Nutritional Status of Households of Rural Field Practice Area of a Tertiary Care Hospital in India

Introduction: In the world as a whole there appears to be a shift from under-nourishment towards over-nourishment making more and more children, adolescents, adults and even elderly to be overweight and obese. Objectives: Study aimed to find out the age and sex wise commonness of over-weight & obesity amongst the families of an overtly different socio-economic environment and its trend in the members of one type of families. Materials & Methods: The undergraduate medical students are supposed to maintain record of individual health (including height & weight) of their own family as well as that of the allotted family. The data collected (record maintained ) by students was utilized to calculate Body Mass Index (BMI). Results: Out of total 291 subjects (males 168; females 123) in students own family 28.9% (28.0%; 30.1%) were overweight and 5.9% (6.0%; 5.7%) were obese. The similar figures for 262 subjects (males 143 & females 119) in the allotted families were 20.2% (18.5%; 20.2%) and 6.5% (4.2%; 8.4%) respectively. The respective percentages of under nourished individuals were 18.6 (17.9; 19.5) and 35.5 (37.8; 32.8). Thus over-nutrition was more common amongst the members of students own families (34.8% vs. 26.7%) and under-nutrition was more common amongst the members of allotted families (35.5% vs. 18.6%) For the years 2000-2003, BMI amongst individuals of students own families the under-nutrition in the age group of 15-24 years amongst males increased from 15.9% to 32.9% and over-nutrition from 13.6% to 20.5%. There was no case of overweight and obesity up to the age of 34 years in the previous analysis which was 2.6% in the present analysis Previous results demonstrated overweight to be more common in males (32.4% Vs. 24.4% in females) and obesity being more common females ( 6.3% Vs. 2.6% in females). Conclusion: Males are increasingly becoming prey of malnutrition (adolescents for under-nutrition and adults & elderly for over-nutrition. More studies covering larger samples are required to be conducted on a more frequent basis

Comparison of the Effects of Coconut Oil and Soyabean Oil on TSH Level and Weight Gain in Rabbits

The present study was conducted on 12 albino rabbits of either sex and weighing between 1-1.5kg to see the influence of coconut oil and soyabean oil on serum TSH levels and weight gain for a period of 12 weeks.  The rabbits were divided into 2 groups of six each.  Rabbits in group 1 were fed on coconut oil and in group 2 were fed on soyabean oil in addition to their standard diet.  At the end of 12 weeks we found that rabbits fed on soyabean oil had significant increase in TSH levels (p= 0.003) and gained more weight (p=0.000) when compared to rabbits fed on coconut oil

Driver seat comfort for level 3-4 autonomous vehicles

Autonomous vehicles can classified on a scale from 0 to 5, where level 0 corresponds to vehicles that have no automation to level 5 where the vehicle is fully autonomous and it is not possible for the human occupant to take control. At level 2, the driver needs to retain attention as they are in control of at least some systems. Level 3-4 vehicles are capable of full control but the human occupant might be required to, or desire to, intervene in some circumstances. This means that there could be extended periods of time where the driver is relaxed, but other periods of time when they need to drive. The seat must therefore be designed to be comfortable in at least two different types of use case. This driving simulator study compares the comfort experienced in a seat from a production hybrid vehicle whilst being used in a manual driving mode and in autonomous mode for a range of postures. It highlights how discomfort is worse for cases where the posture is non-optimal for the task. It also investigates the design of head and neckrests to mitigate neck discomfort, and shows that a well-designed neckrest is beneficial for drivers in autonomous mode

Driver seat comfort for level 3-4 autonomous vehicles

Background: Autonomous vehicles can be classified on a scale of automation from 0 to 5, where level 0 corresponds to vehicles that have no automation to level 5 where the vehicle is fully autonomous and it is not possible for the human occupant to take control. At level 2, the driver needs to retain attention as they are in control of at least some systems. Level 3-4 vehicles are capable of full control but the human occupant might be required to, or desire to, intervene in some circumstances. This means that there could be extended periods of time where the driver is relaxed, but other periods of time when they need to drive. Objective: The seat must therefore be designed to be comfortable in at least two different types of use case. Methods: This driving simulator study compares the comfort experienced in a seat from a production hybrid vehicle whilst being used in a manual driving mode and in autonomous mode for a range of postures. Results: It highlights how discomfort is worse for cases where the posture is non-optimal for the task. It also investigates the design of head and neckrests to mitigate neck discomfort, and shows that a well-designed neckrest is beneficial for drivers in autonomous mode

Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells

We examined the effect of intact human mast cells (HMC-1 5C6) and their selected mediators on interleukin-6 (IL-6) production and bone morphogenetic protein-2 (BMP-2) expression in human coronary artery endothelial cells (HCAEC) in the presence and absence of lipopolysaccharide (LPS). Scanning electron microscopy showed that HMC-1 5C6 cells adhere to HCAEC in cocultures. Addition of HMC-1 5C6 cells markedly enhanced the IL-6 production by quiescent and LPS-activated HCAEC even at the maximal concentration of LPS. Furthermore, mast cell-derived histamine and proteases accounted for the direct and synergistic effect of mast cells on IL-6 production that was completely blocked by the combination of histamine receptor-1 antagonist and protease inhibitors. Another novel finding is that histamine was able to induce BMP-2 expression in HCAEC. Collectively, our results suggest that endotoxin and mast cell products synergistically amplify vascular inflammation and that histamine participates in the early events of vascular calcification

