THE EFFECTS OF HOMOCYSTEINE ON PLASMA BIOCHEMICAL PARAMETERS AND AORTIC MATRIX METALLOPROTEINASES ACTIVITIES

Objective; Increased levels of physiological amino acid homocysteine (Hcy) in plasma is associated with the development of cardiovascular, neuronal, and liver diseases.[1] Damage of the vascular wall of aorta develops by exposure of vessel to the not yet degraded, toxically acting hcy, as occurs in the beginning of arteriosclerosis.[2]Methods; Our investigation was performed in two parts on 12 male wistar rats, 3-4 months old in both. First we investigated the relation between homocysteine and other plasma biochemical parameters which is related with cardiovascular events: Total cholesterol (CT), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), triglyceride (TG). We employed 3 groups: (C) control. (Met) rats received methionine in high doses (200mg/Kg/day), (Met-C) in order to potentiate the atherogenic effect cholesterol was administred (1500mg/Kg/day). Lipid parameters were measured, and structural damage to an aorta was analyzed by histology.In the second part, we used 3 groups: (C) control. (M) methionine (1g/Kg/day), (H) Hcy (20mg/day) to compare the effects of both methionine and homocysteine on aortic MMPs expression.Results & Conclusion: The results show that elevated plasma homocysteine increase cholesterol synthesis, exerts an angiotoxic action direct to aorta (loss of endothelium, degeneration partly with dissolution of media cells), and induce expression of MMP-2, while MMP-9 was not expressed

METHIONINE SUPPLEMENTATION INDUCES THYMUS VEGF-A EXPRESSION AND HEMATOLOGICAL CHANGES IN RATS

Objective: The present study evaluates the effects of methionine supplementation on the thymus histology and the peripheral blood in albino Wistar rats.Methods: The animals were divided into two groups. The experimental group (Met)(500mg/Kg /day, orally) and the control (C). Blood was collected for C reactive protein (CRP), total protein, Malondialdehyde (MDA) and blood smear tests, then thymuses were harvested for Histology and immune histochemistry.Results: Methionine intake caused an increase in CRP (P<0, 01), total protein (P<0,001) and MDA levels (P<0, 01) in addition to several hematological changes expressed by crenation of red blood cell membranes and irregular leucocyte nuclei. Histological investigation of experimental thymus gave dramatic involution of lobules and presented of apoptotic zones especially in the medulla. Immuno histochemical expression of VEGF- A were positive in septa and blood vessels at the corticomedullary junction. Conclusion: Methionine supplementation produces hematological changes, a thymic involution, and seemed to promote the angiogenesis process in the thymus of adult rats.Keywords: Methionine, Thymus, Blood cells, VEGF-A, Histopathology, Involution, Angiogenesis

HYPERHOMOCYSTEINEMIA LEAD TO TRANSMURAL INFLAMMATION OF COLON AND INCREASE SEVERITY OF DISEASE IN ACETIC ACID-INDUCED COLITIS IN RAT

Objective: The present study was designed to evaluate the effect of hyperhomocysteinemia (Hhcy) induced by feeding rats high methionine diet on the colon wall. Colonic damages caused by Hhcy were compared with those induced by acetic –acid induced colitis.Methods: Sprague-Dawley rats (200-250g) were divided into four groups: group C (control), group M (received 1 g/kg methionine p. o. during 15 d), group A (colitis was induced by transrectal administration of acetic acid 4% on 8th day) and group MA (received methionine and acetic acid). At the end of the study, plasma homocysteine, C-reactive protein (CRP) and leukocytes (WBC) count were evaluated, all rats were sacrificed and distal 8 cm of the colon was dissected. Colon was weighed for disease activity index (DAI) and injuries were assessed macroscopically and histologically.Results: High methionine diet induced significant (P<0.001) increase of homocysteine (hcy), CRP levels and WBC count compared to control. Acetic acid rats showed a significant decrease of WBC count. Mixed treatment caused a significant increase of hcy, CRP and a significant decrease of WBC count. Our results showed that Hhcy causes significant damages and immune cells infiltration in all layers of the colonic wall.Conclusion: The present investigation demonstrated that Hhcy increased the major inflammatory markers as CRP and leukocytes count and produced transmural colitis in rats. Effect of Hhcy is more toxic on the colonic wall than acetic acid indeed while acetic acid lesions are localized in mucosa and submucosa the lesions of hcy extend to the all layers (mucosa, submucosa and muscularis propria). Acetic acid induced colitis in hyperhomocysteinemic rats increased the severity of colitis

