Privacy in transformational government (t-government): an investigation of citizens' privacy preferences and perceptions

No Abstract

Removal of Methylene Blue from Aqueous Solutions using Chemical Activated Carbon Prepared from Jackfruit (Artocarpus heterophyllus) Peel Waste

Dye wastewater generated is rated as the most polluting wastewater among all the industrial sectors. Adsorption using activated carbon (AC) has been proven to be effective to treat dye wastewater. In this study, jackfruit (Artocarpus heterophyllus) peel waste has been utilized for activated carbon (AC) preparation using chemical activation. This research attempts to study the factors affecting its adsorption performance. Series of experiments conducted consisted of the experiments studying the effect of initial dye concentration and also effect of adsorbent dosage. In the study, CAC showed adsorption capacity of 10.43 mg/g

Physiological responses of callus from gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation

In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9 of exposure to gamma irradiation. Reduction of soluble protein content was observed in all the treatments as the increase of incubation period

Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’

Studies of Fortunella polyandra ‘Nagami’ and ‘Meiwa’ were carried out to observe their responses in tissue culture systems. Explant sources investigated included roots, stems, leaves and cotyledons which were cultured on Murashige and Skoog (MS) medium supplemented with different combinations and concentrations of various hormones. The hormones used were naphthalene acetic acid (NAA) and benzylaminopurine (BAP) at pH 5.8 with a temperature of 23-26°C and a photoperiod of 16 hours light and 8 hours dark. The pH of the media was also altered to include 4.8, 5.8, 6.8 and 7.8. The various explants were subjected to light and dark treatments in order to study the morphogenesis of this species. From the results, it was observed that stem and leaf explants were more responsive than other explants. The best media for regeneration and callus formation was MS supplemented with 0.5 mg/L NAA and 0.5 mg/L BAP at pH 5.8 for ‘Nagami’ and pH 7.8 for ‘Meiwa’. Regeneration was achieved via direct organogenesis and also via callus formation. The highest percentage of shoot formation (40%) was obtained from stem explants (‘Meiwa’) cultured on MS supplemented with 0.5 mg/L NAA and 0.5 mg/L BAP at pH 7.8

Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson

Comparative anatomical studies were carried out on in vivo plants of Triphasia trifolia (Burm. f.) P. Wilson and in vitro plantlets of the same age. To get the in vitro plantlets, explants were cultured on MS (Murashige and Skoog) media supplemented with different concentrations and combinations of hormones. The explant sources of Triphasia trifolia (Burm. f.) P. Wilson were obtained from cotyledons, leaves, stems, roots and shoot tips that were placed under conditions of 16 hours light and 8 hours dark. The optimum media for regeneration was MS supplemented with 1.0 mg/L BAP and 1.0 mg/L NAA. Cotyledon explants were found to be the most responsive. Regeneration of complete plantlets was achieved from cotyledon explants after about 4 months in culture. Sectioning was done to study the characteristics of the respective vascular bundles, shape of cells, palisade cell layers, presence of oil glands, druse and cuticle layers. Vascular bundles of in vivo leaves were extremely well developed compared to those in in vitro leaves. The vascular bundle of the in vivo leaf showed well developed xylem. However, the xylem and phloem cells of the in vitro leaf were very poorly developed which is one of the features of in vitro plants. Scanning electron microscope (SEM) studies were also carried out on the in vivo and in vitro plantlets to observe differences on the leaf surface

Privacy in transformational government (T-Government): Investigation of citizen’s privacy preferences and perceptions

No Abstract

Establishment of somatic embryogenesis from Gerbera jamesonii Bolus EX. Hook F. through suspension culture

