Identification of individual enzymes in crude extracts by acrylamide gel electrophoresis

Identification of enzymes by acrylamide gel electrophoresi

Some characteristics of adaptation in am1

Adaptation in am

Scalable iterative methods for sampling from massive Gaussian random vectors

Sampling from Gaussian Markov random fields (GMRFs), that is multivariate Gaussian ran- dom vectors that are parameterised by the inverse of their covariance matrix, is a fundamental problem in computational statistics. In this paper, we show how we can exploit arbitrarily accu- rate approximations to a GMRF to speed up Krylov subspace sampling methods. We also show that these methods can be used when computing the normalising constant of a large multivariate Gaussian distribution, which is needed for both any likelihood-based inference method. The method we derive is also applicable to other structured Gaussian random vectors and, in particu- lar, we show that when the precision matrix is a perturbation of a (block) circulant matrix, it is still possible to derive O(n log n) sampling schemes.Comment: 17 Pages, 4 Figure

Handling Qualities Assessment of a Pilot Cueing System for Autorotation Maneuvers

This paper details the design and limited flight testing of a preliminary system for visual pilot cueing during autorotation maneuvers. The cueing system is based on a fully-autonomous, multi-phase autorotation control law that has been shown to successfully achieve autonomous autorotation landing in unmanned helicopters. To transition this control law to manned systems, it is employed within a cockpit display to drive visual markers which indicate desired collective pitch and longitudinal cyclic positions throughout the entire maneuver, from autorotation entry to touchdown. A series of simulator flight experiments performed at University of Liverpool’s HELIFLIGHT-R simulator are documented, in which pilots attempt autorotation with and without the pilot cueing system in both good and degraded visual environments. Performance of the pilot cueing system is evaluated based on both subjective pilot feedback and objective measurements of landing survivability metrics, demonstrating suitable preliminary performance of the system

Feedback in the local LBG Analog Haro 11 as probed by far-UV and X-ray observations

We have re-analyzed FUSE data and obtained new Chandra observations of Haro 11, a local (D_L=88 Mpc) UV luminous galaxy. Haro 11 has a similar far-UV luminosity (10^10.3 L_\odot), UV surface brightness (10^9.4 L_\odot kpc^-2), SFR, and metallicity to that observed in Lyman Break Galaxies (LBGs). We show that Haro 11 has extended, soft thermal (kT~0.68 keV) X-ray emission with a luminosity and size which scales with the physical properties (e.g. SFR, stellar mass) of the host galaxy. An enhanced alpha/Fe, ratio of ~4 relative to solar abundance suggests significant supernovae enrichment. These results are consistent with the X-ray emission being produced in a shock between a supernovae driven outflow and the ambient material. The FUV spectra show strong absorption lines similar to those observed in LBG spectra. A blueshifted absorption component is identified as a wind outflowing at ~200-280 km/s. OVI\lambda\lambda1032,1038 emission, the dominant cooling mechanism for coronal gas at T~10^5.5 K is also observed. If associated with the outflow, the luminosity of the OVI emission suggests that <20% of the total mechanical energy from the supernovae and solar winds is being radiated away. This implies that radiative cooling through OVI is not significantly inhibiting the growth of the outflowing gas. In contradiction to the findings of Bergvall et al 2006, we find no convincing evidence of Lyman continuum leakage in Haro 11. We conclude that the wind has not created a `tunnel' allowing the escape of a significant fraction of Lyman continuum photons and place a limit on the escape fraction of f_{esc}<2%. Overall, both Haro 11 and a previously observed LBG analogue VV 114, provide an invaluable insight into the X-ray and FUV properties of high redshift LBGs.Comment: Accepted for publication in ApJ, 40 pages, 17 figure

Development of targeted messages to promote smoking cessation among construction trade workers

Blue-collar workers, particularly those in the construction trades, are more likely to smoke and have less success in quitting when compared with white-collar workers. Little is known about health communication strategies that might influence this priority population. This article describes our formative work to develop targeted messages to increase participation in an existing smoking cessation program among construction workers. Using an iterative and sequential mixed-methods approach, we explored the culture, health attitudes and smoking behaviors of unionized construction workers. We used focus group and survey data to inform message development, and applied audience segmentation methods to identify potential subgroups. Among 144 current smokers, 65% reported wanting to quit smoking in the next 6 months and only 15% had heard of a union-sponsored smoking cessation program, despite widespread advertising. We tested 12 message concepts and 26 images with the target audience to evaluate perceived relevance and effectiveness. Participants responded most favorably to messages and images that emphasized family and work, although responses varied by audience segments based on age and parental status. This study is an important step towards integrating the culture of a high-risk group into targeted messages to increase participation in smoking cessation activities

Laser Stabilization at 1536 nm Using Regenerative Spectral Hole Burning

Laser frequency stabilization giving a 500-Hz Allan deviation for a 2-ms integration time with drift reduced to 7 kHz/min over several minutes was achieved at 1536 nm in the optical communication band. A continuously regenerated spectral hole in the inhomogeneously broadened 4I15/2(1)!4I13/2(1) optical absorption of an Er31:Y2SiO5 crystal was used as the short-term frequency reference, while a variation on the locking technique allowed simultaneous use of the inhomogeneously broadened absorption line as a long-term reference. The reported frequency stability was achieved without vibration isolation. Spectral hole burning frequency stabilization provides ideal laser sources for high-resolution spectroscopy, real-time optical signal processing, and a range of applications requiring ultra-narrow-band light sources or coherent detection; the time scale for stability and the compatibility with spectral hole burning devices make this technique complementary to other frequency references for laser stabilization