COMPARATIVE STUDY OF TWO HELIX ASPERSA EXTRACTS ON TUMOR CELL LINES (HUT-78 AND SEAX) PROLIFERATION AND MMP-9 SECRETION

Objective: Despite the progress in cancer research, current therapies are ineffective and cause many adverse effects. The discovery of new natural anti-tumor agents that can act on multiple mechanisms of growth and tumor invasion with minor side effects and which can be safe for patients. Therefore, we sought new natural products from an invertebrate organism belonging to the phylum of mollusks: a land snail, Helix aspersa. This study was aimed to evaluate the cytotoxic activity of two extracts prepared from Helix aspersa, on two cutaneous T cell lymphoma cell line (HUT-78 and SeAx). Their effect on MMP-9 expression was also tested.Methods: We prepared from the snail Helix aspersa: an aqueous (AE) and a hydroalcoholic extracts (HAE). We have evaluated the concentration of total proteins and total phenols in these extracts. The percentage of cell mortality was evaluated after incubating the cell lines with the two extracts at different concentrations, by using trypan blue exclusion method. Finally, the tumor cells expression of metalloproteinase MMP-9 was examined by zymography analysis.Results: We have found that 1 mg/ml of AE contains (4.53±0.48 mg) of total proteins and (2.44±0.11 mg) GAE g-1of phenols. 1 mg/ml of HAE contains (1.83±0.23 mg) of total proteins and (2.81±0.16 mg) GAE g-1 of phenols. On one hand, both extracts exerted a toxic effect on these tumor cells. Indeed, aqueous extract induced 44.09 % mortality in HUT-78 and 31.47 % mortality in SeAx tumor cell line with 50 µg/ml. While, the hydroalcoholic extract induced 29.90 % in HUT-78 and 25 % in SeAx. On the other hand, the result showed no changes of MMP-9 expression.Conclusion: Helix aspersa's extracts had a toxic effect on cutaneous T cell lymphoma (CTCL), but did not inhibit the production of the protease by these two cell lines in culture

Evaluation of the artherogenic potential of homocysteine and its cytotoxic effect on HUVECs

Background: In recent decades, several studies have shown that hyperhomocysteinemia is an independent risk factor for atherosclerosis. The objective of this work is, first, to assess the atherogenic potential of homocysteine in rats and and appraise its effect on endothelial cells: the main components of the blood vessel. Methods: In the in vivo study, hyperhomocysteinemia is induced in rats by a diet supplemented with L-methionine (1 g/kg/day). After 21 days of treatment, a blood sample is performed for biochemical study (total cholesterol, triglycerides, HDL, LDL cholesterol, ALT, AST, CRP, and total protein) and a removal of organs (aorta, liver, and heart) for the assay MDA and histological study. For the in vitro study, HUVECs were cultured for 24 h then exposed to various concentrations of homocysteine (2 mM, 4 mM, 8 mM). After 24 hours, we confirm the cellular phenotype and its number. Results: The angiotoxic dose of methionine induces a significant increase for all biochemical parameters (except HDL-C, which is diminished). In addition, an alteration of the histological structure of the aorta (appearance of foam cells) of the liver (steatosis) and of the heart. The in vitro study revealed a senescent phenotype of HUVECs and an inhibition of cell proliferation. Conclusion: Our results suggest that homocysteine is involved in the genesis of atherosclerosis by affecting directly the endothelial cells

Purification, proprietes physico-chimiques et etude de la secretion gastrique du facteur intrinseque en cas de mucoviscidose

