132 research outputs found
Važnija eksterijerna i reproduktivna svojstva populacije engleskog punokrvnog konja gajenog na ergeli 'LjubiÄevo', Srbija
The investigation included 5 stallions (average age of 10.40 years) and 33 mares (average age of 10.33 years) with pedigree of the English Thoroughbred horse breed that are used for breeding on the Stud Farm Ljubicevo - Serbia. The investigation of some significant properties relating to the exterior (at stallions) and reproductive ones (at mares) were carried out in the year 2009. The following average exterior (body) measures of the stallions: the body mass (474.80 kg), withers height (160.46 cm), trunk or body length (161.88 cm), breast circumference (187.96 cm) and tibia circumference (19.22 cm) were within the standards of this horse breed of the age. The gestation period at mares, regardless the sex of a colt, averagely lasted 337.70 days. With the mares having had a male colt, the gestation lasted a little bit longer (338.92 days) than with the mares having had female colts (336.90 days). The difference in the gestation duration (2.02 days) was not statistically significant (P>0.05). Between the age of the mares and the gestation duration (regardless the sex of a colt) it was found a positive slight correlation (rp=0.320). Furthermore, between the age of the mares and the gestation duration it was found medium (rp=0.453) correlation at male colts, and quite poor correlation (rp=0.202) at female colts. Found coefficients of the phenotype correlation were not statistically confirmed (P>0.05).Istraživanjima je obuhvaÄeno 5 pastuva (proseÄne starosti 10,40 godina) i 33 kobile (proseÄne starosti 10,33 godina) sa poznatim poreklom (pedigreom) engleske punokrvne rase konja koja se na ergeli LjubiÄevo - Srbija aktivno koriste u priplodu. UtvrÄivanja važnijih eksterijernih (pastuvi), odnosno reproduktivnih svojstava (kobile) sprovedena su u 2009. godini. UtvrÄene proseÄne eksterijerne (telesne) mere pastuva: telesna masa (474,80 kg), visina grebena (160,46 cm), dužina trupa - tela (161,88 cm), obim grudi (187,96 cm) i obim cevanice (19,22 cm) su bile u okviru standarda ove rase konja u odgovarajuÄoj starosnoj dobi. Bremenitost (gestacija) kobila, bez obzira na pol ždrebeta, u proseku je trajala 337,70 dana. Kod kobila koje su oždrebile muÅ”ku ždrebad bremenitost je trajala neÅ”to duže (338,92 dana), nego kod kobila koje su oždrebile žensku ždrebad (336,90 dana). Razlika u trajanju bremenitosti (2,02 dana) nije bila statistiÄki signifikantna (P>0,05). IzmeÄu starosti kobila i trajanja bremenitosti (bez obzira na pol ždrebeta) utvrÄena je pozitivna slaba korelaciona povezanost (rp=0,320). Zatim, izmeÄu starosti kobila i trajanja bremenitosti utvrÄena je srednja (rp=0,453) korelaciona povezanost kod muÅ”ke ždrebadi, odnosno jako slaba (rp=0,202) kod ženske ždrebadi. UtvrÄeni koeficijenti fenotipske korelacije nisu bili statistiÄki potvrÄeni (P>0,05)
Dobijanje i karakterizacija dva tipa imobilizovane amiloglukozidaze
Amyloglucosidase from A. niger was covalently immobilized onto poly(GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined.Amiloglukozidaza iz A.niger je imobilizovana na poly(GMA-co-EGDMA) glutaraldehidnom i perjodatnom metodom. Imobilizacija amiloglukozidaze nakon perjodatne oksidacije daje preparat sa najveÄom do sada objavljenom specifiÄnom aktivnosti na sliÄnim polimerima. Dobijeni imobilizovani preparat ima isti pH optimum ali poveÄani termooptimum u poreÄenju sa rastvornim enzimom. OdreÄeni su i kinetiÄki parametri za hidrolizu rastvornog skroba imobilizovanim kao i rastvornim enzimom
Izvori varijabilnosti osobina porasta i telesne razvijenosti bikova Simentalske rase u performans testu
