636 research outputs found
An investigation of the blood supply to the long bones in the pig with particular reference to the arterial supply of the epiphysis and the epiphyseal plate
The Process of Developing a Multi-Cell KEMS Instrument
Multi-cell KEMS offers many advantages over single cell instruments in regard to in-situ temperature calibration and studies on high temperature alloys and oxides of interest to NASA. The instrument at NASA Glenn is a 90 deg magnetic sector instrument originally designed for single cell operation. The conversion of this instrument to a multi-cell instrument with restricted collimation is discussed. For restricted collimation, the 'field aperture' is in the copper plate separating the Knudsen Cell region and the ionizer and the 'source aperture' is adjacent to the ionizer box. A computer controlled x-y table allows positioning of one of the three cells into the sampling region. Heating is accomplished via a Ta sheet element and temperature is measured via an automatic pyrometer from the bottom of the cells. The computer control and data system have been custom developed for this instrument and are discussed. Future improvements are also discussed
Holographic Thermalization
Using the AdS/CFT correspondence, we probe the scale-dependence of
thermalization in strongly coupled field theories following a quench, via
calculations of two-point functions, Wilson loops and entanglement entropy in
d=2,3,4. In the saddlepoint approximation these probes are computed in AdS
space in terms of invariant geometric objects - geodesics, minimal surfaces and
minimal volumes. Our calculations for two-dimensional field theories are
analytical. In our strongly coupled setting, all probes in all dimensions share
certain universal features in their thermalization: (1) a slight delay in the
onset of thermalization, (2) an apparent non-analyticity at the endpoint of
thermalization, (3) top-down thermalization where the UV thermalizes first. For
homogeneous initial conditions the entanglement entropy thermalizes slowest,
and sets a timescale for equilibration that saturates a causality bound over
the range of scales studied. The growth rate of entanglement entropy density is
nearly volume-independent for small volumes, but slows for larger volumes.Comment: 39 pages, 24 figure
LOS oligosaccharide modification enhances dendritic cell responses to meningococcal native outer membrane vesicles expressing a non toxic lipid A.
Outer membrane vesicles (OMV) are released by many bacteria, and contain immunogenic antigens in addition to harmful inflammatory factors, like lipopolysaccharides. Chemically detoxified OMV have been used in vaccines against Neisseria meningitidis (Nm), however little is known about their interaction with antigen presenting cells. In this study, we investigated the interaction of Nm OMV with human dendritic cells (DC) to gain further understanding of their biological activity. We engineered a novel serogroup B Nm that is unencapsulated (siaD), expresses pentacylated lipid A (lpxL1), hence conferring reduced toxicity, and expresses an lgtB oligosaccharide structure designed to target OMV to DC via DC-SIGN. We show that the lgtB moiety is critical for internalization of NOMV by DC. Furthermore, the lgtB moiety significantly enhances DC maturation, IL-10 and IL-23 production in the presence of a pentacylated lipid A. Whilst different DC phenotypes were observed for each NOMV, this had little effect on Th1 and Th2 cell differentiation, however lgtB significantly increased Th17 cell expansion in the presence of pentacylated lipid A. We believe that lgtB/lpxL1 NOMV should be considered further as a vaccine vector, particularly considering the importance of lgtB in antigen uptake and further human studies on antigen specific responses should be considered
Ramond-Ramond Cohomology and O(D,D) T-duality
In the name of supersymmetric double field theory, superstring effective
actions can be reformulated into simple forms. They feature a pair of vielbeins
corresponding to the same spacetime metric, and hence enjoy double local
Lorentz symmetries. In a manifestly covariant manner --with regard to O(D,D)
T-duality, diffeomorphism, B-field gauge symmetry and the pair of local Lorentz
symmetries-- we incorporate R-R potentials into double field theory. We take
them as a single object which is in a bi-fundamental spinorial representation
of the double Lorentz groups. We identify cohomological structure relevant to
the field strength. A priori, the R-R sector as well as all the fermions are
O(D,D) singlet. Yet, gauge fixing the two vielbeins equal to each other
modifies the O(D,D) transformation rule to call for a compensating local
Lorentz rotation, such that the R-R potential may turn into an O(D,D) spinor
and T-duality can flip the chirality exchanging type IIA and IIB
supergravities.Comment: 1+37 pages, no figure; Structure reorganized, References added, To
appear in JHEP. cf. Gong Show of Strings 2012
(http://wwwth.mpp.mpg.de/members/strings/strings2012/strings_files/program/Talks/Thursday/Gongshow/Lee.pdf
MTSS1 is a critical epigenetically regulated tumor suppressor in CML
