Air and noise pollution effects on air quality in peacock paint industry, Ikot Ekan, Etinan, Akwa Ibom State, Nigeria

Eight sampling locations in the study area were assessed for some air pollutants, level of noise pollution and some meteorological parameters. Portable handheld air monitor was used to measure air pollutants, a sound level meter and anemometer for level of noise and meteorological parameters respectively. Results showed that the highest concentrations of Nitrogen dioxide, Sulphur dioxide, Hydrogen Sulphide and Suspended particulate matter were recorded in indoor II while those for Carbon monoxide and Chloride were recorded in outdoor I. The outdoor locations recorded higher mean noise level and temperature (76.1 dB (A), 30.05°C) than the indoor locations (70.95 dB (A), 27.03°C). On the other hand, the indoor locations recorded higher mean relative humidity (62.8%) than the outdoor locations (48.25%). Significant difference (P<0.05) was observed only in the mean concentration of Chloride in the two environments. The mean concentrations of NO2, SO2, H2S, CO and Cl2 were above the permissible limit of Federal Environmental Protection Agency thus posing environmental risks. Appropriate vehicle emission management should be considered; air and noise pollution control agencies should intervene to reduce the risks associate with these pollutants and improve the air quality of the study area

OPTICAL AND STRUCTURAL CHARACTERIZATION OF FECUS TERNARY THIN FILMS

Ternary thin films of Iron Copper Sulphide (FeCuS) have been grown and characterized. Optical, compositional, surface microstructure and structural characterization of the deposited films were carried out. Absorbance spectra data of the films were obtained using a Janway 6405 UV-VIS spectrophotometer, absorbance of the films were found to be high in UV and low in VIS – NIR region, while the transmittances were low in UV region and high in VIS – NIR regions. Elemental composition of the films were done using Skyray XRF Machine, EDX Pocket III, model P530. The machine was used to determine the percentage of each transition element present in the films. The surface microstructures of the films were characterized using Olympus Microscope at 100X magnification.  XRD analysis of the films was carried out using Enhance Mini Material Analyzer (EMMA) X – Ray Diffractometer Machine, the as - deposited FeCuS thin film was found to have tetragonal structure. The lattice constants obtained are , and . The crystallite size of the film was calculated at wavelength of and the calculated grain size was found to be . The optical absorption study reveals that FeCuS thin film has a bandgap of 2.40eV and a refractive index range of to at 400nm. The reflectance of the deposited films was found to be generally low

Effects of Planting Time and Poultry Manure on Late Season Plaintain Establishment and Yield in Owerri Rainforest Zone of Nigeria

In rainfed agriculture, plantain availability to consumer is seasonal. Late season plantain planting with appropriate organic manure application has been one of the advocated solutions. This experiment was carried out at the Federal University of Technology Teaching and Research  farm, Owerri in 2010 late season planting to determine the  appropriate time of planting and optimum manure rate that will enhance late season plantain establishment and bunch availability in the tropical rainforest. The experiment was a 3x4 factorial fitted into a randomized complete block design replicated three times. The treatments were three late season months (5th September, 5th October and 5thNovember) and four poultry manure rates (0,4,8 and 12 t.ha-1). The pre and post soil, establishment, growth and yield data were collected and analysed statistically.  The post soil chemical analysis showed an improvement on soil pH,(5.30-6.07, 5.59-5.67 and 5.73-5.72), organic matter,(2.098-2.098, 2.476-2.373 and 2.240-2.201%) calcium,(7.03-10.20, 3.60-6.40 and 5.40-5.70 l/kg) organic carbon,(1.696-1.217, 1.436-1.377 and1.436-1.277%)  phosphorous (21.39-20.86, 20.20-18.80 and14.60-13.90ppm)  and  magnesium (0.67-2.00, 0.83-1.17 and 0.83-1.00CMOL) in the treatment that received 8 and12 t ha-1 poultry manure in September, October and November planting respectively..  The residual effect of poultry manure resulted in increased yield (8.6, 7.2 and 7.0kg)  of the first ratoon crop in September, October, and November planting that received 12t ha-1poultry manure respectively.  Although poultry manure rates enhanced plantain establishment, growth and yield when planted in September, October and November, plantain planted  in September and manured with 12 t ha-1 poultry manure significantly(p=0.05) enhanced plantain establishment,(100%) ,yield (9.2kg in plant crop,8.6 first ratoon) and  income return (N 7,135,,501.8/ha) in Owerri Rainforest zone of Nigeria. Keywords: Planting time,  poultry manure, late season plantain, production, humid tropics