To test the variability of traits of Simmental bulls in performance test, data of the Livestock - Veterinary Centres for Reproduction and Artificial Insemination of Velika Plana and KrnjaÄa were used. In the analysis, data on 113 performance tested bulls born from 2008 to 2009 were used. The analysis included two sets of characteristics: body development traits and growth traits. The average body mass of calves entering the test was 195.75 kg, while the body mass at the end of the test was 476.50 kg, average daily gain in the test was 1138.69 g. Average values of body development traits measured at the end of the test, with 12 months of age were: height at withers 127.13 cm, chest circumference 179.42 cm, the chest depth 61.19 cm and body length 151.34 cm. The influence of their sires, the year and the Centre on the variability of traits was studied. The effect of age is present at a high level of statistical significance (p lt 0.01) for all traits that are registered at the end of the test, while the effect of the Centre was present in the variability of body mass at the end of the test, the daily gain in the test and the length of the body. The bulls-sires' influence was demonstrated (p lt 0.05) on the variability in body mass of calves entering the test.Za ispitivanje varijabilnosti osobina simentalskih bikova u performans testu iskoriÅ”Äeni su podaci stoÄarsko-veterinarskog centra za reprodukciju i veÅ”taÄko osemenjavanje iz Velike Plane i KrnjaÄe. Za analizu su upotrebljeni podaci o 113 performans testiranih bikova roÄenih u periodu od 2008 do 2009 godine. Analizom su obuhvaÄene dve grupe osobina: osobine telesne razvijenosti i osobine porasta. ProseÄna telesna masa sa kojom su telad ulazila u test iznosila je 195,75 kg, dok je telesna masa na kraju testa 476,50 kg, proseÄan dnevni prirast u testu iznosio je 1138,69 g. ProseÄne vrednosti osobina telesne razvijenosti merenim na kraju testa, sa 12 meseci uzrasta iznosile su: visina grebena 127.13 cm, obim grudi 179.42 cm, dubina grudi 61.19 cm i dužina trupa 151.34 cm. Analizirani su uticaj oÄeva, godine i centra na varijabilnost osobina. Efekat godine je prisutan na visokom nivou statistiÄke znaÄajnosti (p lt 0,01) za sve osobine koje se registruju na kraju testa, dok je efekat centra prisutan u varijabilnosti telesne mase na kraju testa, dnevnog prirasta u testu i dužini tela. Bikovi-oÄevi su ispoljili uticaj (p lt 0,05) na varijabilnost telesne mase sa kojom su telad ulazila u test
Hemijske modifikacije Ī²-laktoglobulina hinonima
The avarone/avarol quinone/hydroquinone couple. as well as their derivatives show considerable antitumor activity. In this work, covalent modifications of beta-lactogglobulin. isolated from cow milk by avarone, its model compound 2-tert-butyl-1,4-benzoquinone. and several of their alkylthio derivatives were studied. The techniques applied for as-saying the modifications were: UV/VIS spectrophotometry, SDS PAGE and isoelectrofocusing. The results of the SDS PAGE suggest that polymerisation of the protein occurs. The shift of the pI of the protein upon modification toward lower values indicates that lysine amino groups are the principal site of die reaction of beta-lactoglobulin with the quinones.Hinonsko/hidrohinonski par avaron/avarol i njihovi derivati pokazuju znaÄajnu antitumorsku aktivnost. U ovom radu prouÄavane su kovalentne modifikacije Ī²-laktoglobulina, izolovanog iz kravljeg mleka, avaronom, njegovim model-jedinjenjem 2-tert-butil-1,4-benzohinonom i njihovim alkiltio-derivatima. Za ispitivanje modifikacija koriÅ”Äene su UV/VIS spektrofotometrija, SDS PAGE i izoelektrofokusiranje. Rezultat SDS PAGE ukazuje da se protein polimerizuje. Pomeranje pI vrednosti proteina nakon modifikacije ka nižim vrednostima pokazuje da su amino grupe lizina glavna mesta reakcije Ī²-laktoglobulina sa hinonima
Životna proizvodnja visokomleÄnih krava
Lifetime milk production is a key success factor in fulfilling the production potential of high-yielding cows. Lifetime milk production traits are pronouncedly variable. The life expectancy and the length of productive life of dairy cows are repeatedly limiting factors for improving lifetime milk production. Lifetime milk production is greatly depended on age at first calving and the number of lactations during productive life. Previous researches have implied there are real chances for improving the lifetime milk production of high-yielding cows. The goal of this research was to investigate the significance of key systematic factors on the lifetime production of high-yielding Black-and-White cows. The animals included in the sample had different share of Holstein genes. The researchers determined systematic factors that caused some significant phenotypic variations of the investigated trait. The average lifetime milk production was 