Interplay between network structure and self-organized criticality

We investigate, by numerical simulations, how the avalanche dynamics of the Bak-Tang-Wiesenfeld (BTW) sandpile model can induce emergence of scale-free (SF) networks and how this emerging structure affects dynamics of the system. We also discuss how the observed phenomenon can be used to explain evolution of scientific collaboration.Comment: 4 pages, 4 figure

Universal scaling of distances in complex networks

Universal scaling of distances between vertices of Erdos-Renyi random graphs, scale-free Barabasi-Albert models, science collaboration networks, biological networks, Internet Autonomous Systems and public transport networks are observed. A mean distance between two nodes of degrees k_i and k_j equals to =A-B log(k_i k_j). The scaling is valid over several decades. A simple theory for the appearance of this scaling is presented. Parameters A and B depend on the mean value of a node degree _nn calculated for the nearest neighbors and on network clustering coefficients.Comment: 4 pages, 3 figures, 1 tabl

Thermodynamic forces, flows, and Onsager coefficients in complex networks

We present Onsager formalism applied to random networks with arbitrary degree distribution. Using the well-known methods of non-equilibrium thermodynamics we identify thermodynamic forces and their conjugated flows induced in networks as a result of single node degree perturbation. The forces and the flows can be understood as a response of the system to events, such as random removal of nodes or intentional attacks on them. Finally, we show that cross effects (such as thermodiffusion, or thermoelectric phenomena), in which one force may not only give rise to its own corresponding flow, but to many other flows, can be observed also in complex networks.Comment: 4 pages, 2 figure

Sustaining Electron Transfer Pathways Extends Biohybrid Photoelectrode Stability to Years

The exploitation of natural photosynthetic enzymes in semi-artificial devices constitutes an attractive and potentially sustainable route for the conversion of solar energy into electricity and solar fuels. However, the stability of photosynthetic proteins after incorporation in a biohybrid architecture typically limits the operational lifetime of biophotoelectrodes to a few hours. Here, we demonstrate ways to greatly enhance the stability of a mesoporous electrode coated with the RC-LH1 photoprotein from Rhodobacter sphaeroides. By preserving electron transfer pathways, we extended operation under continuous high-light to 33 days, and operation after storage to over two years. Coupled with large photocurrents that reached peak values of 4.6 mA cm−2, the optimized biophotoelectrode produced a cumulative output of 86 C cm−2, the largest reported performance to date. Our results demonstrate that the factor limiting stability is the architecture surrounding the photoprotein, and that biohybrid sensors and photovoltaic devices with operational lifetimes of years are feasible

Use of a Novel Imaging Technology for Remote Autism Diagnosis: A Reflection on Experience of Stakeholders

AbstractTimely diagnosis and early interventions are critical to improving the long term functioning of a child with ASD. However, a major challenge facing parents is difficulty in obtaining on-time access to appropriate diagnostic services. To address this need, an imaging technology, NODA® (Naturalistic Observation Diagnostic Assessment), has been successfully developed and field-tested. NODA® includes 1) NODA SmartCapture; a smart-phone based recording system for parents to capture and share in-home video evidence of their child behavior and 2) NODA Connect; a HIPPA compliant web-platform for diagnosticians to conduct remote autism diagnostic assessments based on in-home video evidence of behavior, developmental history and their clinical judgment. In the field study, parents captured and shared videos evidence from their homes via NODA SmartCapture and diagnosticians conducted remote diagnostic assessment via NODA Connect. Results show that parents were able to successfully collect video evidence of behavior as per given prescription and diagnosticians were able to complete remote diagnostic assessments. This paper is a reflection on the first hand experience of key stakeholders (parents and diagnosticians) using NODA® in the field

Average path length in random networks

Analytic solution for the average path length in a large class of random graphs is found. We apply the approach to classical random graphs of Erd\"{o}s and R\'{e}nyi (ER) and to scale-free networks of Barab\'{a}si and Albert (BA). In both cases our results confirm previous observations: small world behavior in classical random graphs $l_{ER} \sim \ln N$ and ultra small world effect characterizing scale-free BA networks $l_{BA} \sim \ln N/\ln\ln N$. In the case of scale-free random graphs with power law degree distributions we observed the saturation of the average path length in the limit of $N\to\infty$ for systems with the scaling exponent $2< \alpha <3$ and the small-world behaviour for systems with $\alpha>3$.Comment: 4 pages, 2 figures, changed conten

Intravital FRAP imaging using an E-cadherin-GFP mouse reveals disease- and drug-dependent dynamic regulation of cell-cell junctions in live tissue

E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments

Photoreversible interconversion of a phytochrome photosensory module in the crystalline state.

A major barrier to defining the structural intermediates that arise during the reversible photointerconversion of phytochromes between their biologically inactive and active states has been the lack of crystals that faithfully undergo this transition within the crystal lattice. Here, we describe a crystalline form of the cyclic GMP phosphodiesterases/adenylyl cyclase/FhlA (GAF) domain from the cyanobacteriochrome PixJ in Thermosynechococcus elongatus assembled with phycocyanobilin that permits reversible photoconversion between the blue light-absorbing Pb and green light-absorbing Pg states, as well as thermal reversion of Pg back to Pb. The X-ray crystallographic structure of Pb matches previous models, including autocatalytic conversion of phycocyanobilin to phycoviolobilin upon binding and its tandem thioether linkage to the GAF domain. Cryocrystallography at 150 K, which compared diffraction data from a single crystal as Pb or after irradiation with blue light, detected photoconversion product(s) based on Fobs - Fobs difference maps that were consistent with rotation of the bonds connecting pyrrole rings C and D. Further spectroscopic analyses showed that phycoviolobilin is susceptible to X-ray radiation damage, especially as Pg, during single-crystal X-ray diffraction analyses, which could complicate fine mapping of the various intermediate states. Fortunately, we found that PixJ crystals are amenable to serial femtosecond crystallography (SFX) analyses using X-ray free-electron lasers (XFELs). As proof of principle, we solved by room temperature SFX the GAF domain structure of Pb to 1.55-Å resolution, which was strongly congruent with synchrotron-based models. Analysis of these crystals by SFX should now enable structural characterization of the early events that drive phytochrome photoconversion

Age-related Changes in Bone Marrow Mesenchymal Stromal Cells: A Potential Impact on Osteoporosis and Osteoarthritis Development

Aging at the cellular level is a complex process resulting from accumulation of various damages leading to functional impairment and a reduced quality of life at the level of the organism. With a rise in the elderly population, the worldwide incidence of osteoporosis (OP) and osteoarthritis (OA) has increased in the past few decades. A decline in the number and “fitness” of mesenchymal stromal cells (MSCs) in the bone marrow (BM) niche has been suggested as one of the factors contributing to bone abnormalities in OP and OA. It is well recognized that MSCs in vitro acquire culture-induced aging features such as gradual telomere shortening, increased numbers of senescent cells, and reduced resistance to oxidative stress as a result of serial population doublings. In contrast, there is only limited evidence that human BM-MSCs “age” similarly in vivo. This review compares the various aspects of in vitro and in vivo MSC aging and suggests how our current knowledge on rejuvenating cultured MSCs could be applied to develop future strategies to target altered bone formation processes in OP and OA