Reducing auditory nerve excitability by acute antagonism of Ca2+-permeable AMPA receptors

Hearing depends on glutamatergic synaptic transmission mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs). AMPARs are tetramers, where inclusion of the GluA2 subunit reduces overall channel conductance and C

Insights into the Regulatory Characteristics of the Mycobacterial Dephosphocoenzyme A Kinase: Implications for the Universal CoA Biosynthesis Pathway

Being vastly different from the human counterpart, we suggest that the last enzyme of the Mycobacterium tuberculosis Coenzyme A biosynthetic pathway, dephosphocoenzyme A kinase (CoaE) could be a good anti-tubercular target. Here we describe detailed investigations into the regulatory features of the enzyme, affected via two mechanisms. Enzymatic activity is regulated by CTP which strongly binds the enzyme at a site overlapping that of the leading substrate, dephosphocoenzyme A (DCoA), thereby obscuring the binding site and limiting catalysis. The organism has evolved a second layer of regulation by employing a dynamic equilibrium between the trimeric and monomeric forms of CoaE as a means of regulating the effective concentration of active enzyme. We show that the monomer is the active form of the enzyme and the interplay between the regulator, CTP and the substrate, DCoA, affects enzymatic activity. Detailed kinetic data have been corroborated by size exclusion chromatography, dynamic light scattering, glutaraldehyde crosslinking, limited proteolysis and fluorescence investigations on the enzyme all of which corroborate the effects of the ligands on the enzyme oligomeric status and activity. Cysteine mutagenesis and the effects of reducing agents on mycobacterial CoaE oligomerization further validate that the latter is not cysteine-mediated or reduction-sensitive. These studies thus shed light on the novel regulatory features employed to regulate metabolite flow through the last step of a critical biosynthetic pathway by keeping the latter catalytically dormant till the need arises, the transition to the active form affected by a delicate crosstalk between an essential cellular metabolite (CTP) and the precursor to the pathway end-product (DCoA)

Kaposi's Sarcoma-Associated Herpesvirus Forms a Multimolecular Complex of Integrins ( V 5, V 3, and 3 1) and CD98-xCT during Infection of Human Dermal Microvascular Endothelial Cells, and CD98-xCT Is Essential for the Postentry Stage of Infection

Kaposi's sarcoma-associated herpesvirus (KSHV) interacts with cell surface heparan sulfate (HS) and α3β1 integrin during the early stages of infection of human dermal microvascular endothelial cells (HMVEC-d) and human foreskin fibroblasts (HFF), and these interactions are followed by virus entry overlapping with the induction of preexisting host cell signal pathways. KSHV also utilizes the amino acid transporter protein xCT for infection of adherent cells, and the xCT molecule is part of the cell surface heterodimeric membrane glycoprotein CD98 (4F2 antigen) complex known to interact with α3β1 and αVβ3 integrins. KSHV gB mediates adhesion of HMVEC-d, CV-1, and HT-1080 cells and HFF via its RGD sequence. Anti-αV and -β1 integrin antibodies inhibited the cell adhesion mediated by KSHV-gB. Variable levels of neutralization of HMVEC-d and HFF infection were observed with antibodies against αVβ3 and αVβ5 integrins. Similarly, variable levels of inhibition of virus entry into adherent HMVEC-d, 293 and Vero cells, and HFF was observed by preincubating virus with soluble α3β1, αVβ3, and αVβ5 integrins, and cumulative inhibition was observed with a combination of integrins. We were unable to infect HT1080 cells. Virus binding and DNA internalization studies suggest that αVβ3 and αVβ5 integrins also play roles in KSHV entry. We observed time-dependent temporal KSHV interactions with HMVEC-d integrins and CD98/xCT with three different patterns of association and dissociation. Integrin αVβ5 interaction with CD98/xCT predominantly occurred by 1 min postinfection (p.i.) and dissociated at 10 min p.i., whereas α3β1-CD98/xCT interaction was maximal at 10 min p.i. and dissociated at 30 min p.i., and αVβ3-CD98/xCT interaction was maximal at 10 min p.i. and remained at the observed 30 min p.i. Fluorescence microscopy also showed a similar time-dependent interaction of αVβ5-CD98. Confocal-microscopy studies confirmed the association of CD98/xCT with α3β1 and KSHV. Preincubation of KSHV with soluble heparin and α3β1 significantly inhibited this association, suggesting that the first contact with HS and integrin is an essential element in subsequent CD98-xCT interactions. Anti-CD98 and xCT antibodies did not block virus binding and entry and nuclear delivery of viral DNA; however, viral-gene expression was significantly inhibited, suggesting that CD98-xCT play roles in the post-entry stage of infection, possibly in mediating signal cascades essential for viral-gene expression. Together, these studies suggest that KSHV interacts with functionally related integrins (αVβ3, α3β1, and αVβ5) and CD98/xCT molecules in a temporal fashion to form a multimolecular complex during the early stages of endothelial cell infection, probably mediating multiple roles in entry, signal transduction, and viral-gene expression