Chronic myeloid leukemia (CML) is driven by malignant stem cells that can persist despite therapy. We have identified Metastasis suppressor 1 (Mtss1/MIM) to be downregulated in hematopoietic stem and progenitor cells from leukemic transgenic SCLtTA/Bcr-Abl mice and in patients with CML at diagnosis, and Mtss1 was restored when patients achieved complete remission. Forced expression of Mtss1 decreased clonogenic capacity and motility of murine myeloid progenitor cells and reduced tumor growth. Viral transduction of Mtss1 into lineage depleted SCLtTA/Bcr-Abl bone marrow cells decreased leukemic cell burden in recipients, and leukemogenesis was reduced upon injection of Mtss1 overexpressing murine myeloid 32D cells. Tyrosine kinase inhibitor (TKI) therapy and reversion of Bcr-Abl expression increased Mtss1 expression but failed to restore it to control levels. CML patient samples revealed higher DNA methylation of specific Mtss1 promoter CpG sites that contain binding sites for Kaiso and Rest transcription factors. In summary, we identified a novel tumor suppressor in CML stem cells that is downregulated by both Bcr-Abl kinase-dependent and -independent mechanisms. Restored Mtss1 expression markedly inhibits primitive leukemic cell biology in vivo, providing a therapeutic rationale for the Bcr-Abl-Mtss1 axis to target TKI resistant CML stem cells in patients
Analysis of model rotor blade pressures during parallel interaction with twin vortices
This paper presents and provides analysis of unsteady surface pressures measured on a model rotor blade as the blade experienced near parallel blade vortex interaction with a twin vortex system. To provide a basis for analysis, the vortex system was characterized by hot-wire measurements made in the interaction plane but in the absence of the rotor. The unsteady pressure response resulting from a single vortex interaction is then presented to provide a frame of reference for the twin vortex results. A series of twin vortex interaction cases are then presented and analyzed. It is shown that the unsteady blade pressures and forces are very sensitive to the inclination angle and separation distance of the vortex pair. When the vortex cores lie almost parallel to the blade chord, the interaction is characterized by a two-stage response associated with the sequential passage of the two cores. Conversely, when the cores lie on a plane that is almost perpendicular to the blade chord, the response is similar to that of a single vortex interaction. In all cases, the normal force response is consistent with the distribution of vertical velocity in the flow field of the vortex system. The pitching moment response, on the other hand, depends on the localized suction associated with the vortex cores as they traverse the blade chord
On the Riemann Tensor in Double Field Theory
Double field theory provides T-duality covariant generalized tensors that are
natural extensions of the scalar and Ricci curvatures of Riemannian geometry.
We search for a similar extension of the Riemann curvature tensor by developing
a geometry based on the generalized metric and the dilaton. We find a duality
covariant Riemann tensor whose contractions give the Ricci and scalar
curvatures, but that is not fully determined in terms of the physical fields.
This suggests that \alpha' corrections to the effective action require \alpha'
corrections to T-duality transformations and/or generalized diffeomorphisms.
Further evidence to this effect is found by an additional computation that
shows that there is no T-duality invariant four-derivative object built from
the generalized metric and the dilaton that reduces to the square of the
Riemann tensor.Comment: 36 pages, v2: minor changes, ref. added, v3: appendix on frame
formalism added, version to appear in JHE
Mucosal Therapy of Multi-Drug Resistant Tuberculosis With IgA and Interferon-γ
New evidence has been emerging that antibodies can be protective in various experimental models of tuberculosis. Here, we report on protection against multidrug-resistant Mycobacterium tuberculosis (MDR-TB) infection using a combination of the human monoclonal IgA 2E9 antibody against the alpha-crystallin (Acr, HspX) antigen and mouse interferon-gamma in mice transgenic for the human IgA receptor, CD89. The effect of the combined mucosal IgA and IFN-γ; treatment was strongest (50-fold reduction) when therapy was applied at the time of infection, but a statistically significant reduction of lung bacterial load was observed even when the therapy was initiated once the infection had already been established. The protection involving enhanced phagocytosis and then neutrophil mediated killing of infected cells was IgA isotype mediated, because treatment with an IgG version of 2E9 antibody was not effective in human IgG receptor CD64 transgenic mice. The Acr antigen specificity of IgA antibodies for protection in humans has been indicated by their elevated serum levels in latent tuberculosis unlike the lack of IgA antibodies against the virulence-associated MPT64 antigen. Our results represent the first evidence for potential translation of mucosal immunotherapy for the management of MDR-TB
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