35 research outputs found
Bragg spectroscopy with an accelerating Bose-Einstein condensate
We present the results of Bragg spectroscopy performed on an accelerating
Bose-Einstein condensate. The Bose condensate undergoes circular micro-motion
in a magnetic TOP trap and the effect of this motion on the Bragg spectrum is
analyzed. A simple frequency modulation model is used to interpret the observed
complex structure, and broadening effects are considered using numerical
solutions to the Gross-Pitaevskii equation.Comment: 5 pages, 3 figures, to appear in PRA. Minor changes to text and fig
Studying Children's Intrapersonal Emotion Regulation Strategies from the Process Model of Emotion Regulation
peerreview_statement: The publishing and review policy for this title is described in its Aims & Scope. aims_and_scope_url: http://www.tandfonline.com/action/journalInformation?show=aimsScope&journalCode=vgnt2
Cross-oncopanel study reveals high sensitivity and accuracy with overall analytical performance depending on genomic regions
BackgroundTargeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food and Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation of eight Pan-Cancer panels to assess best practices for oncopanel sequencing.ResultsAll panels demonstrate high sensitivity across targeted high-confidence coding regions and variant types for the variants previously verified to have variant allele frequency (VAF) in the 5-20% range. Sensitivity is reduced by utilizing VAF thresholds due to inherent variability in VAF measurements. Enforcing a VAF threshold for reporting has a positive impact on reducing false positive calls. Importantly, the false positive rate is found to be significantly higher outside the high-confidence coding regions, resulting in lower reproducibility. Thus, region restriction and VAF thresholds lead to low relative technical variability in estimating promising biomarkers and tumor mutational burden.ConclusionThis comprehensive study provides actionable guidelines for oncopanel sequencing and clear evidence that supports a simplified approach to assess the analytical performance of oncopanels. It will facilitate the rapid implementation, validation, and quality control of oncopanels in clinical use.Peer reviewe