Stand Density in South Florida Tropical Forests: Implications for the Function and Management of Everglades Tree Islands

Within the continental US, the broadleaved forests of south Florida are exceptional in the abundance and diversity of tree species of tropical origin. Dry tropical forests are regionally most extensive in the upper Florida Keys, but are also represented on the mainland as fragments on limestone rocklands, and as “tree islands” embedded in the Everglades marsh. The exposed Everglades tree islands have a history of human use reaching back thousands of years, and are subject to frequent disturbance from tropical storms and hurricanes. They are sensitive to the hydrology of the surrounding marsh, which can lead to gradual changes in species composition or stand structure, or to the sudden loss of the woody component entirely, especially when low water tables are precursors to damaging fires. Tree islands serve as local hotspots of biodiversity, and as concentrators of phosphorus in a landscape defined by P-limitation. The mechanisms by which P reaches the tree islands and is sequestered there are complex and not completely understood, but may depend in part on transpiration and resupply of water from the adjacent wetlands. Since transpiration is a direct function of the transpiring leaf surface, which itself is expected to vary with stocking, we examined the relationship between leaf area index and stand density in 16 Everglades tree islands. To determine maximum stocking levels for such forests, we also calculated stand density for tropical forests throughout south Florida, using a protocol modified slightly from Woodard et al. 2003. Our results suggest that (1) stand density in many Everglades tree islands is well below the expressed potential of similar tropical assemblages, (2) low site occupancy may prevent such under-stocked forests from performing several ecosystem functions, and (3) stand density can serve as an effective metric of forest condition for management or restoration purposes

Transgenic plants as a sustainable, terrestrial source of fish oils

An alternative, sustainable source of omega-3 long chain polyunsaturated fatty acids is widely recognized as desirable, helping to reduce pressure on current sources (wild capture fisheries) and providing a de novo source of these health beneficial fatty acids. This review will consider the efforts and progress to develop transgenic plants as terrestrial sources of omega-3 fish oils, focusing on recent developments and the possible explanations for advances in the field. We also consider the utility of such a source for use in aquaculture, since this industry is the major consumer of oceanic supplies of omega-3 fish oils. Given the importance of the aquaculture industry in meeting global requirements for healthy foodstuffs, an alternative source of omega-3 fish oils represents a potentially significant breakthrough for this production system. Transgenic Camelina seeds engineered to accumulate the omega-3 fatty acids EPA and DHA, represent a sustainable alternative to fish oils

Field trial evaluation of the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Camelina sativa: making fish oil substitutes in plants

6 páginas, 2 figurasThe global consumption of fish oils currently exceeds one million tonnes, with the natural de novo source of these important fatty acids forming the base of marine foodwebs. Here we describe the first field-based evaluation of a terrestrial source of these essential nutrients, synthesised in the seeds of transgenic Camelina sativa plants via the heterologous reconstitution of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway. Our data demonstrate the robust nature of this novel trait, and the feasibility of making fish oils in genetically modified crops. Moreover, to our knowledge, this is the most complex example of plant genetic engineering to undergo environmental release and field evaluation.Rothamsted Research receives grand-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC), UK. This study was supported via the Strategic Programme Grant BBS/E/C/00005207. We thank the staff of the Rothamsted Experimental Farm for their help in the management of this experimental trial. We acknowledge BASF Plant Sciences for generous provision of the vector system used in this study.Peer reviewe

Mitochondrial DNA Haplogroup JT is Related to Impaired Glycaemic Control and Renal Function in Type 2 Diabetic Patients

The association between mitochondrial DNA (mtDNA) haplogroup and risk of type 2 diabetes (T2D) is undetermined and controversial. This study aims to evaluate the impact of the main mtDNA haplogroups on glycaemic control and renal function in a Spanish population of 303 T2D patients and 153 healthy controls. Anthropometrical and metabolic parameters were assessed and mtDNA haplogroup was determined in each individual. Distribution of the different haplogroups was similar in diabetic and healthy populations and, as expected, T2D patients showed poorer glycaemic control and renal function than controls. T2D patients belonging to the JT haplogroup (polymorphism m.4216T>C) displayed statistically significant higher levels of fasting glucose and HbA(1c) than those of the other haplogroups, suggesting a poorer glycaemic control. Furthermore, diabetic patients with the JT haplogroup showed a worse kidney function than those with other haplogroups, evident by higher levels of serum creatinine, lower estimated glomerular filtration rate (eGFR), and slightly higher (although not statistically significant) urinary albumin-to-creatinine ratio. Our results suggest that JT haplogroup (in particular, change at position 4216 of the mtDNA) is associated with poorer glycaemic control in T2D, which can trigger the development of diabetic nephropathy

Progesterone analogue protects stressed photoreceptors via bFGF-mediated calcium influx.

