3-D Hand Pose Estimation from Kinect's Point Cloud Using Appearance Matching

We present a novel appearance-based approach for pose estimation of a human hand using the point clouds provided by the low-cost Microsoft Kinect sensor. Both the free-hand case, in which the hand is isolated from the surrounding environment, and the hand-object case, in which the different types of interactions are classified, have been considered. The hand-object case is clearly the most challenging task having to deal with multiple tracks. The approach proposed here belongs to the class of partial pose estimation where the estimated pose in a frame is used for the initialization of the next one. The pose estimation is obtained by applying a modified version of the Iterative Closest Point (ICP) algorithm to synthetic models to obtain the rigid transformation that aligns each model with respect to the input data. The proposed framework uses a "pure" point cloud as provided by the Kinect sensor without any other information such as RGB values or normal vector components. For this reason, the proposed method can also be applied to data obtained from other types of depth sensor, or RGB-D camera

Differential sensitivity of brainstem vs cortical astrocytes to changes in pH reveals functional regional specialization of astroglia

Astrocytes might function as brain interoceptors capable of detecting different (chemo)sensory modalities and transmitting sensory information to the relevant neural networks controlling vital functions. For example, astrocytes which reside near the ventral surface of the brainstem (central respiratory chemosensitive area) respond to physiological decreases in pH with vigorous elevations in intracellular Ca(2+) and release of ATP. ATP transmits astroglial excitation to the brainstem respiratory network and contributes to adaptive changes in lung ventilation. Here we show that in terms of pH-sensitivity ventral brainstem astrocytes are clearly distinct from astrocytes residing in the cerebral cortex. We monitored vesicular fusion in cultured rat brainstem astrocytes using total internal reflection fluorescence microscopy and found that approximately 35% of them respond to acidification with an increased rate of exocytosis of ATP-containing vesicular compartments. These fusion events require intracellular Ca(2+) signaling and are independent of autocrine ATP actions. In contrast, the rate of vesicular fusion in cultured cortical astrocytes is not affected by changes in pH. Compared to cortical astrocytes, ventral brainstem astrocytes display higher levels of expression of genes encoding proteins associated with ATP vesicular transport and fusion, including vesicle-associated membrane protein-3 and vesicular nucleotide transporter. These results suggest that astrocytes residing in different parts of the rat brain are functionally specialized. In contrast to cortical astrocytes, astrocytes of the brainstem chemosensitive area(s) possess signaling properties which are functionally relevant – they are able to sense changes in pH and respond to acidification with enhanced vesicular release of ATP

Screening and attraction of dust particles in plasmas.

The potential around a dust particle in a plasma is found using the collisional hydrodynamic equations of dusty plasmas, taking into account ion-dust and ion-neutral collisions and considering the plasma source proportional to the dust density. The linear screening is strongly influenced by the collisions and can substantially differ from Debye screening. Attraction of negatively charged dust particles can occur due to overscreening by the ion fluxes in the presence of friction forces

Do Patients with Bronchiectasis Have an Increased Risk of Developing Lung Cancer? A Systematic Review

Background: Initial evidence supports the hypothesis that patients with non-cystic fibrosis bronchiectasis (NCFB) have a higher risk of lung cancer. We systematically reviewed the available literature to define the characteristics of lung malignancies in patients with bronchiectasis and the characteristics of patients who develop bronchiectasis-associated lung cancer. Method: This study was performed based on the PRISMA guidelines. The review protocol was registered in PROSPERO. Results: The frequency rates of lung cancer in patients with NCFB ranged from 0.93% to 8.0%. The incidence rate was 3.96. Cancer more frequently occurred in the elderly and males. Three studies found an overall higher risk of developing lung cancer in the NCFB population compared to the non-bronchiectasis one, and adenocarcinoma was the most frequently reported histological type. The effect of the co-existence of NCFB and COPD was unclear. Conclusions: NCFB is associated with a higher risk of developing lung cancer than individuals without NCFB. This risk is higher for males, the elderly, and smokers, whereas concomitant COPD’s effect is unclear

Endoscopic Technologies for Peripheral Pulmonary Lesions: From Diagnosis to Therapy

Peripheral pulmonary lesions (PPLs) are frequent incidental findings in subjects when performing chest radiographs or chest computed tomography (CT) scans. When a PPL is identified, it is necessary to proceed with a risk stratification based on the patient profile and the characteristics found on chest CT. In order to proceed with a diagnostic procedure, the first-line examination is often a bronchoscopy with tissue sampling. Many guidance technologies have recently been developed to facilitate PPLs sampling. Through bronchoscopy, it is currently possible to ascertain the PPL’s benign or malignant nature, delaying the therapy’s second phase with radical, supportive, or palliative intent. In this review, we describe all the new tools available: from the innovation of bronchoscopic instrumentation (e.g., ultrathin bronchoscopy and robotic bronchoscopy) to the advances in navigation technology (e.g., radial-probe endobronchial ultrasound, virtual navigation, electromagnetic navigation, shape-sensing navigation, cone-beam computed tomography). In addition, we summarize all the PPLs ablation techniques currently under experimentation. Interventional pulmonology may be a discipline aiming at adopting increasingly innovative and disruptive technologies

Human biomonitoring of t-2 toxin, t-2 toxin-3-glucoside and their metabolites in urine through high-resolution mass spectrometry