25,002.66Ā±7,755.39 kg. When observed by cow genotypes, the mean values of the lifetime milk production varied from 27,061.37 kg ( (lt) 58% HF) and 24,761.26 kg (58-73% HF) to 23,185.36 kg (>73% HF). The differences in lifetime milk production determined among the animals were due to a highly significant (pā¤0.01) impact of the bulls - the sires of the cows and the year of culling; the impact of the class of HF genes was significant (pā¤0.05), whereas the impact of the reason for culling was non-significant (p>0.05).Osobine životne proizvodnje visokomleÄnih krava imaju veliki ekonomski znaÄaj. Crno-bela goveda imaju znaÄajan genetski potencijal za proizvodnju mleka. Visokoproizvodne krave crno-bele rase izložene su tokom produktivnog veka velikom broju složenih uticaja koji intenzivno deluju na nivo relizacije njihovog genetskog potencijala. Uslovi sredine Äesto nisu u saglasnosti sa potrebama ove visokomleÄne rase goveda. PoveÄanjem uÄeÅ”Äa gena holÅ”tajn-frizijske rase, tokom procesa oplemenjivanja evropskog tipa crno-belih goveda, doÅ”lo je do poveÄanja prinosa mleka. DosadaÅ”nja istraživanja ukazuju na moguÄnost za znaÄajnije poveÄanje životne produktivnosti crno-belih krava. Poznavanje broja i nivoa uticaja faktora sredine, na životnu proizvodnju visokomleÄnih krava, važno je zbog njihovog ukljuÄivanja u model. U skladu sa znaÄajem pojedinih sistematskih faktora u okviru istraživanja je obavljena njihova objektivna procena. Cilj rada je bio da se primenom odgovarajuÄe metodologije ispita znaÄajnost razlika, u ostvarenoj životnoj proizvodnji mleka kod visokoproizvodnih crno-belih krava, preko najvažnijih sistematskih uticaja. ProseÄna životna proizvodnja mleka iznosila je 25002.66Ā±7755.39 kg litara. Posmatrano po genotipovima krava proseÄne vrednosti životne proizvodnje mleka iznosile su 27061.37 kg ( (lt) 58% HF), 24761.26 kg (58-73% HF) i 23185.36 kg (> 73% HF). UtvrÄene razlike izmeÄu grla u pogledu ostvarenog nivoa životne proizvodnje mleka nastale su kao posledica visoko znaÄajnog uticaja (Pā¤0.01) bikova-oÄeva krava i godine izluÄenja krava, klasa HF gena krava imala je znaÄajan uticaj (pā¤0.05), dok razlog izluÄenja nije imao znaÄajan uticaj (P>0.05)
Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Cotinus Coggygria Scop. Against Breast and Colon Cancer Cell Lines
Background and objective: Cotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types ā breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26). Materials and methods: Cell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fl uorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line. Results: The obtained results showed that fustin reduced the proliferation of all studied cell lines. The highest cytostatic eff ect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 Ī¼g/ ml followed by colon cancer cells with an IC50 of 78.07 Ī¼g/ml. MCF7 cell proliferation was least aff ected with a calculated IC50 of 187.8 Ī¼g/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell monolayer and some morphological alterations, signifi cant attenuation in the number of viable cells, and diminished clonogenic ability of cells after fustin exposure. Conclusion: It could be concluded that fustin isolated from the heartwood of medicinal plant C. coggygria possesses marked antiproliferative properties against breast cancer cell line MDA-MB-231 which will be a subject of our more detailed future investigations
Schizophyllum commune: The main cause of dying trees of the Banja Luka arbored walks and parks
In the frame of investigation of the main cause of dying trees of the main arbored walks (Mladena StojanoviÄa Aley and Park), the investigation of the presence and diversity of macrofungi in Banja Luka City were undertaken in the period 2006-2011. Relatively poor generic diversity of lignicolous (pathogenic or potentially pathogenic and saprotrophic) macrofungi with only 16 species representing this group (13 basidiomycets: Schizophyllum commune, Fomes fomentarius, Stereum hirsutum, Coriolus versicolor, Flammulina velutipes, Pseudotrametes gibbosa, Ganoderma applanatum, G. lucidum, G. adspersum, Polyporus squamosus, Meripilus giganteus, Laetiporus sulphureus, AuricuĀlaria auricula-judae, and 3 ascomycets: Nectria cinnabarina, Xylaria