Constructing and Medical Trials of a Monoclonal Dot-Immuno-Enzyme Test-System “DIATul-M” for Tularemia Microbe Detection

mc/ml. Additionally, this test-system has been proving for acquisition of sustainable results after 6 months of storing (the observation period). Medical trials of the panel of reagents “Monoclonal dot-immuno-enzyme test-system for tularemia microbe detection” have shown it to be a prospective preparation for implementation into the national healthcare practices both under stationary and field conditions

Сравнение экспрессии гена РСА3 в осадках и экзосомах мочи при раке предстательной железы

Introduction. Prostate cancer (PCa) is one of the common oncological diseases in men. Expression of the PCA3 gene in urine is currently used as a molecular genetic marker of PCa.Objective: to comparative analysis of the PCA3 expression in urine sediments and exosomes for the determination of the biomaterial, which allows detecting the PCA3 expression in more efficient manner.Materials and methods. The 12 patients with different stages of PCa and 8 control samples were examined.Results. The diagnostic accuracy of the PCA3 gene expression analysis in this cohort exceeded 90 %. We had not obtained significant differences in the sensitivity and specificity of the PCA3 hyperexpression in the urine sediments compared with exosomes. This result indicates in favor to using urine sediment for the PCA3 analysis as a biomaterial with less time-consuming sample preparation, although the possible advantage of exosomes for the analysis of the expression marker panels requires further studies.Введение. Рак предстательной железы (РПЖ) является одним из наиболее частых онкологических заболеваний у мужчин. В качестве молекулярно-генетического маркера РПЖ в настоящее время используют определение экспрессии гена РСА3 в моче.Цель работы – сравнительный анализ экспрессии гена РСА3 в осадках и в экзосомах мочи для определения биоматериала, позволяющего более эффективно проводить определение экспрессии этого гена.Материалы и методы. Были исследованы данные 12 пациентов с различными стадиями РПЖ и 8 контрольных образцов.Результаты. Диагностическая точность анализа экспрессии РСА3 в этой выборке превысила 90 %. Не выявлено достоверных различий чувствительности и специфичности гиперэкспрессии РСА3 при анализе осадков по сравнению с экзосомами мочи. Это указывает на целесообразность использования осадков мочи для анализа РСА3 как биоматериала с менее трудоемкой пробоподготовкой, однако возможные преимущества экзосом для анализа панелей экспрессионных маркеров требуют дальнейшего изучения

Microstructure and Properties of an Electrocontact Cu–(ZnO/TiO2) Material

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.Abstract—A copper-based electrical-contact composite material hardened by disperse zinc oxide and zinc titanate is studied by electron microscopy and energy dispersive X-ray macroanalysis. The distribution of oxide phases in the samples containing 2.5 wt % oxide nanopowder mixture in an initial charge is found to be uniform. An increase in the amount of oxides leads to an increase in their sized in sintering. A relation between the sample wear and the sample composition is obtained during laboratory tests. It is shown that the introduction of more than 2.5 wt % oxide mixture results in intense wear of the working surface of the sample and an increase in the running-in period of contacts

Microstructure and Properties of an Electrocontact Cu–(ZnO/TiO2) Material

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.Abstract—A copper-based electrical-contact composite material hardened by disperse zinc oxide and zinc titanate is studied by electron microscopy and energy dispersive X-ray macroanalysis. The distribution of oxide phases in the samples containing 2.5 wt % oxide nanopowder mixture in an initial charge is found to be uniform. An increase in the amount of oxides leads to an increase in their sized in sintering. A relation between the sample wear and the sample composition is obtained during laboratory tests. It is shown that the introduction of more than 2.5 wt % oxide mixture results in intense wear of the working surface of the sample and an increase in the running-in period of contacts