Diagnostics of Severe Fever with Thrombocytopenia Syndrome – Disease, Caused by Novel Phlebovirus

In 2009, a novel virus, named severe fever with thrombocytopenia syndrome (SFTS) virus, was isolated from a patient in China. The illness caused by this novel virus is characterized by a sudden onset of fever and respiratory or gastrointestinal disorders, followed by progressive thrombocytopenia and leucocytopenia, the case-fatality rate amounting to 6–30 %. Genomic sequencing of the isolated agent indicated that the SFTS virus constituted a new (third) group of Phlebovirus genus, Bunyaviridae family. Presently, different means for specific diagnostics of SFTS (real-time reverse transcription polymerase chain reaction, indirect fluorescent antibodies method, enzyme-linked immune-sorbent assay) are developed. Constructing of diagnostic kits, basic characteristics of methods for determination of causative agent of infection or specific antibodies against it are considered in this review

Structures and photophysical properties of 3,4-diaryl-1H-pyrrol-2,5-diimines and 2,3-diarylmaleimides

Structural features of 3,4-diaryl-1H-pyrrol-2,5-diimines and their derivatives have been studied by molecular spectroscopy techniques, single-crystal X-ray diffraction, and DFT calculations. According to the theoretical calculations, the diimino tautomeric form of 3,4-diaryl-1H-pyrrol-2,5-diimines is more stable in solution than the imino-enamino form. We also found that the structurally related 2,3 exist in the solid state in the dimeric diketo form. 3,4-Diary1-1H-pyrrol-2,5-diimines and 2,3-diarylmaleimides exhibit fluorescence in the blue region of the visible spectrum. The fluorescence spectra have large Stokes shifts. Aryl substituents at the 3,4-positions of 1H-pyrrol-2,5-diimine do not significantly affect fluorescence properties. The insertion of donor substituents into 2,3diarylmaleimides leads to bathochromic shift of emission bands with hyperchromic effect. (C) 2017 Elsevier B.V. All rights reserved

Magnetostimulated Chandges of Microhardness in Potassium Acid Phthalate Crystals

A decrease in microhardness along the (010) cleavage in potassium acid phthalate single crystals by 15--18% after the application of a permanent magnetic field was revealed for the first time. It is shown that the effect revealed is of the volume character. The role of interlayer water in the processes stimulated by a magnetic field is studied., Interlayer water plays does not cause the observed changes it only plays the part of an indicator of these changes in potassium acid phthalate crystals in a magnetic field. It is established that microhardness in the (100) plane of the crystal in an applied a magnetic field first increases by 12--15% and then remains constant in time within the accuracy of the experiment. The possibility of varying the crystal structure of potassium acid phthalate crystals by applying magnetic fields inducing rearrangement in the system of hydrogen bonds or in the defect structure is discussed.Comment: 6 pages, 7 figure

Твердофазный синтез Декамера отрицательно заряженных ПНК с псевдопептидной связью GlyψL-Glu

Solid-phase Boc-protocol and synthetic strategy of negatively charged peptide nucleic acids has been suggested. New thymine containing negatively charged PNA decamer based on L-glutamic acid and glycine was synthesized.Представлен Вос-протокол и стратегия твердофазного синтеза отрицательно заряженных ПНК. Синтезирован новый тиминсодержащий декамер отрицательно заряженных ПНК на основе L-глутаминовой кислоты и глицина

The Flow Cytometry Study of Cellular Immunity in Rhesus Monkeys after Experimental Infection with SARS CoV 2 Virus

Cellular immunity plays an important role in the pathogenesis and formation of protective immune defense against the SARS‑CoV‑2 virus.The aim of the work was to study the cellular immunity of rhesus monkeys applying flow cytometry after experimental infection with the SARS‑CoV‑2 virus.Materials and methods. Male rhesus monkeys were intranasally inoculated with the SARS‑CoV‑2 virus, Isolate B strain and hCoV-19/Russia/SP48-1226/2020 strain (abbreviated name U-2), at a dose of 5.0 lg PFU. Using flow cytometry, the levels of 21 populations/subpopulations of mononuclear cells in the peripheral blood of animals were determined before experimental infection with the pathogen and on day 14 after infection. SARS‑CoV‑2 coronavirus RNA was assessed using real-time polymerase chain reaction. Determination of the titer of virus-neutralizing antibodies to the SARS‑CoV‑2 virus in the blood sera of animals was conducted through neutralization test evaluating the ability to suppress negative colonies.Results and discussion. Infection with Isolate B strain culture has led to an increase in the relative content of total T-lymphocytes (p˂0.2), cytotoxic T-lymphocytes (p˂0.1), as well as monocytes expressing the early activation marker CD25 (p˂0.2). The decrease in levels has been observed for total B-lymphocytes (p˂0.2) and T-helper cells (p˂0.1). Infection with the U-2 strain culture revealed an increase in the relative content of monocytes expressing the early activation marker CD25 (p˂0.2). Thus, for the first time in the Russian Federation, flow cytometry was used to study the cellular immunity of rhesus monkeys before and after experimental infection with the SARS‑CoV‑2 virus. The obtained information can be used for studying the pathogenesis of SARS‑CoV‑2 infection, course, and outcome of the disease, and developing strategies for vaccination and treatment

