Optimizing the tracking of gestation in the equine industry: An analysis of the links between the pH and chemical composition of mare's milk and the onset of parturition

Purpose of Study: The purpose of this study was to determine if pH is a viable and cost effective factor to use in the prediction of parturition in mares.Background: The tracking of pH levels is a promising indicator of the onset of parturition in mares. As this study was performed on only one mare, more data will need to be collected from a larger sample size in order to know if this would be a cost effective predictor of parturition in mares.Methods: The milk from a brood mare was collected, tested, and compared using an inexpensive over-the-counter pH test strip, a pH meter, and a Redox SYS oxidative-reductive testing machine. The samples were taken using sterile techniques, while trying to control for as many variables as possible, in order to obtain better comparative data. The samples also were tested for ORP and calcium hardness concentrations.Results: There was a strong correlation (r=0.9187) at (P<0.01) and a significant P value of 0.000024 found between the pH testing methods utilized in this study. Furthermore, there was a consistency of calcium hardness concentrations prior to parturition, followed by a significant spike in those values at the time of parturition and continuing after the event.Conclusion: The results of this study indicate that mare milk pH does drop prior to parturition and could be a reliable indicator of parturition occurring within 24-48 hours of such noted drops even when tested with the over-the-counter pH strips. Testing with such over-the-counter pH strips would make tracking such drops cost effective in production settings

Orienteering with One Endomorphism

In supersingular isogeny-based cryptography, the path-finding problem reduces to the endomorphism ring problem. Can path-finding be reduced to knowing just one endomorphism? It is known that a small endomorphism enables polynomial-time path-finding and endomorphism ring computation (Love-Boneh [36]). An endomorphism gives an explicit orientation of a supersingular elliptic curve. In this paper, we use the volcano structure of the oriented supersingular isogeny graph to take ascending/descending/horizontal steps on the graph and deduce path-finding algorithms to an initial curve. Each altitude of the volcano corresponds to a unique quadratic order, called the primitive order. We introduce a new hard problem of computing the primitive order given an arbitrary endomorphism on the curve, and we also provide a sub-exponential quantum algorithm for solving it. In concurrent work (Wesolowski [54]), it was shown that the endomorphism ring problem in the presence of one endomorphism with known primitive order reduces to a vectorization problem, implying path-finding algorithms. Our path-finding algorithms are more general in the sense that we don't assume the knowledge of the primitive order associated with the endomorphism.Comment: 40 pages, 1 figure; 3rd revision implements small corrections and expositional improvement

Orientations and cycles in supersingular isogeny graphs

The paper concerns several theoretical aspects of oriented supersingular $\ell$-isogeny volcanoes and their relationship to closed walks in the supersingular $\ell$-isogeny graph. Our main result is a bijection between the rims of the union of all oriented supersingular $\ell$-isogeny volcanoes over $\overline{\mathbb{F}}_p$ (up to conjugation of the orientations), and isogeny cycles (non-backtracking closed walks which are not powers of smaller walks) of the supersingular $\ell$-isogeny graph over $\overline{\mathbb{F}}_p$. The exact proof and statement of this bijection are made more intricate by special behaviours arising from extra automorphisms and the ramification of $p$ in certain quadratic orders. We use the bijection to count isogeny cycles of given length in the supersingular $\ell$-isogeny graph exactly as a sum of class numbers of these orders, and also give an explicit upper bound by estimating the class numbers

Tribological investigation of graphene as lubricant additive in vegetable oil

In this study, the tribological behaviours of graphene nanoparticles as lubricant additive in vegetable oil were investigated as a function of nanoparticle concentration. Graphene nanoparticles were added at 25 ppm, 50 ppm and 100 ppm as additive into vegetable oil as lubricant through hydrodynamic and acoustic cavitation as homogenising mechanism. The tribological properties of each homogenised solution were tested using a four-ball machine according to ASTM D4172. Wear scar diameter and friction coefficient were the determining parameters in this study. The results showed that 50 ppm is the optimum concentration that recorded the lowest wear scar diameter and friction coefficient as compared to control sample

Direct Visualization of Laser-Driven Electron Multiple Scattering and Tunneling Distance in Strong-Field Ionization

