Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией

Objective: to study the impact of the concentration of cytokine HIF-1α in the donor area on the processes of reparative regeneration in free autodermografting by split-thickness skin graft for patients with disorders of the microvasculature and to improve results of autodermografting by local stimulation of HIF-1α to prepare skin graft to hypoxia conditions.Materials and methods. The hypothesis of the influence of the local circulatory hypoxia on the concentration of HIF-1α was checked in the experiment with 18 white outbred male rats weighing 250–300 g. The microcirculation level was controlled by laser doppler flowmetry method. The indications to appraise the correctness of the hypothesis were analytical data of Enzyme Multiplied Immunoassay. This method of treatment was used in the clinic, where 18 patients with diabetic foot infections syndrome needed dermatoplastic surgery, and compared with the results of treatment of 22 patients with free autodermografting performed by traditional way.Results. It is identified the significant difference in concentration of cytokine HIF-1α in the donor skin area in preparation it for hypoxia conditions by proposed method. The experimental data are confirmed in the clinic. After the free autodermografting based on developed technique in the main group the viability of grafter was preserved on Me (Q1; Q3) 84 (78; 93) % of its area. In the control group graft acceptance is registered on Me (Q1; Q3) 72 (65; 79) % of grafter area (p = 0.012).Цель исследования – изучение влияния концентрации в донорской области цитокина HIF-1α на процессы репаративной регенерации при свободной аутодермопластике расщепленным кожным трансплантатом у больных с патологией микроциркуляторного русла и улучшение результатов аутодермопластики путем локальной стимуляции выработки HIF-1α в целях подготовки трансплантата к условиям гипоксии.Материалы и методы. В эксперименте гипотезу влияния локальной циркуляторной гипоксии на концентрацию HIF-1α проверили на 18 белых беспородных крысах-самцах массой тела 250–300 г. Уровень микроциркуляции контролировали методом лазерной допплеровской флоуметрии. Критериями, позволяющими оценить правильность гипотезы, явились данные иммуноферментного анализа. В клинике использовали предложенный способ при лечении 18 больных с синдромом диабетической стопы, требовавшим выполнения кожно-пластических операций, сравнивая с результатами лечения 22 больных, у которых свободную аутодермопластику выполняли традиционным способом.Результаты. Выявлена статистически значимая разница в концентрации цитокина HIF-1α в коже донорской области при подготовке ее к условиям гипоксии предложенным способом. Данные эксперимента подтверждены в клинике. В основной группе после выполнения свободной аутодермопластики по разработанной методике жизнеспособность трансплантата сохранялась на Me (Q1; Q3) 84 (78; 93) % его площади. В контрольной группе приживление отмечено на Me (Q1; Q3) 72 (65; 79) % площади трансплантата (р = 0,012)

Hydrological modeling of green roofs runoff by nash cascade model

Green roofs (GRs) technology has gained increasing interest in recent years since it offers multiple benefits to urban environments, citizens and buildings. Eco-covers can capture some water nutrient pollutants, filter air pollutants and moderate the urban heat island effect. Beyond these benefits, abundant literature stresses the role played by the GRs from the hydrological perspective. They allow to face the increasing stress on the traditional urban drainage systems by reducing the annual stormwater runoff. In light of this, the hydrological behavior prediction of a vegetated cover is essential for urban planners, policy makers and engineers in order to quantify runoff mitigation potential so as to optimize their application. Objective: The aim of the present research is to meet this need by testing the accuracy of Nash cascade model in predicting the stormwater production of GR systems. Materials and Methods: The proposed model has been calibrated against hourly data of thirteen rainfall-runoff events observed at two experimental sites, both located within the campus of the University of Salerno, southern Italy. Event scale model calibration, aimed at the identification of the storage coefficient parameter, has been based on the optimization of a number of error statistics. Results and Conclusion: Despite its simplicity, the investigated model appears adequately to be able to predict the runoff production from the experimental green roofs with a good degree of accuracy, as described by the Nash-Sutcliffe Efficiency index, which ranges between 0.54 and 0.94

New data on mammoth fauna mammals in the central Lena River basin (Yakutia, Lenskie Stolby National Nature Park and adjacent areas)

This paper considers the data on new findings of mammoth fauna remains in the Middle Lena basin used to specify the species composition of large Late Neopleistocene mammals represented by eleven species. The obtained range of radiocarbon dates made it possible to state that mass burials of Pleistocene mammal remains were formed in the region during the Karginsk Interstadial (24 000-55 000 years ago)

A study of a frozen mummy of a wild horse from the Holocene of Yakutia, East Siberia, Russia

The paper presents a description of a rare finding of the partial frozen corpse of the Yukagir horse. Discovered from thawing deposits in northern East Siberia (Yakutia, Russia), its age is confirmed to be Mid-Holocene (about 4600 BP). The mummy had a preserved head with the neck and the back of the torso with the legs and tail. The Yukagir horse was relatively short, with short ears and tail. Compared to the modern breeds, including the Yakutian domestic horse, wild Przewalski's horse, and extinct Lena horse, Equus lenensis, the Yukagir horse was closest to the latter, which was also confirmed by studies of the hair microstructure. The pollen and plant remains from the horse's intestines indicated a preference to grasses. The late geological age of the Yukagir horse is an indication that this species survived the Pleistocene-Holocene crisis and lived through the Mid-Holocene in northern Eastern Siberia

New Data on Large Brown Bear (Ursus arctos L., 1758, Ursidae, Carnivora, Mammalia) from the Pleistocene in Yakutia

New finds of brown bear (Ursus arctos L., 1758) fossil remains from the territory of Yakutia, namely, skulls and mandibular bones, have been investigated. The new finds are exceptionally large: most dimensions of these specimens exceed those of the present-day brown bears of Yakutia and even the maximal parameters of the largest individuals of the present-day Eurasian subspecies U. a. beringianus and U. a. piscator. Analysis of various data showed that giant brown bears had inhabited northern Yakutia during the Karginian interstadial in the Late Pleistocene