SIGLECNRS T 60090 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

HYPERHOMOCYSTEINEMIA LEAD TO TRANSMURAL INFLAMMATION OF COLON AND INCREASE SEVERITY OF DISEASE IN ACETIC ACID-INDUCED COLITIS IN RAT

Objective: The present study was designed to evaluate the effect of hyperhomocysteinemia (Hhcy) induced by feeding rats high methionine diet on the colon wall. Colonic damages caused by Hhcy were compared with those induced by acetic –acid induced colitis.Methods: Sprague-Dawley rats (200-250g) were divided into four groups: group C (control), group M (received 1 g/kg methionine p. o. during 15 d), group A (colitis was induced by transrectal administration of acetic acid 4% on 8th day) and group MA (received methionine and acetic acid). At the end of the study, plasma homocysteine, C-reactive protein (CRP) and leukocytes (WBC) count were evaluated, all rats were sacrificed and distal 8 cm of the colon was dissected. Colon was weighed for disease activity index (DAI) and injuries were assessed macroscopically and histologically.Results: High methionine diet induced significant (P<0.001) increase of homocysteine (hcy), CRP levels and WBC count compared to control. Acetic acid rats showed a significant decrease of WBC count. Mixed treatment caused a significant increase of hcy, CRP and a significant decrease of WBC count. Our results showed that Hhcy causes significant damages and immune cells infiltration in all layers of the colonic wall.Conclusion: The present investigation demonstrated that Hhcy increased the major inflammatory markers as CRP and leukocytes count and produced transmural colitis in rats. Effect of Hhcy is more toxic on the colonic wall than acetic acid indeed while acetic acid lesions are localized in mucosa and submucosa the lesions of hcy extend to the all layers (mucosa, submucosa and muscularis propria). Acetic acid induced colitis in hyperhomocysteinemic rats increased the severity of colitis

Constitutive AKT activation in follicular lymphoma.

International audienceBackground:The phosphoinositide 3- kinase (PI3K) pathway is involved in the growth of various human cancers,including lymphoid malignancies. However its role in the pathogenesis of follicular lymphoma (FL) has not beenyet described.Methods:To clarify this point, biopsy tissue samples from 38 human FL cases were investigated for PIK3CA somaticmutations in exon 9 and 20 using direct sequencing. The same samples were analyzed using western blotting andimmunohistochemistry to detect expression of AKT, phosphorylated AKT (pAKT), and PTEN proteins. Two cases ofbenign lymphadenitis were used as controls.Results:AKT expression was present in all FL and lymphadenitis cases. 14/38 (37%) FL and 2/2 lymphadenitis casesexpressed pAKT. 9/38 (24%) FL samples showed high level of pAKT, whereas 5/38 (13%) FL cases and 2/2 benignlymphadenitis samples expressed low level of pAKT. PTEN expression was observed in 30/38 (79%) FL and 2/2benign lymphadenitis cases, whereas 8/38 (21%) FL cases showed loss of PTEN expression. 3 cases with positivepAKT did not express PTEN. PIK3CA mutations were not detected in any sample.Conclusions:These data suggest that the PI3K/AKT signaling pathway could be activated in a subset of FL cases,due to either AKT phosphorylation or PTEN downregulation, in the absence of PIK3CA mutations

Abstracts of 1st International Conference on Computational & Applied Physics

This book contains the abstracts of the papers presented at the International Conference on Computational & Applied Physics (ICCAP’2021) Organized by the Surfaces, Interfaces and Thin Films Laboratory (LASICOM), Department of Physics, Faculty of Science, University Saad Dahleb Blida 1, Algeria, held on 26–28 September 2021. The Conference had a variety of Plenary Lectures, Oral sessions, and E-Poster Presentations. Conference Title: 1st International Conference on Computational & Applied PhysicsConference Acronym: ICCAP’2021Conference Date: 26–28 September 2021Conference Location: Online (Virtual Conference)Conference Organizer: Surfaces, Interfaces, and Thin Films Laboratory (LASICOM), Department of Physics, Faculty of Science, University Saad Dahleb Blida 1, Algeria