Understorey plant community and light availability in conifer plantations and natural hardwood forests in Taiwan

Questions: What are the effects of replacing mixed species natural forests with Cryptomeria japonica plantations on understorey plant functional and species diversity? What is the role of the understorey light environment in determining understorey diversity and community in the two types of forest? Location: Subtropical northeast Taiwan. Methods: We examined light environments using hemispherical photography, and diversity and composition of understorey plants of a 35‐yr C. japonica plantation and an adjacent natural hardwood forest. Results: Understorey plant species richness was similar in the two forests, but the communities were different; only 18 of the 91 recorded understorey plant species occurred in both forests. Relative abundance of plants among different functional groups differed between the two forests. Relative numbers of shade‐tolerant and shade‐intolerant seedling individuals were also different between the two forest types with only one shade‐intolerant seedling in the plantation compared to 23 seedlings belonging to two species in the natural forest. In the natural forest 11 species of tree seedling were found, while in the plantation only five were found, and the seedling density was only one third of that in the natural forest. Across plots in both forests, understorey plant richness and diversity were negatively correlated with direct sunlight but not indirect sunlight, possibly because direct light plays a more important role in understorey plant growth. Conclusions: We report lower species and functional diversity and higher light availability in a natural hardwood forest than an adjacent 30‐yr C. japonica plantation, possibly due to the increased dominance of shade‐intolerant species associated with higher light availability. To maintain plant diversity, management efforts must be made to prevent localized losses of shade‐adapted understorey plants

Integrin activation - the importance of a positive feedback

Integrins mediate cell adhesion and are essential receptors for the development and functioning of multicellular organisms. Integrin activation is known to require both ligand and talin binding and to correlate with cluster formation but the activation mechanism and precise roles of these processes are not yet resolved. Here mathematical modeling, with known experimental parameters, is used to show that the binding of a stabilizing factor, such as talin, is alone insufficient to enable ligand-dependent integrin activation for all observed conditions; an additional positive feedback is required.Comment: in press in Bulletin of Mathematical Biolog

Seller’s optimal credit period and replenishment time in a supply chain with up-stream and down-stream trade credits

[[abstract]]In practice, a supplier often offers its retailers a permissible delay period M to settle their unpaid accounts. Likewise, a retailer in turn offers another trade credit period N to its customers. The benefits of trade credit are not only to attract new buyers who consider it a type of price reduction, but also to provide a competitive strategy other than introduce permanent price reductions. On the other hand, the policy of granting credit terms adds an additional cost to the seller as well as an additional dimension of default risk. In this paper, we first incorporate the fact that trade credit has a positive impact on demand but negative impacts on costs and default risks to establish an economic order quantity model for the seller in a supply chain with up-stream and down-stream trade credits. Then we derive the necessary and sufficient conditions to obtain the optimal replenishment time and credit period for the seller. Finally, we use some numerical examples to illustrate the theoretical results.[[incitationindex]]SCI[[booktype]]電子

Etching characteristics and mechanism of Ba0.7Sr0.3TiO3 thin films in an inductively coupled plasma

The inductively-coupled-plasma (ICP) etching behavior of Ba0.7ST0.3TiO3 (BST) thin films has been characterized with Cl-2/CF4, Cl-2/SF6 and Cl-2/Ar gas mixtures. CF4 and SF6 were found to impede the etch process, presumably due to competition between plasma deposition and etching. A chemically assisted etch of BST was obtained under various Cl-2/Ar gas mixtures. The etch profile along with etch anisotropy was observed as a function of etching parameters by scanning electron microscopy. The surface morphologies after etching were analyzed by atomic force microscopy. A smooth surface (roughness similar to 1.8 nm) with no residue was observed under 30%Cl-2 in Ar/Cl-2, ICP power of 1 kW, substrate bias of 500 V, and 10 mTorr. To clarify the etching mechanism, the surface reaction of the BST thin films was investigated by X-ray photoelectron spectroscopy. It was found that Ba was mainly removed by chemically assisted physical etching (possible products such as BaClx). Physical bombardment is more effective than Cl chemical reaction for removing Sr, while Ti can almost be removed by chemical reaction (such as TiClx). The etching results described correlate well with the thermochemical calculations