Cell suspension cultures were established from embryogenic callus induced from leaf explants of Gerbera jamesonii Bolus ex. Hook f. Embryogenic callus was induced when leaf explants were cultured on MS medium containing 1.0 to 2.0 mg/L 2,4-D. Cream friable callus was formed within two weeks. Proliferated callus was transferred to MS liquid medium containing 2,4-D with a small concentration of NAA and subcultured at 2 weeks interval. Induction of somatic embryos (globular, heart and torpedo) was observed after 2 weeks of culture. Somatic embryos developed in MS suspension medium containing 1.0 to 2.0 mg/L 2,4-D with 0.1 or 1.0 mg/L NAA and globular embryos were further differentiated into the cotyledonary phase embryos. The addition of amino acids (L-glutamine or L-proline, 5.0 mg/L, respectively) to the culture media, in the range of concentrations tested, yielded higher enhancement of the embryo growth and development. Transfer of individual embryos onto a fresh basal medium with no plant growth regulators was able to achieve complete maturation. Relatively, only a few number of embryos developed shoots and roots when transferred to MS medium supplemented with 2.0 mg/L BAP and 0.5 mg/L NAA in addition to 3 (w/v) sucrose and 0.8 (w/v) agar containing medium. About 11 of somatic embryos were converted to true-to-type fertile plants

Growth optimization and organogenesis of Gerbera jamesonii Bolus ex. Hook f. in vitro

Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2,4-D), Indole-3-acetic acid (IAA), Indole-3-Butyric acid (IBA), N6-2-Isopentenyl adenine (2iP), Kinetin and Zeatin were used to initiate cultures. These plant growth regulators were added to Murashige and Skoog medium in different combinations and concentrations. Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 BAP and 0.5 mg L-1 NAA. Effectiveness of shoot regeneration medium, type of growth regulator used and duration of induction period were investigated. Leaf explants cultured on MS medium supplemented with 1.0 mg L-1 BAP and 2.0 mg L-1 2, 4-D showed the best results for callus induction. Root explants were found to be non-regenerative in all experiments conducted. Petiole segment was identified as the best explant for regeneration of this species. Regenerated plants were rooted on Murashige and Skoog basal medium. Plantlets were then transferred to field with 75% survival rate. © 2008 Asian Network for Scientific Information

Induction of somatic embryogenesis and plant regeneration in Begonia x hiemalis Fotsch. in vitro

Direct somatic embryogenesis induction of Begonia x hiemalis Fotsch. (Elatior Begonia) was initiated from two different explants i.e., leaves and petioles. Both explants were cultured on MS medium supplemented with different concentrations of Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic acid (2,4-D). The results showed that combinations of 0.5-1.0 mg L-1 BAP and 0.1 mg L-1 2,4-D produced direct somatic embryogenesis from leaf and petiole explants. Different concentrations of casein hydrolysate were also tested to optimize somatic embryo induction. The results showed that 100 mg L-1 casein hydrolysate could produce 53.08 nodular callus and 24.16 green embryogenic callus, whereas 500 mg L-1 casein hydrolysate produced 30.83 nodular callus and 23.75 green embryogenic callus. The embryogenic callus were then transferred to MS medium supplemented with 0.5 mg L-1 Gibberelic Acid (GA3) with 0.2 g L-1 activated charcoal for further embryogenesis development and further regeneration. © 2008 Asian Network for Scientific Information

Artificial seed production from encapsulated micro shoots of Saintpaulia ionantha Wendl. (African Violet)

Artificial seeds were produced from encapsulated micro shoots of Saintpaulia ionantha Wendl. (African violet). The production of artificial seeds of this species gave ideal beads based on firmness, texture, size and shape of beads. The percentage of germination from encapsulated micro shoots influenced by the concentrations of sodium alginate and calcium chloride (CaCl2.2H2O) used. It was found that among the concentrations tested, 3 sodium alginate and the exposure to 1.00 mM CaCl2.2H2O solution for 30 min had produced optimal beads with firm, clear, round and uniform size and suitable for handling. It was also observed that micro shoots obtained from optimization of encapsulation matrix showed the highest percentage of germination (84). Encapsulated micro shoots exposed for 30 min in 100 mM CaCl2.2H2O solution gave the optimal time of hardening process. The findings suggested that the encapsulation method for micro shoots could be used as an alternative to artificial seed derived from somatic embryos of Saintpaulia ionantha. © 2008 Asian Network for Scientific Information