hypoxylon, X. polyĀmorpha) were recorded. Such a poor qualitative composition of this very important fungal group could be explained by the reduction in the number of plant species in arbored walks and alleys, as well as the reduction in the number of fungi resistant to heavy air pollution caused by nearby (1-5m) fuel combustion in engines. Although only preliminary, our results pointed to the necessity of conservation and protection of the most beautiful features of Banja Luka and its alleys and arbored walks, by undertaking the measures of curing damaged trees and treating them with fungicides in order to wipe out the epiphytia caused in more than 95% of cases (dated May 2011) by Split-gill (Schizophyllum commune), present on dead wood but also on damaged trees of Aesculus hyppocastaneum (127 trees), Tilia cordata (124 trees), Tilia platyphyllos (36 trees), Tilia argentea (40 trees), Acer negundo (20 trees), Platanus acerifolia (2 trees), Robinia pseudoacacia (3 trees), Fraxinus ornus (1 tree), Betula pendula (1 tree), Catalpa sp. (2 trees), etc. Altogether, during the last decade, around 200 trees collapsed or were sanitary cut in Banja Luka arbored walk from the Malta site to the Green bridge, a total length around 5 km. The reason for this was primarily due to Split-gill fungus and the restoration of arbored walks in the streets extremely polluted by engine fuel consumption in the zone of Mladena StojanoviÄa street. By analyzing the trees along the City of Banja Luka main street it could be concluded that, besides the appearance of suffocation of plants, due to wide asphalt surfaces that are located immediately next to the tree-trunks and heavy air pollution, fungi caused illnesses are the most important cause of the decline of trees. With its great adaptation to arid climate and ability to resist to the air pollution, Schizophyllum commune turned out to be the most aggressive and successful universal fungal invader of trees from old alleys, even threatening immunoĀcompromised human individuals. However, man and his direct or indirect impacts contribĀute to the dying of trees much faster than the fungal pathogens in the busiest and most polluted Mladena StojanoviÄa street
Uticaj sastava i udela inertne komponente na morfologiju i parametre porozne strukture makroporoznog kopolimera 4-vinilpiridina i etilenglikoldimetakrilata
In this study, the influence of the type and content of inert component in the reaction mixture on the properties of crosslinked macroporous copolymers of 4-vinyl pyridine and ethylene glycol dimethacrylate, poly (4-VP-co-EGDMA), synthesized by suspension copolymerization was studied. n-Heptane, n-octane, n-nonane, octanol and dodecanol were used the inert component. The porosity parameters of the synthesized copolymers were determined by mercury porosimetry. Morphology of copolymer particles was characterized by scanning electron microscopy. It was shown that all the synthesized poly (4-VP-co-EGDMA) samples have globular structure. Additionaly, our results showed that it was possible to influence the size of the globules, their agglomeration and spatial arrangements and also the porosity parameters of the synthesized copolymers by the changing type and ratio of the inert component in the reaction mixture.U okviru ovoga rada prikazani su rezultati dobijeni izuÄavanjem uticaja vrste i udela inertne komponente u reakcionoj smeÅ”i na svojstava umreženog makro-poroznog kopolimera 4-vinilpiridina i etilenglikoldimetakrilata, poli(4-VP-co-EGDMA), sintetizovanog suspenzionom kopolimerizacijom. U svojstvu inertne komponente koriÅ”Äeni su: n-heptan, n-oktan, n-nonan, oktanol i dodekanol. Parametri porozne strukture sintetizovanih kopolimera su odreÄivani metodom živine porozimetrije, a morfologija pomoÄu skenirajuÄeg elektronskog mikroskopa. Pokazano je da svi sintetizovani uzorci poli(4-VP-co-EGDMA) imaju globularnu strukturu i da se izmenom vrste i udela inertne komponente u reakcionoj smeÅ”i može uticati na veliÄinu globula, njihovu aglomeraciju i raspodelu aglomerata u prostoru, a samim tim i na parametre porozne strukture sintetizovanih kopolimera
Parcijalna karakterizacija antibakterijskog peptida koji proizvodi probiotska bakterija Lactobacillus plantarum G2