Retinitis pigmentosa (RP) is a degenerative retinal disease leading to photoreceptor cell loss. In 2011, our group identified the synthetic progesterone ‘Norgestrel’ as a potential treatment for RP. Subsequent research showed Norgestrel to work through progesterone receptor membrane component 1 (PGRMC1) activation and upregulation of neuroprotective basic fibroblast growth factor (bFGF). Using trophic factor deprivation of 661W photoreceptor-like cells, we aimed to further elucidate the mechanism leading to Norgestrel-induced neuroprotection. In the present manuscript, we show by flow cytometry and live-cell immunofluorescence that Norgestrel induces an increase in cytosolic calcium in both healthy and stressed 661Ws over 24h. Specific PGRMC1 inhibition by AG205 (1 μM) showed this rise to be PGRMC1-dependent, primarily utilising calcium from extracellular sources, for blockade of L-type calcium channels by verapamil (50 μM) prevented a Norgestrel-induced calcium influx in stressed cells. Calcium influx was also shown to be bFGF-dependent, for siRNA knock down of bFGF prevented Norgestrel-PGRMC1 induced changes in cytosolic calcium. Notably, we demonstrate PGRMC1-activation is necessary for Norgestrel-induced bFGF upregulation. We propose that Norgestrel protects through the following pathway: binding to and activating PGRMC1 expressed on the surface of photoreceptor cells, PGRMC1 activation drives bFGF upregulation and subsequent calcium influx. Importantly, raised intracellular calcium is critical to Norgestrel's protective efficacy, for extracellular calcium chelation by EGTA abrogates the protective effects of Norgestrel on stressed 661W cells in vitro

The Burst Cluster: Dark Matter in a Cluster Merger Associated with the Short Gamma Ray Burst, GRB 050509B

We have identified a merging galaxy cluster with evidence of two distinct sub-clusters. The X-ray and optical data suggest that the subclusters are moving away from each other after closest approach. This cluster merger was discovered from observations of the well localized short-duration gamma-ray burst (GRB), GRB 050509B. The Swift/Burst Alert Telescope (BAT) source position is coincident with a cluster of galaxies ZwCl 1234.0+02916. The subsequent Swift/X-Ray Telescope (XRT) localization of the X-ray afterglow found the GRB coincident with 2MASX J12361286+2858580, a giant red elliptical galaxy in the cluster. Deep multi-epoch optical images were obtained to constrain the evolution of the GRB afterglow, including a 27480s exposure in the F814W band with Hubble Space Telescope Advanced Camera for Surveys (ACS), among the deepest imaging ever obtained towards a known galaxy cluster in a single passband. We perform a weak gravitational lensing analysis, including mapping the total mass distribution of the merger system. Combined with Chandra X-ray Observatory and Swift/XRT observations, we investigate the dynamical state of the merger to probe the nature of the dark matter component. Our weak gravitational lensing measurements reveal a separation of the X-ray centroid of the western subcluster from the center of the mass and galaxy light distributions, similar to that of the famous "Bullet cluster". We conclude that the "Burst cluster" is another candidate merger system for determining the nature of dark matter and for studying the environment of short GRBs. We discuss connections between the cluster dynamical state and/or matter composition and compact object mergers, the leading model for the origin of short GRBs. Finally, we present results from a weak lensing survey based on archival Very Large Telescope (VLT) images in the areas of 5 other short GRBs.Comment: 17 pages, 7 figures, accepted by Ap

miR-21 Promotes Fibrogenesis in Peritoneal Dialysis.

Peritoneal dialysis (PD) is a life-saving form of renal replacement therapy for those with end-stage kidney disease. Mesothelial cells (MCs) line the peritoneal cavity and help define peritoneal response to treatment-associated injury, a major reason for treatment failure. miRNAs are important regulators, but their roles in peritoneal fibrosis are largely unknown. In this study, miR-21 was one of the most abundant miRNAs in primary MCs, and was up-regulated by the profibrotic cytokine transforming growth factor-β1 and in PD effluent-derived MCs exhibiting mesenchymal phenotypic change. Increased miR-21 was found in peritoneal membrane biopsy specimens from PD patients compared to healthy controls (PD biocompatible, 5.86×, P = 0.0001; PD conventional, 7.09×, P < 0.0001, n = 11 per group). In PD effluent from a cohort of 230 patients, miR-21 was higher in those receiving the therapy long-term compared to new starters (n = 230, miR-21 3.26×, P = 0.001) and associated with icodextrin use (R = 0.52; 95% CI, 0.20-0.84), peritonitis count (R = 0.16; 95% CI, 0.03-0.29), and dialysate cytokines. miR-21 down-regulated programmed cell death 4 and programmed cell death 4 protein was decreased in peritoneal membrane biopsy specimens from PD patients compared to healthy controls. New miR-21 targets were identified that may be important during PD fibrogenesis. These data identify miR-21 as an important effector of fibrosis in the peritoneal membrane, and a promising biomarker in the dialysis effluent for membrane change in patients receiving PD