Etching of platinum thin films in an inductively coupled plasma

Experimental studies of the etching of platinum thin films have been performed with a photoresist mask in an inductively coupled plasma. The physical bombardment by incident Ar ions dominates the platinum etch rate. In order to minimize the formation of sidewall deposition, the effects of the addition of various halogen gases to Ar plasma were evaluated. For the blanket platinum samples etched in Ar/CF4 plasmas, the existence of Pt-F compounds was found by using secondary ion mass spectrometry, By adding CFS in Ar/Cl-2 gas plasmas, an increase of etch rate for platinum films was observed. This suggests that the addition of CF4 to the Ar/Cl-2 gas mixture could enhance the reaction between platinum and fluorine on the platinum surface by providing more fluorine radicals and ions. The respective etch contribution provided by the three components (Ar, CF4 and Cl-2) has been investigated. A fence-free platinum electrode can be obtained under an optimum Ar/CF4/Cl-2 gas mixture ratio, resulting in an etch rate of 48 nm/min. (C) 2001 Elsevier Science B.V. All rights reserved

Quantification on the source/receptor relationship of primary pollutants and secondary aerosols by a Gaussian plume trajectory model: Part III-Asian dust-storm periods

Characteristics of pollutants at heights in the top of the Planetary Boundary Layer (PBL) are collected and used in a local-scale model. A subsidence mechanism is developed to quantify the concentration fraction from the top PBL to simulate PM concentration during Asian dust-storm (ADS) periods. The results show that using the data measured at a mountain station, which is very vulnerable to ADS, as the top boundary conditions for the air quality model can capture all the PM2.5 episodes due to local sources and ADS events, at a low-altitude urban station. The correlation coefficient (r(2)) of daily PM2.5-10 concentration has increased from 0.17 to 0.62 by incorporating the subsidence mechanism, and that of PM2.5 increases as well. The model results of nitrate, sulfate and ammonium aerosol in fine radii can be compared with observations. According to our analysis, five out of eight PM2.5 or PM10 episode days occurred on ADS days in the past 4 years (1999-2002). During ADS episodes in 2000, 12% of PM2.5 and 53% of PM2.5-10 were from ADS dust. In addition, two dry deposition algorithms are evaluated; the algorithm of Seinfeld and Pandis (Atmospheric Chemistry and Physics from Air Pollution to Climate Change, Wiley, New York, 1998, 1057pp.) is suggested in this case study. (C) 2003 Elsevier Ltd. All rights reserved

Discovery of Enterovirus A71-like nonstructural genomes in recent circulating viruses of the Enterovirus A species

Enterovirus A71 (EV-A71) is an important nonpolio enterovirus that causes severe neurological complications. In 1998, Taiwan experienced an EV-A71 outbreak that caused 78 deaths. Since then, periodic epidemics of EV-A71 associated with newly emerging strains have occurred. Several of these strains are known to be recombinant; however, how these strains arose within such a short period of time remains unknown. Here, we sequenced 64 full-length genomes from clinical isolates collected from 2005 to 2016 and incorporated all 91 Taiwanese genomes downloaded from the Virus Pathogen Resource to extensively analyze EV-A71 recombination in Taiwan. We found that the B3 subgenotype was a potential recombinant parent of the EV-A71 C2-like and C4 strains by intratypic recombination. Such B3-similar regions were also found in many cocirculating coxsackieviruses belonging to Enterovirus A species (EV-A) through a series of intertypic recombinations. Therefore, locally enriched outbreaks of cocirculating viruses from different genotypes/serotypes may facilitate recombination. Most recombination breakpoints we found had nonrandom distributions and were located within the region spanning from the boundary of P1 (structural gene) and P2 (nonstructural) to the cis-Acting replication element at P2, indicating that specific genome reassembly of structural and nonstructural genes may be subject to natural selection. Through intensive recombination, 11 EV-A71-like signatures (including one in 3A, two in 3C, and eight in 3D) were found to be present in a variety of recently cocirculating EV-A viruses worldwide, suggesting that these viruses may be targets for wide-spectrum antiviral development.BN/Nynke Dekker La

Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses

Viral RNA-dependent RNA polymerases (RdRps) are a target for broad-spectrum antiviral therapeutic agents. Recently, we demonstrated that incorporation of the T-1106 triphosphate, a pyrazine-carboxamide ribonucleotide, into nascent RNA increases pausing and backtracking by the poliovirus RdRp. Here, by monitoring enterovirus A-71 RdRp dynamics during RNA synthesis using magnetic tweezers, we identify the “backtracked” state as an intermediate used by the RdRp for copy-back RNA synthesis and homologous recombination. Cell-based assays and RNA sequencing (RNA-seq) experiments further demonstrate that the pyrazine-carboxamide ribonucleotide stimulates these processes during infection. These results suggest that pyrazine-carboxamide ribonucleotides do not induce lethal mutagenesis or chain termination but function by promoting template switching and formation of defective viral genomes. We conclude that RdRp-catalyzed intra- and intermolecular template switching can be induced by pyrazine-carboxamide ribonucleotides, defining an additional mechanistic class of antiviral ribonucleotides with potential for broad-spectrum activity.Accepted Author ManuscriptBN/Cees Dekker LabBN/Nynke Dekker La