The aim of this study was the partial characterization of the antimicrobial peptide bacteriocin G, produced by probiotic bacteria Lactobacillus plantarum G(2), which was isolated from a clinical sample of a healthy person. Antimicrobial substance was secreted in the supernatant of an L. plantarum G(2) culture, and showed a diverse spectrum of antimicrobial activity of all the tested strains of the genera Lactobacillus and the pathogenic bacteria Staphylococcus aureus and Salmonella abony. Isoclectric focusing revealed that bacteriocin G(2) is a cationic peptide (pI about 10) with a molecular mass of 2.2 kDa according to tricine sodium dodecyl sulphate polyacrylamide gel electrophoresis, SDS-PAGE. The antimicrobial activity of bactcriocin G(2) was diminished by the proteolytic action of trypsin and proteinase K. Bacteriocin G(2) preserved its biological activity in the temperature range 40-60 degrees C (15 min), which was lost at 80 degrees C. Bacteriocin G(2) was stable in the pH range 2-9, while treatment with 1 % Tween 80 and 1 % urea resulted in increased antimicrobial activity. The probiotic strain L. plantarum G(2) produces the antimicrobial substance proteinaceous in nature with bacteriocin characteristics. Bacteriocin production is one of the key properties of probiotic bacteria with clinical potential as anti-infective agents, which will increase the likelihood of its in vivo efficacy.Cilj ovog istraživanja je bila parcijalna karakterizacija antimikrobnog peptida iz probiotske bakterija Lactobacillus plantarum G2 izolovane iz kliniÄkog uzorka zdrave osobe. Antimikrobno jedinjenje iz L. plantarum G2, oznaÄeno kao bakteriocin G2, dobijeno iz supernatanta bakterijske kulture, pokazalo je Å”irok spektar antimikrobne aktivnosti, inhibirajiÄi rast svih ispitivanh vrsta roda Lactobacillus, kao i patogenih bakterija Staphylococcus aureus i Salmonella abony. Bakteriocin G2 je osetljiv na proteolitiÄko dejstvo tripsina i proteinaze K. Antimikrobna aktivnost je stabilna u opsegu 40-60Ā°C (15 min), ali se gubi na temperaturi od 80Ā°C. Ustanovljeno je da je bakteriocin stabilan na pH vrednostima izmeÄu 2 i 9. Dejstvo Tween-a 80 i uree je dovelo do poveÄane inhibitorne aktivnosti. Prema IEF, bakteriocin G2 je katjonski protein, sa pI vrednoÅ”Äu oko 10, a molekulaska masa odreÄena na osnovu tricin-SDS-PAGE je 2,2 kDa. Probiotski soj L plantarum G2 produkuje antimikrobno jedinjenje proteinske strukture sa karakteristikama bakteriocina. Sinteza bakteriocina je jedna od kljuÄnih osobina probiotskih bakterija koje imaju kliniÄki potencijal kao antiinfektivni agensi, jer znaÄajno poveÄava verovatnoÄu njihove in vivo efikasnosti
Ameliorative effects of the flavonoid fustin in a rat model of trinitrobenzensulfonic acid-induced colitis
Trinitrobenzene sulfonic acid (TNBS)-induced colitis is a widely used animal model that mimics the signs and symptoms of inflammatory bowel disease. Fustin is a flavonoid found in Cotinus coggygria. The aim of the present study was to investigate the effect of fustin isolated from Cotinus coggygria heartwood in a rat model of TNBS-induced colitis. In this experiment, 30 male Wistar rats were used, allocated to three groups: Control, TNBS and TNBS+F10. Colitis was induced by rectal application of TNBS. After the induction of colitis, fustin at a dose of 10 mg/kg was administered orally to TNBS+F10 group in the course of 8 days. Severity of colitis, oxidative stress and inflammation were assessed by macroscopic, histopathological, immunohistochemical and biochemical analyses. Rats from TNBS group demonstrated severe colonic damage. Fustin treatment ameliorated most of the macroscopic and some of the histopathological indices of colonic damage, and restored the activity of the endogenous antioxidant superoxide dismutase in tissue homogenate but did not affect the signs of inflammation measured by the activity of alkaline phosphatase in serum and tissue homogenate, as well as the expression of NF-kB in the colon. In conclusion, the ameliorative effects of fustin in the experimental TNBS-induced colitis might be the result of its antioxidant properties.International Conference: Kliment's Days 2023 - 60 years Faculty of Biology 09/11/2023 - 11/11/2023 Sofia, Bulgari
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