Endothelial Cell Processing and Alternatively Spliced Transcripts of Factor VIII: Potential Implications for Coagulation Cascades and Pulmonary Hypertension

Background: Coagulation factor VIII (FVIII) deficiency leads to haemophilia A. Conversely, elevated plasma levels are a strong predictor of recurrent venous thromboemboli and pulmonary hypertension phenotypes in which in situ thromboses are implicated. Extrahepatic sources of plasma FVIII are implicated, but have remained elusive. Methodology/Principal Findings: Immunohistochemistry of normal human lung tissue, and confocal microscopy, flow cytometry, and ELISA quantification of conditioned media from normal primary endothelial cells were used to examine endothelial expression of FVIII and coexpression with von Willebrand Factor (vWF), which protects secreted FVIII heavy chain from rapid proteloysis. FVIII transcripts predicted from database mining were identified by rt-PCR and sequencing. FVIII mAb-reactive material was demonstrated in CD31+ endothelial cells in normal human lung tissue, and in primary pulmonary artery, pulmonary microvascular, and dermal microvascular endothelial cells. In pulmonary endothelial cells, this protein occasionally colocalized with vWF, centered on Weibel Palade bodies. Pulmonary artery and pulmonary microvascular endothelial cells secreted low levels of FVIII and vWF to conditioned media, and demonstrated cell surface expression of FVIII and vWF Ab–reacting proteins compared to an isotype control. Four endothelial splice isoforms were identified. Two utilize transcription start sites in alternate 59 exons within the int22h-1 repeat responsible for intron 2

An MPEG-7 scheme for semantic content modelling and filtering of digital video

Abstract Part 5 of the MPEG-7 standard specifies Multimedia Description Schemes (MDS); that is, the format multimedia content models should conform to in order to ensure interoperability across multiple platforms and applications. However, the standard does not specify how the content or the associated model may be filtered. This paper proposes an MPEG-7 scheme which can be deployed for digital video content modelling and filtering. The proposed scheme, COSMOS-7, produces rich and multi-faceted semantic content models and supports a content-based filtering approach that only analyses content relating directly to the preferred content requirements of the user. We present details of the scheme, front-end systems used for content modelling and filtering and experiences with a number of users

Model construction of medical endoscope service evaluation system-based on the analysis of Delphi method

Background: Medical endoscope is widely used in clinical practice for the purpose of diagnosis and treatment, occupying around 5% of the medical device market. Evaluating the true service level of medical endoscope is essential and necessary to improve overall performance of medical diagnosis and treatment, and to maintain competitiveness of endoscope manufacturers, however, such a tool is not available in the market. This study develops an Evaluation Index System (EIS) to assess service level of medical endoscope, and to provide suggestions for improving the service level through the Delphi method. Methods: Firstly, the possible factors influencing the service level were identified from literature review. In parallel, the Delphi expert method questionnaire was designed and 25 experts were invited to conduct three rounds of questionnaire, to evaluate and rate the possible factors. Finally, we determined the weights associated with the factors, using the analytic hierarchy process (AHP) and percentage method, and developed the service level EIS. Results: The EIS consists of 3 first-level indicators, 24 s-level indicators and 68 third-level indicators. According to the weights computed using AHP, first-level indicators are ranked as post-sale (0.62), in-sale (0.25) and pre-sale (0.13). Through case verification, the medical endoscope brand Olympus had a total score of 4.17, Shanghai Aohua had a total score of 3.71, and Shanghai Chengyun had a total score of 3.28, which matches its market popularity and ranking in terms of market share. The results obtained from the EIS are consistent with the reality. Conclusions: The EIS established in this study is comprehensive, reliable and reasonable with strong practicality. The EIS can act as a tool for the endoscope users to evaluate potential products and make informed choices. It also provides a measurable basis for endoscope manufacturers and service providers to improve service quality