The metabolic profile of T-2 toxin (T-2) and its modified form T-2-3-glucoside (T-2-3-Glc) remain unexplored in human samples. Therefore, the present study aimed to investigate the presence of T-2, T-2-3-Glc and their respective major metabolites in human urine samples (n = 300) collected in South Italy through an ultra-high performance liquid chromatography (UHPLC) coupled to Q-Orbitrap-HRMS methodology. T-2 was quantified in 21% of samples at a mean concentration of 1.34 ng/mg Crea (range: 0.22–6.54 ng/mg Crea). Almost all the major T-2 metabolites previously characterized in vitro were tentatively found, remarking the occurrence of 3′-OH-T-2 (99.7%), T-2 triol (56%) and HT-2 (30%). Regarding T-2-3-Glc, a low prevalence of the parent mycotoxin (1%) and its metabolites were observed, with HT-2-3-Glc (17%) being the most prevalent compound, although hydroxylated products were also detected. Attending to the large number of testing positive for T-2 or its metabolites, this study found a frequent exposure in Italian population

Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway

Ergothioneine (Egt) is a dietary amino acid which acts as an antioxidant to protect against ageing-related diseases. We investigated the anti-cancer properties of Egt in colorectal cancer cells (CRC). Egt treatment exerted cytotoxicity in a dose-dependent manner, induced reactive oxygen species accumulation, loss of mitochondrial membrane potential and upregulation of the histone deacetylase SIRT3. Immunoblotting analysis indicated that the cell death occurred via necroptosis through activation of the RIP1/RIP3/MLKL pathway. An immunoprecipitation assay unveiled that the interaction between the terminal effector in necroptotic signalling MLKL and SIRT3 increased during the Egt treatment. SIRT3 gene silencing blocked the upregulation of MLKL and abolished the ability of Egt to induce necroptosis. The SIRT3–MLKL interaction may mediate the necroptotic effects of Egt in CRC, suggesting the potential of this dietary amino thione in the prevention of CRC

SCREENING TOMATO GLUTATHIONE S-TRANSFERASE DIVERSITY FOR ASSOCIATION TO DROUGHT STRESS

Environmental stresses are one of the main challenges in agriculture, as they significantly limit crop productivity. As a result of global warming and climate change, extreme environmental events and abiotic stresses are expected to increase in intensity and frequency. Selecting tolerant plants for more resilient agro-ecosystems is a promising strategy for mitigating the effects of adverse environments. The cultivated tomato (Solanum lycopersicum L.) has a narrow genetic base, and its reduced genetic diversity hampers the progress of breeding. However, the rediscovery and use of local and wild genetic resources allow useful/beneficial alleles to be identified and used in breeding programs. To improve adaptation to harmful conditions, plants have evolved many molecular and genetics mechanisms, including hormone regulation and the activation of specific gene networks. Among others, glutathione S-transferase (GST) genes have been identified in various plant species and found to be involved in different physiological, developmental and stress modulation pathways. The aim of this research is to add insights into the role of tomato GST genes in the plant response to stress by associating allele diversity with drought tolerance. Tomato GST sequences were characterized in silico showing their diffuse clustering pattern across chromosomes with TAU class GSTs duplicated in tandem and mostly aggregated on chromosomes 7 and 9. To characterize the diversity of selected GSTs, a core collection of 75 tomato accessions was selected based on microsatellite screening from a larger collection of worldwide accessions. The core collection was screened for drought tolerance when 50% of plants showed fruit set on the lower flower truss. At this stage, two different water treatments were applied consisting in the complete restitution of the water lost due to evapo-transpiration (Full Water Restitution FWR) and the restitution of 50% of the water lost (Half Water Restitution HWR). After seven days the plants showed visible sign of drought and the leaves were analyzed for gas exchange and colorimetric variations. A gas exchange analyzer (Licor 6400) was used to measure the assimilation rate of foliar CO2 (µmol CO2 m-2 s-1) and transpiration (mmol H2O m-2 s-1). The colorimetric analysis was carried out with a portable Chroma Meters (Minolta). The eco-physiological data showed a continuous variability of the response to drought throughout the collection and allowed to identify eight tomato accessions combining a lower reduction in CO2 assimilation, transpiration and water use efficiency and higher stability in leaf temperatures and colorimetric variations when challenged with HWR. Enriched libraries for GST loci were prepared based on the specific hybridization of short oligonucleotide primers (20-25 bases). Libraries will be sequenced using next/third generation sequencing technology (Illumina or PacBio). Mapped variants and haplotypes in the GST loci will be integrated with drought tolerant traits and used to identify associations with tolerant phenotypes to additional environmental stresses

Clinical pharmacogenetics of methotrexate

It is well known that interindividual variability can affect the response to many drugs in relation to age, gender, diet, and organ function. Pharmacogenomic studies have also documented that genetic polymorphisms can exert clinically significant effects in terms of drug resistance, efficacy and toxicity by modifying the expression of critical gene products (drug-metabolizing enzymes, transporters, and target molecules) as well as pharmacokinetic and pharmacodynamic parameters. A growing body of in vitro and clinical evidence suggests that common polymorphisms in the folate gene pathway are associated with an altered response to methotrexate (MTX) in patients with malignancy and autoimmune disease. Such polymorphisms may also induce significant MTX toxicity requiring expensive monitoring and treatment. Although the available data are not conclusive, they suggest that in the future MTX pharmacogenetics could play a key role in clinical practice by improving and tailoring treatment. This review describes the genetic polymorphisms that significantly influence MTX resistance, efficacy, and toxicity