A concise review on the use of mesenchymal stem cells in cell sheet-based tissue engineering with special emphasis on bone tissue regeneration

The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering. This review will focus on the use of MSC sheets in osteogenic tissue engineering. Potential application techniques with or without scaffolds and/or grafts will be discussed. Finally, the importance of osteogenic induction of these MSC sheets in orthopaedic applications will be demonstrated. © 2017 A. Cagdas Yorukoglu et al

Erratum: Investigation of microRNA expression changes in HepG2 cell line in presence of URG4/URGCP and in absence of URG4/URGCP suppressed by RNA interference (Molecular Biology Reports (2012) 39 (11119-11124) DOI: 10.1007/s11033-012-2019-8)

[No abstract available]2010BSP023The following information was missed out in the original published article: Acknowledgments This work was supported by Pamukk-ale University Research Projects (Grant No: 2010BSP023). -

HBV X protein: Elucidating a role in oncogenesis

Chronic HBV infection is associated with the development of hepatocellular carcinoma (HCC). HBV contributes to tumorigenesis by encoding hepatitis B x antigen (HBxAg), which is a trans-regulatory protein that appears to contribute to HCC by altering patterns of host gene expression. In this review, recent data is presented that outlines some of the putative mechanisms whereby HBxA9 contributes to HCC. With the development of animal models of HBxAg-mediated HCC, the relevance and temporal order of putative steps in this process can now be dissected to elucidate what is rate limiting and when. This will have a profound impact on the design of novel and specific therapeutics for HCC. © 2008 Future Medicine Ltd

Regulation of URG4/URGCP and PPAR? gene expressions after retinoic acid treatment in neuroblastoma cells

Neuroblastoma (NB), originating from neural crest cells, is the most common extracranial tumor of childhood. Retinoic acid (RA) which is the biological active form of vitamin A regulates differentiation of NB cells, and RA derivatives have been used for NB treatment. PPAR? (peroxisome proliferator-activated receptor) plays an important role in the oxidation of fatty acids, carcinogenesis, and differentiation. URG4/URGCP gene is a proto-oncogene and that overexpression of URG4/URGCP is associated with metastasis and tumor recurrence in osteosarcoma. It has been known that URG4/URGCP gene is an overexpressed gene in hepatocellular carcinoma and gastric cancers. This study aims to detect gene expression patterns of PPAR? and URG4/URGCP genes in SH-SY5Y NB cell line after RA treatment. Expressions levels of PPAR? and URG4/URGCP genes were analyzed after RA treatment for reducing differentiation in SH-SY5Y NB cell line. To induce differentiation, the cells were treated with 10 µM RA in the dark for 3-10 days. Gene expression of URG4/URGCP and PPAR? genes were presented as the yield of polymerase chain reaction (PCR) products from target genes compared with the yield of PCR products from the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. SH-SY5Y cells possess small processes in an undifferentiated state, and after treatment with RA, the cells developed long neurites, resembling a neuronal phenotype. PPAR? gene expression increased in RA-treated groups; URG4/URGCP gene expression decreased in SH-SY5Y cells after RA treatment compared with that in the control cells. NB cell differentiation might associate with PPAR? and URG4/URGCP gene expression profile after RA treatment. © 2013 International Society of Oncology and BioMarkers (ISOBM)

Enalapril-induced acute retal failure and jugular venous thrombosis associated with a homozygous methylene tetrahydrofolate reductase A1298C polymorphisms in a newborn with central venous line: Case report

A full term newborn having no underlying renal disease developed acute renal failure (ARF) after starting of oral enalapril (0.01mg/kg/day) given for the treatment of congestive heart failure (CHF). After peritoneal dialyses, she recovered from ARF. The patient treated due to yen tilatory associated pneumonia, sepsis and candidemia, developed left juguler venous thrombosis as sociated with central venous line by day 51 of life. Enoxaparine dose started 1.5 mg/kg/twice daily subcutaneously, was increased until reach recommended therapeutic target range of antifactor Xa. While she was treated with enoxaparine at a dose of 1.9 mg/kg/twice daily, thrombus propa gation was detected at her right juguler vein. Homozygote methylene tetrahydrofolatereductase (MTHFR) gene A1298C polymorphism was detected at the ethiology. Plasma homocysteine level and other prothrombotic components were normal. We suggest that, MTHFR gene A1298C poly morphism might be investigated in infants with ARF, venous thrombosis in spite of normal homo cysteine levels in the presence of prothrombotic risk factors such as CHF, sepsis, and catheter placement.. Copyright © 2013 by Türkiye Klinikleri

Detection of Y chromosomal material in patients with a 45,X karyotype by PCR method

A 45,X karyotype is one of the common chromosomal abnormalities characterized by short stature, lack of development of secondary sexual characteristics, webbed neck and cubitus valgus. This phenotype was described by Turner in 1938 and was called Turner syndrome (TS). About 40-60% of the patients with TS phenotype have a 45,X karyotype, the rest either have a structurally abnormal X or Y chromosome or mosaicism with a second cell line. Determination of Y chromosome derivatives in patients with a 45,X karyotype is important for the management of these patients due to increased risk of gonadoblastoma. Low level mosaicisim of Y chromosome may be missed by cytogenetic methods. The aim of our study is to analyze cryptic Y chromosome derivatives using Y specific sequences in 40 Turkish patients with a pure 45,X karyotype. Fourteen different Y specific sequences along the Y chromosome were selected for the detection of cryptic Y chromosome material by PCR analysis. The present study demonstrated that 2 patients with a 45,X karyotype (5%) have Y specific sequences except sex releated region Y (SRY). One of them had displayed enhanced virilisation whereas other showed no virilisation. In conclusion, it has been found by PCR analysis that 5% of patients with a 45,X karyotype have Y chromosome sequences in the absence of any marker chromosome by cytogenetic analysis. The data also suggest that the patients with a 45,X karyotype should be analyzed for the presence of Y chromosome derivatives by sensitive mehtods, such as PCR, in order to calculate the future risk of developing gonadoblastoma. © 2007 Tohoku University Medical Press