33 research outputs found
Development and validation of a method for determination of Eculizumab concentration in human plasma by biolayer interferometry
The article describes a new method for measurement of monoclonal antibody Eculizumab concentration in human plasma in a range of 3-250 ΞΌg/ml. In this method we used an antibody Fab fragment, capable of binding to eculizumab specifically in human plasma. The method uses the biolayer interferometry for determination of Eculizumab concentration without additional tag in the analyzing substances. This method was comparatively validated along with the traditional ELISA. Comparative validation demonstrated that the biolayer interferometry based method has an advantage to the ELISA in such important statistical criteria as an analytical range, accuracy, precision, specificity and selectivity (matrix effect)
Spectroscopy of superheavy hydrogen isotopes
The superheavy hydrogen isotopes 4H and 5H have been investigated in the stopped pion absorption on 9Be. Three states of 4H were proposed in the reaction channel 9Be( Ο{ - }, dt)X. Four states of 5H were proposed in the reaction channels 9Be(Ο{ - }, pt)X and 9Be(Ο{ - }, dt)X. The excited states of 5H can decay into free nucleons
Search for superheavy hydrogen isotopes
Experimental search for superheavy hydrogen isotopes 6H and 7H was performed in the stopped pion absorption on 9Be and 11B nuclei. The structures in the missing-mass spectra were observed in the reaction channels 9Be(Ο-, pd )X and 11B(Ο-, p4He)X . Four states of 6H were proposed. Evidences for 7H formation were obtained in the reaction channels 9Be(Ο-, pp)X and 11B(Ο-, p3He)X
Π Π°Π·ΡΠ°Π±ΠΎΡΠΊΠ° ΠΈ Π²Π°Π»ΠΈΠ΄Π°ΡΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΡΠΊΡΠ»ΠΈΠ·ΡΠΌΠ°Π±Π° Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅Ρ Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ Π±ΠΈΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠΌΠ΅ΡΡΠΈΠΈ
The article describes a new method for measurement of monoclonal antibody Eculizumab concentration in human plasma in a range of 3-250 ΞΌg/ml. In this method we used an antibody Fab fragment, capable of binding to eculizumab specifically in human plasma. The method uses the biolayer interferometry for determination of Eculizumab concentration without additional tag in the analyzing substances. This method was comparatively validated along with the traditional ELISA. Comparative validation demonstrated that the biolayer interferometry based method has an advantage to the ELISA in such important statistical criteria as an analytical range, accuracy, precision, specificity and selectivity (matrix effect).Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΠΈ Π²Π°Π»ΠΈΠ΄ΠΈΡΠΎΠ²Π°Π½Π° ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½ΡΠΈΡΠ΅Π»Π° ΡΠΊΡΠ»ΠΈΠ·ΡΠΌΠ°Π± Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ 3-250 ΠΌΠΊΠ³/ΠΌΠ». ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΎΡΠ½ΠΎΠ²Π°Π½Π° Π½Π° Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΈ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Fab-ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° Π°Π½ΡΠΈΡΠ΅Π»Π°, ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΠ³ΠΎ ΠΊ ΡΠΊΡΠ»ΠΈΠ·ΡΠΌΠ°Π±Ρ Π² ΠΎΠ±ΡΠ°Π·ΡΠ΅ ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ. ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅Ρ ΠΏΡΠΈΠ½ΡΠΈΠΏ Π±ΠΈΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠΌΠ΅ΡΡΠΈΠΈ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠΈΠΉ Π±Π΅Π· Π²Π½Π΅ΡΠ΅Π½ΠΈΡ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΌΠ΅ΡΠΎΠΊ ΠΎΡΠ΅Π½ΠΈΡΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΡΠ΅ΠΌΠΎΠ³ΠΎ Π²Π΅ΡΠ΅ΡΡΠ²Π°. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π²Π°Π»ΠΈΠ΄Π°ΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΡΠΎΠΏΠΎΡΡΠ°Π²Π»ΡΠ»ΠΈΡΡ Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°ΠΌΠΈ Π²Π°Π»ΠΈΠ΄Π°ΡΠΈΠΈ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. ΠΠΎ ΠΈΡΠΎΠ³Π°ΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²ΠΎ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π±ΠΈΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠΌΠ΅ΡΡΠΈΠΈ ΠΏΠΎ ΠΎΡΠ½ΠΎΠ²Π½ΡΠΌ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°ΠΌ - Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌΡ Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Ρ, ΠΏΡΠ°Π²ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ, ΠΏΡΠ΅ΡΠΈΠ·ΠΈΠΎΠ½Π½ΠΎΡΡΠΈ ΠΈ ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