Isolation and Characterization of Plant growth-promoting Endophyticdiazotrophic Bacteria from Sri Lankan Rice Cultivars and Rapid Screening for their effect on Plant Growth Promotion

The present study was conducted to isolate and identify endophyticdiazotrophic bacteria in two Sri Lankan rice (Oryza Sativa L.) varieties; Suwandel and Bg 358 and to evaluate their potential to promote rice plant growth. A total of 15 putative endophyticdiazotrophic bacterial isolates were obtained from shoots and roots of Suwandel and Bg 358 rice varieties out of which 7 isolates were selected based on their ability to produce IAA and phosphate solubilization. According to the morphological characters and biochemical tests, these bacteria were identified belong to genera Bacillus (IN003, IN006, and IN007), Klebsiella (IN008 and IN018), Pantoea (IN009), and Enterobacter (IN015). All selected bacterial isolates produced IAA (7.1µmgl-1 to 30.9 µ mgl-1) in the tryptophan supplemented medium. Five out of seven bacterial isolates (IN006, IN007, IN008, IN015, and IN018) were able to solubilize inorganic phosphate on Pikovskaya\u27s agar medium. Rice seeds (Suwandel variety) treated with these endophyticdiazotrophic bacteria with plant growth-promoting ability showed significantly enhanced shoot length, root length, shoot fresh weight, shoot dry weight and root fresh weight compared to the uninoculated control. Plant inoculation experiment indicated that Enterobacter sp. (IN015) was most effective in rice plant growth promotion among seven bacterial isolates tested. These results strongly suggest that endophyticdiazotrophic bacteria characterized in this study could be successfully used to promote rice plant growth

A study on the extent, aetiology and associated factors of dandruff in a group of medical students and the in vitro effects of antidandruff preparations

Introduction: Dandruff is a scalp disorder, characterized by itching and rapid skin flaking of the scalp of a person. Yeasts of the genus Malassezia are responsible for dandruff. The prevalence of dandruff in South Asia is 60.1%, whereas in the world it is 50%. The objectives of this study were to ascertain the prevalence, describe associated factors, isolate Malassezia sp. from dandruff samples of medical students and test the anti-fungal effects of commercially available shampoo and crude extracts of commonly used herbs against Malassezia. Methods: Ninety five students were chosen by systematic sampling. A self-administered questionnaire was used for data collection. Samples of skin flakes/swabs was collected from the scalp of participants. Specimens were examined using three direct microscopic methods. Fungal colonies isolated on Saborauds/Dixon Agar were identified using Gram stain and catalase test. Antifungal activity of six shampoo brands and crude extracts of eleven herbs were tested against isolated Malassezia sp. by agar well/disc diffusion method. Results: Prevalence of dandruff was 64.2% in the study group. The typical ‘spaghetti and meatball’ appearance was seen in the skin flakes taken from 44 of 61 (72.1%) students. Malassezia sp. was isolated only from 49 of the 61 students (80.3%). In-vitro, anti-malassezial effect (Inhibition zone (IZ)&gt;10mm) was observed in 4 of the 7 tested ‘anti dandruff’ shampoos. Crude extracts of fresh leaves of Psidium guavava (guava), flowers of Punica grantum (pomegranate) and fruits of Solanum lycopersicum (tomato) and Citrus hystrix (Kaffir lime) showed IZs &gt; 10mm. Conclusion: Prevalence of dandruff in the studied group of medical students was higher than in the Asian population. Four of the tested ‘anti dandruff’ shampoos and crude extracts of leaves of P. guavava (guava), fruits of S. lycopersicum (tomato), C. hystrix (kaffir lime), and flowers of P. grantum (pomegranate) showed an in-vitro inhibitory effect on the growth of Malassezia sp.</p

Routinely-collected general practice data are complex, but with systematic processing can be used for quality improvement and research.

UK general practice is computerised, and quality targets based on computer data provide a further incentive to improve data quality. A National Programme for Information Technology is standardising the technical infrastructure and removing some of the barriers to data aggregation. Routinely collected data is an underused resource, yet little has been written about the wide range of factors that need to be taken into account if we are to infer meaning from general practice data

Routinely-collected general practice data are complex, but with systematic processing can be used for quality improvement and research.

UK general practice is computerised, and quality targets based on computer data provide a further incentive to improve data quality. A National Programme for Information Technology is standardising the technical infrastructure and removing some of the barriers to data aggregation. Routinely collected data is an underused resource, yet little has been written about the wide range of factors that need to be taken into account if we are to infer meaning from general practice data

A study of cardiovascular risk in overweight and obese people in England.

To report current levels of obesity and associated cardiac risk using routinely collected primary care computer data

The ALFA (Activity Log Files Aggregation) Toolkit: A Method for Precise Observation of the Consultation

Background: There is a lack of tools to evaluate and compare Electronic patient record (EPR) systems to inform a rational choice or development agenda. Objective: To develop a tool kit to measure the impact of different EPR system features on the consultation. Methods: We first developed a specification to overcome the limitations of existing methods. We divided this into work packages: (1) developing a method to display multichannel video of the consultation; (2) code and measure activities, including computer use and verbal interactions; (3) automate the capture of nonverbal interactions; (4) aggregate multiple observations into a single navigable output; and (5) produce an output interpretable by software developers. We piloted this method by filming live consultations (n = 22) by 4 general practitioners (GPs) using different EPR systems. We compared the time taken and variations during coded data entry, prescribing, and blood pressure (BP) recording. We used nonparametric tests to make statistical comparisons. We contrasted methods of BP recording using Unified Modeling Language (UML) sequence diagrams. Results: We found that 4 channels of video were optimal. We identified an existing application for manual coding of video output. We developed in-house tools for capturing use of keyboard and mouse and to time stamp speech. The transcript is then typed within this time stamp. Although we managed to capture body language using pattern recognition software, we were unable to use this data quantitatively. We loaded these observational outputs into our aggregation tool, which allows simultaneous navigation and viewing of multiple files. This also creates a single exportable file in XML format, which we used to develop UML sequence diagrams. In our pilot, the GP using the EMIS LV (Egton Medical Information Systems Limited, Leeds, UK) system took the longest time to code data (mean 11.5 s, 95% CI 8.7-14.2). Nonparametric comparison of EMIS LV with the other systems showed a significant difference, with EMIS PCS (Egton Medical Information Systems Limited, Leeds, UK) (P = .007), iSoft Synergy (iSOFT, Banbury, UK) (P = .014), and INPS Vision (INPS, London, UK) (P = .006) facilitating faster coding. In contrast, prescribing was fastest with EMIS LV (mean 23.7 s, 95% CI 20.5-26.8), but nonparametric comparison showed no statistically significant difference. UML sequence diagrams showed that the simplest BP recording interface was not the easiest to use, as users spent longer navigating or looking up previous blood pressures separately. Complex interfaces with free-text boxes left clinicians unsure of what to add. Conclusions: The ALFA method allows the precise observation of the clinical consultation. It enables rigorous comparison of core elements of EPR systems. Pilot data suggests its capacity to demonstrate differences between systems. Its outputs could provide the evidence base for making more objective choices between systems. Keywords: Video recordings, process assessment, observation, attitude to computer, professional-patient relations, general practice, family practice, decision modeling, process assessment, medical informatics, computers, medical records systems, computerized, electronic patient record (EPR), electronic medical record (EMR), evaluation methodologies, usabilit