Синтез нового аналога пептида ProGlyPro

A novel analogue of the biologically active peptide ProGlyPro containing an isosteric pseudopeptide bond instead of an amide one has been synthesized.существлен синтез нового аналога биологически активного пептида ProGlyPro, содержащего изостерическую псевдопептидную связь вместо амидной

ПЕДІАТРИЧНА ОСВІТА УКРАЇНИ У ХХІ СТОРІЧЧІ: СУЧАСНІ ВИКЛИКИ ТА ПРОБЛЕМИ МАЙБУТНЬОГО

The aim of the work – to evaluate the state of development of pediatric education in Ukraine in the beginning of the 21st century, staffing of the branch in the field of pediatrics and to develop a plan of measures to overcome the crisis phenomena in pediatric health care. The main body. The article presents data on the development of pediatric education in the early 21st century after the restoration of the educational-scientific specialty “Pediatrics” in 2017. The development of up-to-date standards for the training of pediatric doctors, based on the acquisition of future master’s degrees in pediatrics, general and professional competencies and necessary practical skills is highlighted. The current problems in the provision of pediatric health care facilities are emphasized. Conclusion. A list of measures at the sectoral and national level has been developed to restore the necessary training рediatricians for childrenʼs health care of the country and to ensure the proper methodological provision of the educational process, which may cause difficulties in providing children with quality medical care in the future.Мета роботи – оцінити стан розвитку педіатричної освіти в Україні на початку ХХІ сторіччя, кадрове забезпечення галузі за спеціальністю «Педіатрія» та розробити план заходів щодо подолання кризових явищ у педіатричній охороні здоровʼя. Основна частина. У статті наведено дані щодо розвитку педіатричної освіти на початку ХХІ сторіччя після відновлення освітньо-наукової спеціальності «Педіатрія» у 2017 р. Висвітлено розробку сучасних стандартів підготовки дитячих лікарів, що базуються на набутті майбутніми магістрами педіатрії загальних та фахових компетенцій і необхідних практичних навичок. Наголошено на теперішніх проблемах у кадровому забезпеченні педіатричних закладів охорони здоровʼя, що може викликати складнощі у забезпеченні дітей якісною медичною допомогою у майбутньому. Висновок. Розроблений перелік заходів на галузевому та загальнодержавному рівні щодо відновлення необхідних для країни обсягів підготовки дитячих лікарів та забезпечення належного методичного забезпечення навчального процесу

Immunological and biochemical changes in young children with recurrent course of respiratory infections and cytomegaloviral infection

Cytomegalovirus (CMV) infection in practically healthy children of different ages ranges from 50% to 64%. Both acute course of infection and prolonged persistance of pathogen is accompanied by immune deficiency formation that may become one of factors in charge of high level of infection morbidity in future as recurrent respiratory infections (RRI). At the same time significant spread of deficiency states in infants - deficiency of iron ion or vitamin D - is also accompanied by changes in immunological reactivity, especially when combined with intracellular infections. Purpose of the work was to study some immune and biochemical changes in young children (n=37) with recurrent respiratory diseases and cytomegaloviral infection. Materials and methods. In 51 children the CMV infection was verificated by simultaneous detection of specific anti-CMV IgG and anti-CMV IgM in blood serum and viral DNA finding by polymerase chain reaction. The observation group consisted of 37 (72.5%) children infected with CMV as well as suffering from recurrent respiratory infections. Comparison group was represented by the remaining 14 (27.5%) patients with episodic respiratory infections (ERI). In RRI group the patients with the frequent respiratory diseases with bacterial implications four and more times during the second year of life were included. The levels of serum iron and 25-hydroxy-cholecalciferol (vitamine 25(ОН)D) were detected in blood serum of all children by ELECSYS-test. In the early convalescence period after respiratory infections comparative immunograms assessment was conducted by three main components: phagocytic activity of neutrophils, the system of cellular immunity and quality of humoral immunity. In the vast majority (72.5%) of the children age up to 1yo with verified CMV infection respiratory infections of viral and bacterial etiology gained a recurrent course (p <0.05). In the early convalescence period after respiratory infection process in children with CMV and RRI the immune response is characterized by a decreasing of the functional and metabolic activity of neutrophile granulocytes (р<0,05) and imbalance of humoral immunity consisting in decreased antibody generation (IgА (р<0,05), IgМ (р<0,05), tendency to IgG decreasing). These changes are compensated by the increasing of the total number of B-lymphocytes (р<0,05). We founded a significantly lower average level of 25-hydroxy-cholecalciferol (32,37|23,41-39,95|ng/ml) in children of the observation group. In the comparison group this rate was 44,21|35,38-52,82|ng/ml, р<0,05. We also detected a correlation dependence between concentration of vitamin 25(OH)D and antiCMV IgG (r = 0,35, p <0.05), decreased concentration of each of them is clinically manifested in pathogen persistence and recurrence course of respiratory infections in children. In the patients from observation group serum iron concentration was significantly lower than the corresponding data of those children who suffered from the respiratory diseases occasionally: 4,05|3,74-5,05| mcmol/l and 8,6|4,92-11,72| mcmol/l (р<0,05) respectively. There was a slight correlation dependence between serum iron level and blood anti-CMV IgG-antibodies level (r=0,28, р<0,05). So, the fact of the important role of iron ions in the mechanisms which regulate the functional activity of CD4+-cells was confirmed (r=0,49, р<0,05)