Contagem de células somáticas, perfil de sensibilidade antimicrobiana e microorganismos isolados de mastites em búfalos: uma breve revisão.

Resumo: Apesar de haver alguns mitos sobre a espécie bubalina, esses animais apresentam problemas sanitários semelhantes aos dos bovinos, dentre eles a ocorrência de casos de mastite. Embora algumas particularidades relacionadas a características anatômicas do úbere e imunologia da glândula mamária proporcionem maior resistência contra a infecção, búfalas com elevada contagem de células somáticas (CCS) apresentam redução da produção de leite e alterações dos teores de seus constituintes. Esta revisão teve como objetivo levantar informações sobre o padrão de CCS normalmente encontrado para a espécie, os microorganismos mais frequentemente isolados e a resistência microbiana frente à antibióticoterapia. Abstract: Despite there are some myths about the buffalo, these animals have health problems similar to those of cattle, among them the occurrence of mastitis. Although some peculiarities related to anatomical features of the udder and immunology of the mammary gland provide greater resistance to infection, buffaloes with high somatic cell count (SCC) present lower productivity and changes in milk constituent?s levels. This review aimed to gather information about pattern of SCC usually found for specie, most frequently isolated microorganisms and microbial resistance against antibiotic therapy

A geo-chemo-mechanical study of a highly polluted marine system (Taranto, Italy) for the enhancement of the conceptual site model

The paper presents the results of the analysis of the geo-chemo-mechanical data gathered through an innovative multidisciplinary investigation campaign in the Mar Piccolo basin, a heavily polluted marine bay aside the town of Taranto (Southern Italy). The basin is part of an area declared at high environmental risk by the Italian government. The cutting-edge approach to the environmental characterization of the site was promoted by the Special Commissioner for urgent measures of reclamation, environmental improvements and redevelopment of Taranto and involved experts from several research fields, who cooperated to gather a new insight into the origin, distribution, mobility and fate of the contaminants within the basin. The investigation campaign was designed to implement advanced research methodologies and testing strategies. Differently from traditional investigation campaigns, aimed solely at the assessment of the contamination state within sediments lying in the top layers, the new campaign provided an interpretation of the geo-chemo-mechanical properties and state of the sediments forming the deposit at the seafloor. The integrated, multidisciplinary and holistic approach, that considered geotechnical engineering, electrical and electronical engineering, geological, sedimentological, mineralogical, hydraulic engineering, hydrological, chemical, geochemical, biological fields, supported a comprehensive understanding of the influence of the contamination on the hydro-mechanical properties of the sediments, which need to be accounted for in the selection and design of the risk mitigation measures. The findings of the research represent the input ingredients of the conceptual model of the site, premise to model the evolutionary contamination scenarios within the basin, of guidance for the environmental risk management. The study testifies the importance of the cooperative approach among researchers of different fields to fulfil the interpretation of complex polluted eco-systems

Contagem de células somáticas, perfil de sensibilidade antimicrobiana e microorganismos isolados de mastites em búfalos: uma breve revisão.

Resumo: Apesar de haver alguns mitos sobre a espécie bubalina, esses animais apresentam problemas sanitários semelhantes aos dos bovinos, dentre eles a ocorrência de casos de mastite. Embora algumas particularidades relacionadas a características anatômicas do úbere e imunologia da glândula mamária proporcionem maior resistência contra a infecção, búfalas com elevada contagem de células somáticas (CCS) apresentam redução da produção de leite e alterações dos teores de seus constituintes. Esta revisão teve como objetivo levantar informações sobre o padrão de CCS normalmente encontrado para a espécie, os microorganismos mais frequentemente isolados e a resistência microbiana frente à antibióticoterapia. Abstract: Despite there are some myths about the buffalo, these animals have health problems similar to those of cattle, among them the occurrence of mastitis. Although some peculiarities related to anatomical features of the udder and immunology of the mammary gland provide greater resistance to infection, buffaloes with high somatic cell count (SCC) present lower productivity and changes in milk constituent?s levels. This review aimed to gather information about pattern of SCC usually found for specie, most frequently isolated microorganisms and microbial resistance against antibiotic therapy.201

Neuroleptic induced parkinsonism: MRI findings in relation to clinical course after withdrawal of neuroleptic drugs.

Parkinsonism is a common complication of neuroleptic drug use; however, the pathophysiology of the persistence of parkinsonian symptoms after withdrawal of neuroleptic drugs is poorly understood. Twenty patients with neuroleptic induced parkinsonism were studied by high field MRI. Persistence of symptoms was associated with different findings depending on the age of the patients--namely, putaminal hypointensity in young patients and striatal hyperintensities in old patients. High field MRI may be useful in identifying patients at higher risk for neuroleptic induced parkinsonism

Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2

Pitx2 is a bicoid-related homeobox transcription factor implicated in regulating left-right patterning and organogenesis. However, only a limited number of Pitx2 downstream target genes have been identified and characterized. Here we demonstrate that Pitx2 is a transcriptional repressor of DEP domain containing 1B (DEPDC1B). The first intron of the human and mouse DEP domain containing 1B genes contains multiple consensus DNA-binding sites for Pitx2. Chromatin immunoprecipitation assays revealed that Pitx2, along with histone deacetylase 1, was recruited to the first intron of Depdc1b. In contrast, RNAi-mediated depletion of Pitx2 not only enhanced the acetylation of histone H4 in the first intron of Depdc1b, but also increased the protein level of Depdc1b. Luciferase reporter assays also showed that Pitx2 could repress the transcriptional activity mediated by the first intron of human DEPDC1B. The GAP domain of DEPDC1B interacted with nucleotide-bound forms of RAC1 in vitro. In addition, exogenous expression of DEPDC1B suppressed RAC1 activation and interfered with actin polymerization induced by the guanine nucleotide exchange factor TRIO. Moreover, DEPDC1B interacted with various signaling molecules such as U2af2, Erh, and Salm. We propose that Pitx2-mediated repression of Depdc1b expression contributes to the regulation of multiple molecular pathways, such as Rho GTPase signaling