Phenomenological characteristic of the electron component in gamma-quanta initiated showers

The phenomenological characteristics of the electron component in showers initiated by primary gamma-quanta were analyzed on the basis of the Tien Shan experimental data. It is shown that the lateral distribution of the electrons ion gamma-quanta initiated showers can be described with NKG - function with age parameters bar S equals 0, 76 plus or minus 0, 02, different from the same parameter for normal showers with the same size bar S equals 0, 85 plus or minus 0, 01. The lateral distribution of the correspondent electron energy flux in gamma-quanta initiated showers is steeper as in normal cosmic ray showers

Solar cosmic ray bursts and solar neutrino fluxes

The neutrino flux detected in the C1-Ar experiment seems to respond to the powerful solar cosmic ray bursts. The ground-based detectors, the balloons and the satellites detect about 50% of the bursts of soalr cosmic ray generated on the Sun's visible side. As a rule, such bursts originate from the Western side of the visible solar disk. Since the solar cosmic ray bursts are in opposite phase withthe 11-year galactic cosmic ray cycle which also seems to be reflected by neutrino experiment. The neutrino generation in the bursts will flatten the possible 11-year behavior of the AR-37 production rate, Q, in the Cl-Ar experiment. The detection of solar-flare-generated gamma-quanta with energies above tens of Mev is indicative of the generation of high-energy particles which in turn may produce neutrinos. Thus, the increased Q during the runs, when the flare-generated high energy gamma-quanta have been registered, may be regarded as additional evidence for neutrino geneation in the solar flare processes

Search for excess showers from Crab Nebula

The arrival directions of muon poor showers registrated in the Tien Shan experiment during an effective running time about I,8.IO(4)h were analyzed. It is shown that there is a significant excess of these showers coming the direction of Crab Nebula

X-ray film chamber with carbon target of Tien-Shan complex array

X-ray films were exposed inside the ionization calorimeter under 74g/sq cm of carbon and 5 cm of lead. The X-ray film chamber area is 35 sq. m. Moving X-ray films were used, 50% of the events, which succeeded to determine incidence time, were identified with corresponding extensive air showers (EAS). For such events the size spectrum of associated EAS was derived. Two methods of energy measurement using X-ray films and ionization calorimeter were compared. The energy transfer from selected hadron to electromagnetic component is illustrated. It is found that in cascades with high energy release into electromagnetic components the hadron component is practically absent

Recycling of epidermal growth factor-receptor complexes in A431 cells: identification of dual pathways

The intracellular sorting of EGF-receptor complexes (EGF-RC) has been studied in human epidermoid carcinoma A431 cells. Recycling of EGF was found to occur rapidly after internalization at 37 degrees C. The initial rate of EGF recycling was reduced at 18 degrees C. A significant pool of internalized EGF was incapable of recycling at 18 degrees C but began to recycle when cells were warmed to 37 degrees C. The relative rate of EGF outflow at 37 degrees C from cells exposed to an 18 degrees C temperature block was slower (t1/2 approximately 20 min) than the rate from cells not exposed to a temperature block (t1/2 approximately 5-7 min). These data suggest that there might be both short- and long-time cycles of EGF recycling in A431 cells. Examination of the intracellular EGF-RC dissociation and dynamics of short- and long-time recycling indicated that EGF recycled as EGF-RC. Moreover, EGF receptors that were covalently labeled with a photoactivatable derivative of 125I-EGF recycled via the long-time pathway at a rate similar to that of 125I-EGF. Since EGF-RC degradation was also blocked at 18 degrees C, we propose that sorting to the lysosomal and long-time recycling pathway may occur after a highly temperature-sensitive step, presumably in the late endosomes

Acetylcholine-induced inhibition of presynaptic calcium signals and transmitter release in the frog neuromuscular junction

© 2016 Khaziev, Samigullin, Zhilyakov, Fatikhov, Bukharaeva, Verkhratsky and Nikolsky.Acetylcholine (ACh), released from axonal terminals of motor neurons in neuromuscular junctions regulates the efficacy of neurotransmission through activation of presynaptic nicotinic and muscarinic autoreceptors. Receptor-mediated presynaptic regulation could reflect either direct action on exocytotic machinery or modulation of Ca2+ entry and resulting intra-terminal Ca2+ dynamics. We have measured free intra-terminal cytosolic Ca2+ ([Ca2+]i) using Oregon-Green 488 microfluorimetry, in parallel with voltage-clamp recordings of spontaneous (mEPC) and evoked (EPC) postsynaptic currents in post-junctional skeletal muscle fiber. Activation of presynaptic muscarinic and nicotinic receptors with exogenous acetylcholine and its non-hydrolized analog carbachol reduced amplitude of the intra-terminal [Ca2+]i transients and decreased quantal content (calculated by dividing the area under EPC curve by the area under mEPC curve). Pharmacological analysis revealed the role of muscarinic receptors of M2 subtype as well as d-tubocurarine-sensitive nicotinic receptor in presynaptic modulation of [Ca2+]i transients. Modulation of synaptic transmission efficacy by ACh receptors was completely eliminated by pharmacological inhibition of N-type Ca2+ channels. We conclude that ACh receptor-mediated reduction of Ca2+ entry into the nerve terminal through N-type Ca2+ channels represents one of possible mechanism of presynaptic modulation in frog neuromuscular junction

Calcium Transient and Quantal Release in Mouse Neuromuscular Junction Under Extracellular Calcium Concentration Change

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. In mouse neuromuscular junction, the amplitude of the presynaptic calcium (Ca2+) transient was measured and correlated with mediator release at different extracellular Ca2+ concentrations. Fluorescent calcium-sensitive dye Oregon Green 488 BAPTA 1 hexapotassium salt was used for Ca2+ transient registration. The quantal content of release was assessed by the amplitude of the endplate potentials (EPPs) and was measured using intracellular microelectrodes. The amplitude of the EPPs changed more significantly than the amplitude of the Ca2+ transient when the extracellular calcium concentration was changed. Linear approximation of the dependence of the quantal content on the amplitude of the Ca2+ transient on double logarithmic scale gave a slope showing that the biochemical cooperativity was 2.86. The obtained value is comparable with the data calculated earlier in the neuromuscular junction of the rat and other synapses using electrophysiological measurements. Our data suggest that the change of the Ca2+ transients recorded from the whole volume of the nerve terminal properly reflects the variation of calcium concentration responsible for the neurotransmitter release in active zone. Thus, analysis of the bulk Ca2+ transient can be used to evaluate the calcium entry into the nerve endings and compare it with the number of quanta released under different conditions

Loading a calcium dye into frog nerve endings through the nerve stump: Calcium transient registration in the frog neuromuscular junction

© 2017 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. One of the most feasible methods of measuring presynaptic calcium levels in presynaptic nerve terminals is optical recording. It is based on using calcium-sensitive fluorescent dyes that change their emission intensity or wavelength depending on the concentration of free calcium in the cell. There are several methods used to stain cells with calcium dyes. Most common are the processes of loading the dyes through a micropipette or pre-incubating with the acetoxymethyl ester forms of the dyes. However, these methods are not quite applicable to neuromuscular junctions (NMJs) due to methodological issues that arise. In this article, we present a method for loading a calcium-sensitive dye through the frog nerve stump of the frog nerve into the nerve endings. Since entry of external calcium into nerve terminals and the subsequent binding to the calcium dye occur within the millisecond time-scale, it is necessary to use a fast imaging system to record these interactions. Here, we describe a protocol for recording the calcium transient with a fast CCD camera