Validation of xMAP SARS-CoV-2 Multi-Antigen IgG assay in Nigeria

Objective: There is a need for reliable serological assays to determine accurate estimates of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence. Most single target antigen assays have shown some limitations in Africa. To assess the performance of a multi-antigen assay, we evaluated a commercially available SARS-CoV-2 Multi-Antigen IgG assay for human coronavirus disease 2019 (COVID-19) in Nigeria. / Methods: Validation of the xMAP SARS-CoV-2 Multi-Antigen IgG assay was carried out using well-characterized SARS-CoV-2 reverse transcription polymerase chain reactive positive (97) and pre-COVID-19 pandemic (86) plasma panels. Cross-reactivity was assessed using pre-COVID-19 pandemic plasma specimens (213) from the 2018 Nigeria HIV/AIDS Indicator and Impact Survey (NAIIS). / Results: The overall sensitivity of the xMAP SARS-CoV-2 Multi-Antigen IgG assay was 75.3% [95% CI: 65.8%– 82.8%] and specificity was 99.0% [95% CI: 96.8%– 99.7%]. The sensitivity estimate increased to 83.3% [95% CI: 70.4%– 91.3%] for specimens >14 days post-confirmation of diagnosis. However, using the NAIIS pre-pandemic specimens, the false positivity rate was 1.4% (3/213). / Conclusions: Our results showed overall lower sensitivity and a comparable specificity with the manufacturer’s validation. There appears to be less cross-reactivity with NAIIS pre-pandemic COVID-19 specimens using the xMAP SARS-CoV-2 Multi-Antigen IgG assay. In-country SARS-CoV-2 serology assay validation can help guide the best choice of assays in Africa

Antifungal Activity of Microbial Secondary Metabolites

Secondary metabolites are well known for their ability to impede other microorganisms. Reanalysis of a screen of natural products using the Caenorhabditis elegans-Candida albicans infection model identified twelve microbial secondary metabolites capable of conferring an increase in survival to infected nematodes. In this screen, the two compound treatments conferring the highest survival rates were members of the epipolythiodioxopiperazine (ETP) family of fungal secondary metabolites, acetylgliotoxin and a derivative of hyalodendrin. The abundance of fungal secondary metabolites indentified in this screen prompted further studies investigating the interaction between opportunistic pathogenic fungi and Aspergillus fumigatus, because of the ability of the fungus to produce a plethora of secondary metabolites, including the well studied ETP gliotoxin. We found that cell-free supernatant of A. fumigatus was able to inhibit the growth of Candida albicans through the production of a secreted product. Comparative studies between a wild-type and an A. fumigatus ΔgliP strain unable to synthesize gliotoxin demonstrate that this secondary metabolite is the major factor responsible for the inhibition. Although toxic to organisms, gliotoxin conferred an increase in survival to C. albicans-infected C. elegans in a dose dependent manner. As A. fumigatus produces gliotoxin in vivo, we propose that in addition to being a virulence factor, gliotoxin may also provide an advantage to A. fumigatus when infecting a host that harbors other opportunistic fungi

Investigating determinants of out-of-pocket spending and strategies for coping with payments for healthcare in southeast Nigeria

<p>Abstract</p> <p>Background</p> <p>Out-of-pocket spending (OOPS) is the major payment strategy for healthcare in Nigeria. Hence, the paper assessed the determinants socio-economic status (SES) of OOPS and strategies for coping with payments for healthcare in urban, semi-urban and rural areas of southeast Nigeria. This paper provides information that would be required to improve financial accessibility and equity in financing within the public health care system.</p> <p>Methods</p> <p>The study areas were three rural and three urban areas from Ebonyi and Enugu states in South-east Nigeria. Cross-sectional survey using interviewer-administered questionnaires to randomly selected householders was the study tool. A socio-economic status (SES) index that was developed using principal components analysis was used to examine levels of inequity in OOPS and regression analysis was used to examine the determinants of use of OOPS.</p> <p>Results</p> <p>All the SES groups equally sought healthcare when they needed to. However, the poorest households were most likely to use low level and informal providers such as traditional healers, whilst the least poor households were more likely to use the services of higher level and formal providers such as health centres and hospitals. The better-off SES more than worse-off SES groups used OOPS to pay for healthcare. The use of own money was the commonest payment-coping mechanism in the three communities. The sales of movable household assets or land were not commonly used as payment-coping mechanisms. Decreasing SES was associated with increased sale of household assets to cope with payment for healthcare in one of the communities. Fee exemptions and subsidies were almost non-existent as coping mechanisms in this study</p> <p>Conclusions</p> <p>There is the need to reduce OOPS and channel and improve equity in healthcare financing by designing and implementing payment strategies that will assure financial risk protection of the poor such pre-payment mechanisms with government paying for the poor.</p