Using a simple model of strong-field ionization of atoms that generalizes the well-known 3-step model from 1D to 3D, we show that the experimental photoelectron angular distributions resulting from laser ionization of xenon and argon display prominent structures that correspond to electrons that pass by their parent ion more than once before strongly scattering. The shape of these structures can be associated with the specific number of times the electron is driven past its parent ion in the laser field before scattering. Furthermore, a careful analysis of the cutoff energy of the structures allows us to experimentally measure the distance between the electron and ion at the moment of tunnel ionization. This work provides new physical insight into how atoms ionize in strong laser fields and has implications for further efforts to extract atomic and molecular dynamics from strong-field physics

Participation of the Cell Polarity Protein PALS1 to T-Cell Receptor-Mediated NF-κB Activation

BACKGROUND: Beside their established function in shaping cell architecture, some cell polarity proteins were proposed to participate to lymphocyte migration, homing, scanning, as well as activation following antigen receptor stimulation. Although PALS1 is a central component of the cell polarity network, its expression and function in lymphocytes remains unknown. Here we investigated whether PALS1 is present in T cells and whether it contributes to T Cell-Receptor (TCR)-mediated activation. METHODOLOGY/PRINCIPAL FINDINGS: By combining RT-PCR and immunoblot assays, we found that PALS1 is constitutively expressed in human T lymphocytes as well as in Jurkat T cells. siRNA-based knockdown of PALS1 hampered TCR-induced activation and optimal proliferation of lymphocyte. We further provide evidence that PALS1 depletion selectively hindered TCR-driven activation of the transcription factor NF-κB. CONCLUSIONS: The cell polarity protein PALS1 is expressed in T lymphocytes and participates to the optimal activation of NF-κB following TCR stimulation

Correlation of Group C Meningococcal Conjugate Vaccine Response with B- and T-Lymphocyte Activity

Despite the success of conjugate vaccination against meningococcal group C (MenC) disease, post-vaccination, some individuals still exhibit rapid waning of initially protective bactericidal antibody levels. The mechanism of this relative loss of humoral protection remains undetermined. In this report we have investigated the relationship between T- and B-cell activation and co-stimulation and the loss of protective antibody titers. We have found that healthy volunteers who lose protective MenC antibody levels one year after receipt of glycoconjugate vaccine exhibit no detectable cellular defect in polyclonal B- or T-cell activation, proliferation or the B-memory pool. This suggests that the processes underlying the more rapid loss of antibody levels are independent of defects in either initial T- or B-cell activation

Identification and Evolution of Drug Efflux Pump in Clinical Enterobacter aerogenes Strains Isolated in 1995 and 2003

BACKGROUND: The high mortality impact of infectious diseases will increase due to accelerated evolution of antibiotic resistance in important human pathogens. Development of antibiotic resistance is a evolutionary process inducing the erosion of the effectiveness of our arsenal of antibiotics. Resistance is not necessarily limited to a single class of antibacterial agents but may affect many unrelated compounds; this is termed 'multidrug resistance' (MDR). The major mechanism of MDR is the active expulsion of drugs by bacterial pumps; the treatment of gram negative bacterial infections is compromised due to resistance mechanisms including the expression of efflux pumps that actively expel various usual antibiotics (beta-lactams, quinolones, ...). METHODOLOGY/PRINCIPAL FINDINGS: Enterobacter aerogenes has emerged among Enterobacteriaceae associated hospital infections during the last twenty years due to its faculty of adaptation to antibiotic stresses. Clinical isolates of E. aerogenes belonging to two strain collections isolated in 1995 and 2003 respectively, were screened to assess the involvement of efflux pumps in antibiotic resistance. Drug susceptibility assays were performed on all bacterial isolates and an efflux pump inhibitor (PAbetaN) previously characterized allowed to decipher the role of efflux in the resistance. Accumulation of labelled chloramphenicol was monitored in the presence of an energy poison to determine the involvement of active efflux on the antibiotic intracellular concentrations. The presence of the PAbetaN-susceptible efflux system was also identified in resistant E. aerogenes strains. CONCLUSIONS/SIGNIFICANCE: For the first time a noticeable increase in clinical isolates containing an efflux mechanism susceptible to pump inhibitor is report within an 8 year period. After the emergence of extended spectrum beta-lactamases in E. aerogenes and the recent characterisation of porin mutations in clinical isolates, this study describing an increase in inhibitor-susceptible efflux throws light on a new step in the evolution of mechanism in E. aerogenes