2007 from the Foundation for Science and Technology and POPH/FSE, awarded to AFS

Períodos de interferência das plantas daninhas na cultura do girassol em Rondônia Periods of weed interference in sunflower crop in Rondônia

Objetivou-se, neste trabalho, determinar os períodos de interferência das plantas daninhas na cultura do girassol e seus efeitos sobre o diâmetro do capítulo, teor de óleo, rendimento de óleo e produtividade. Adotou-se o delineamento experimental de blocos ao acaso com três repetições. Os tratamentos foram distribuídos em esquema fatorial 2 x 2 x 5, sendo os fatores: cultivares (Embrapa 122 e Hélio 358), modalidade de competição (na presença e na ausência de plantas daninhas) e períodos de convivência ou controle das plantas daninhas da emergência aos 15, 30, 45, 60 e 110 dias. As variáveis mensuradas foram diâmetro de capítulo, teor de óleo, rendimento de óleo e produtividade. As plantas daninhas interferem negativamente no diâmetro do capítulo, produtividade e rendimento de óleo, mas o convívio com as mesmas aumentou o teor de óleo das sementes, além de reduzir em 76,83 e 92,68% a produtividade dos cultivares Embrapa 122 e Hélio 358, respectivamente. Admitindo-se uma perda de 10% na produtividade, o período anterior à interferência (PAI), o período total de prevenção a interferência (PTPI) e o período crítico de prevenção e interferência (PCPI) para a cultivar Embrapa 122 foram de 16; 37 e 21 dias, respectivamente. Para o híbrido Hélio 358 o PAI, o PTPI e o PCPI foram de 24; 43 e 19 dias, respectivamente.<br>The objective of this study was to determine the periods of weed interference in sunflower cultivation, and its effects on the head diameter, oil content, oil yield and its productivity. The experimental design was in the randomized blocks with three replications. The treatments were arranged in a factorial 2 x 2 x 5, with the following factors: cultivar (Embrapa 122 and Hélio 358), modality of competition (in the presence and absence of weeds) and periods of coexistence and control of weed from the emergence until the 15, 30, 45, 60 and 110 days. The measured variables were head diameter, oil content, oil yield and productivity. Weeds interfere negatively in the head diameter, in the productivity, and in the yield of oil, but the coexistence with the weeds increases the oil content of seeds, as well as reduces in 76.83 and 92.68% the productivity of cultivars Embrapa 122 and Hélio 358, respectively. Assuming a 10% loss in productivity, the PAI, PTPI and the critical period of prevention and interference (PCPI) for the cultivar Embrapa 122 were 16, 37 and 21 days, respectively. For the hybrid Hélio 358 the period before interference (PAI), the PTPI and the PCPI were 24, 43 and 19 days, respectively